Are stem cell characteristics altered by disease state?
Autologous stem cell transplantation combined with gene therapy can potentially be used to treat genetically inherited diseases. However, characterization of multipotential cells from a disease state remains extremely limited. We have characterized adult bone marrow stromal cells (MSCs) derived from...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | English |
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MARY ANN LIEBERT, INC
2005
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/76838 |
| _version_ | 1848763772808724480 |
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| author | Kicic, Anthony Hall, C.M. Shen, W.Y. Rakoczy, P.E. |
| author_facet | Kicic, Anthony Hall, C.M. Shen, W.Y. Rakoczy, P.E. |
| author_sort | Kicic, Anthony |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Autologous stem cell transplantation combined with gene therapy can potentially be used to treat genetically inherited diseases. However, characterization of multipotential cells from a disease state remains extremely limited. We have characterized adult bone marrow stromal cells (MSCs) derived from three retinal degenerative mouse models and compared them to marrow stromal cells derived from their normal strain counterparts. Despite similar profiles soon after harvest, at 30 days post-isolation, marrow stromal cells derived from a disease origin were shown to contain a large pool (∼89-99%) of undifferentiated marrow stromal cells (CD90+/STRO-1+) as compared to their normal counterparts (∼19-43%). Fetal bovine serum appeared essential for marrow stromal cell proliferation and was not found to induce differentiation, although it could be substituted with other additives including epidermal growth factor (EGF), platelet-derived growth factor (PDGF), and leukemia inhibitory factor (LIF). We also showed that resulting CD90+/STRO+cells derived from both states could be directed into desired lineages expressing at the same rate and that they could be transduced with the same efficiency using different viral vehicles. This investigation has shown the existence of a large pool of undifferentiated stem cells derived from the disease state that have the potential to form the desired cell types when appropriately cued. © Mary Ann Liebert, Inc. |
| first_indexed | 2025-11-14T11:08:47Z |
| format | Journal Article |
| id | curtin-20.500.11937-76838 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:08:47Z |
| publishDate | 2005 |
| publisher | MARY ANN LIEBERT, INC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-768382019-11-14T01:59:51Z Are stem cell characteristics altered by disease state? Kicic, Anthony Hall, C.M. Shen, W.Y. Rakoczy, P.E. Science & Technology Life Sciences & Biomedicine Cell & Tissue Engineering Hematology Medicine, Research & Experimental Transplantation Cell Biology Research & Experimental Medicine MARROW STROMAL CELLS HUMAN BONE-MARROW IN-VITRO NONHEMATOPOIETIC TISSUES HEMATOPOIETIC-CELLS IMMUNE-RESPONSES TRANSGENIC MICE VIRAL-ANTIGENS RAT RETINA ADULT-RAT Autologous stem cell transplantation combined with gene therapy can potentially be used to treat genetically inherited diseases. However, characterization of multipotential cells from a disease state remains extremely limited. We have characterized adult bone marrow stromal cells (MSCs) derived from three retinal degenerative mouse models and compared them to marrow stromal cells derived from their normal strain counterparts. Despite similar profiles soon after harvest, at 30 days post-isolation, marrow stromal cells derived from a disease origin were shown to contain a large pool (∼89-99%) of undifferentiated marrow stromal cells (CD90+/STRO-1+) as compared to their normal counterparts (∼19-43%). Fetal bovine serum appeared essential for marrow stromal cell proliferation and was not found to induce differentiation, although it could be substituted with other additives including epidermal growth factor (EGF), platelet-derived growth factor (PDGF), and leukemia inhibitory factor (LIF). We also showed that resulting CD90+/STRO+cells derived from both states could be directed into desired lineages expressing at the same rate and that they could be transduced with the same efficiency using different viral vehicles. This investigation has shown the existence of a large pool of undifferentiated stem cells derived from the disease state that have the potential to form the desired cell types when appropriately cued. © Mary Ann Liebert, Inc. 2005 Journal Article http://hdl.handle.net/20.500.11937/76838 10.1089/scd.2005.14.15 English MARY ANN LIEBERT, INC restricted |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Cell & Tissue Engineering Hematology Medicine, Research & Experimental Transplantation Cell Biology Research & Experimental Medicine MARROW STROMAL CELLS HUMAN BONE-MARROW IN-VITRO NONHEMATOPOIETIC TISSUES HEMATOPOIETIC-CELLS IMMUNE-RESPONSES TRANSGENIC MICE VIRAL-ANTIGENS RAT RETINA ADULT-RAT Kicic, Anthony Hall, C.M. Shen, W.Y. Rakoczy, P.E. Are stem cell characteristics altered by disease state? |
| title | Are stem cell characteristics altered by disease state? |
| title_full | Are stem cell characteristics altered by disease state? |
| title_fullStr | Are stem cell characteristics altered by disease state? |
| title_full_unstemmed | Are stem cell characteristics altered by disease state? |
| title_short | Are stem cell characteristics altered by disease state? |
| title_sort | are stem cell characteristics altered by disease state? |
| topic | Science & Technology Life Sciences & Biomedicine Cell & Tissue Engineering Hematology Medicine, Research & Experimental Transplantation Cell Biology Research & Experimental Medicine MARROW STROMAL CELLS HUMAN BONE-MARROW IN-VITRO NONHEMATOPOIETIC TISSUES HEMATOPOIETIC-CELLS IMMUNE-RESPONSES TRANSGENIC MICE VIRAL-ANTIGENS RAT RETINA ADULT-RAT |
| url | http://hdl.handle.net/20.500.11937/76838 |