Identifying peroxidases and their oxidants in the early pathology of cystic fibrosis.
We aimed to determine whether myeloperoxidase (MPO) is the main peroxidase present in the airways of children with cystic fibrosis (CF) and to assess which oxidants it produces and whether they are associated with clinical features of CF. Children with CF (n=54) and without CF (n=16) underwent bronc...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2010
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/76863 |
| _version_ | 1848763779716743168 |
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| author | Thomson, E. Brennan, S. Senthilmohan, R. Gangell, C. Chapman, A. Sly, P. Kettle, A. Balding, E. Berry, L. Carlin, J. Carzino, R. de Klerk, N. Douglas, T. Foo, C. Garratt, L. Hall, Graham Harrison, J. Kicic, Anthony Laing, I. Logie, K. Massie, J. Mott, L. Murray, C. Parsons, F. Pillarisetti, N. Poreddy, S. Ranganathan, S. Robertson, C. Robins-Browne, R. Robinson, P. Skoric, B. Stick, S. Sutanto, E. Williamson, E. |
| author_facet | Thomson, E. Brennan, S. Senthilmohan, R. Gangell, C. Chapman, A. Sly, P. Kettle, A. Balding, E. Berry, L. Carlin, J. Carzino, R. de Klerk, N. Douglas, T. Foo, C. Garratt, L. Hall, Graham Harrison, J. Kicic, Anthony Laing, I. Logie, K. Massie, J. Mott, L. Murray, C. Parsons, F. Pillarisetti, N. Poreddy, S. Ranganathan, S. Robertson, C. Robins-Browne, R. Robinson, P. Skoric, B. Stick, S. Sutanto, E. Williamson, E. |
| author_sort | Thomson, E. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | We aimed to determine whether myeloperoxidase (MPO) is the main peroxidase present in the airways of children with cystic fibrosis (CF) and to assess which oxidants it produces and whether they are associated with clinical features of CF. Children with CF (n=54) and without CF (n=16) underwent bronchoscopy and bronchoalveolar lavage (BAL) for assessment of pulmonary infection and inflammation. BAL fluid was analyzed for MPO, halogenated tyrosines as markers of hypohalous acids, thiocyanate, and protein carbonyls. MPO was the only peroxidase detected in BAL samples from children with CF and its concentration was markedly higher than in controls. Levels of 3-chlorotyrosine and 3-bromotyrosine in proteins were higher in the CF group. They correlated with neutrophils and MPO. The concentration of thiocyanate in BAL samples was below 1μM. Protein carbonyl levels correlated with MPO and halogenated tyrosines in patients with CF. Levels of MPO and halogenated tyrosines were higher in children with infections, especially Pseudomonas aeruginosa, and in the presence of respiratory symptoms. They also correlated with the Kanga clinical score. Our findings suggest that MPO produces hypobromous acid as well as hypochlorous acid in the airways of children with CF and that these oxidants are involved in the early pathogenesis of CF. |
| first_indexed | 2025-11-14T11:08:53Z |
| format | Journal Article |
| id | curtin-20.500.11937-76863 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | eng |
| last_indexed | 2025-11-14T11:08:53Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-768632019-11-14T02:07:21Z Identifying peroxidases and their oxidants in the early pathology of cystic fibrosis. Thomson, E. Brennan, S. Senthilmohan, R. Gangell, C. Chapman, A. Sly, P. Kettle, A. Balding, E. Berry, L. Carlin, J. Carzino, R. de Klerk, N. Douglas, T. Foo, C. Garratt, L. Hall, Graham Harrison, J. Kicic, Anthony Laing, I. Logie, K. Massie, J. Mott, L. Murray, C. Parsons, F. Pillarisetti, N. Poreddy, S. Ranganathan, S. Robertson, C. Robins-Browne, R. Robinson, P. Skoric, B. Stick, S. Sutanto, E. Williamson, E. Bronchoalveolar Lavage Fluid Child Child, Preschool Cystic Fibrosis Disease Progression Female Humans Infant Inflammation Male Neutrophils Oxidation-Reduction Peroxidase Pseudomonas aeruginosa Respiratory Tract Infections Tyrosine We aimed to determine whether myeloperoxidase (MPO) is the main peroxidase present in the airways of children with cystic fibrosis (CF) and to assess which oxidants it produces and whether they are associated with clinical features of CF. Children with CF (n=54) and without CF (n=16) underwent bronchoscopy and bronchoalveolar lavage (BAL) for assessment of pulmonary infection and inflammation. BAL fluid was analyzed for MPO, halogenated tyrosines as markers of hypohalous acids, thiocyanate, and protein carbonyls. MPO was the only peroxidase detected in BAL samples from children with CF and its concentration was markedly higher than in controls. Levels of 3-chlorotyrosine and 3-bromotyrosine in proteins were higher in the CF group. They correlated with neutrophils and MPO. The concentration of thiocyanate in BAL samples was below 1μM. Protein carbonyl levels correlated with MPO and halogenated tyrosines in patients with CF. Levels of MPO and halogenated tyrosines were higher in children with infections, especially Pseudomonas aeruginosa, and in the presence of respiratory symptoms. They also correlated with the Kanga clinical score. Our findings suggest that MPO produces hypobromous acid as well as hypochlorous acid in the airways of children with CF and that these oxidants are involved in the early pathogenesis of CF. 2010 Journal Article http://hdl.handle.net/20.500.11937/76863 10.1016/j.freeradbiomed.2010.07.010 eng restricted |
| spellingShingle | Bronchoalveolar Lavage Fluid Child Child, Preschool Cystic Fibrosis Disease Progression Female Humans Infant Inflammation Male Neutrophils Oxidation-Reduction Peroxidase Pseudomonas aeruginosa Respiratory Tract Infections Tyrosine Thomson, E. Brennan, S. Senthilmohan, R. Gangell, C. Chapman, A. Sly, P. Kettle, A. Balding, E. Berry, L. Carlin, J. Carzino, R. de Klerk, N. Douglas, T. Foo, C. Garratt, L. Hall, Graham Harrison, J. Kicic, Anthony Laing, I. Logie, K. Massie, J. Mott, L. Murray, C. Parsons, F. Pillarisetti, N. Poreddy, S. Ranganathan, S. Robertson, C. Robins-Browne, R. Robinson, P. Skoric, B. Stick, S. Sutanto, E. Williamson, E. Identifying peroxidases and their oxidants in the early pathology of cystic fibrosis. |
| title | Identifying peroxidases and their oxidants in the early pathology of cystic fibrosis. |
| title_full | Identifying peroxidases and their oxidants in the early pathology of cystic fibrosis. |
| title_fullStr | Identifying peroxidases and their oxidants in the early pathology of cystic fibrosis. |
| title_full_unstemmed | Identifying peroxidases and their oxidants in the early pathology of cystic fibrosis. |
| title_short | Identifying peroxidases and their oxidants in the early pathology of cystic fibrosis. |
| title_sort | identifying peroxidases and their oxidants in the early pathology of cystic fibrosis. |
| topic | Bronchoalveolar Lavage Fluid Child Child, Preschool Cystic Fibrosis Disease Progression Female Humans Infant Inflammation Male Neutrophils Oxidation-Reduction Peroxidase Pseudomonas aeruginosa Respiratory Tract Infections Tyrosine |
| url | http://hdl.handle.net/20.500.11937/76863 |