Comparison of techniques for obtaining lower airway epithelial cells from children
Airway epithelial cells (AECs) are important in asthma as they are the first cells to encounter pathogens/allergens. In children, AECs can be obtained using a "blind" nonbronchoscopic technique through an endotracheal tube. However, due to the increasing use of laryngeal masks the number o...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | English |
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EUROPEAN RESPIRATORY SOC JOURNALS LTD
2008
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| Online Access: | http://hdl.handle.net/20.500.11937/76827 |
| _version_ | 1848763770529120256 |
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| author | McNamara, P.S. Kicic, Anthony Sutanto, E.N. Stevens, P.T. Stick, S.M. |
| author_facet | McNamara, P.S. Kicic, Anthony Sutanto, E.N. Stevens, P.T. Stick, S.M. |
| author_sort | McNamara, P.S. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Airway epithelial cells (AECs) are important in asthma as they are the first cells to encounter pathogens/allergens. In children, AECs can be obtained using a "blind" nonbronchoscopic technique through an endotracheal tube. However, due to the increasing use of laryngeal masks the number of children in whom this technique is applicable has become limited. Recently, the present authors began to use a portable "bronchoscope-directed" technique to sample AECs. The current study compares both techniques in both asthmatic and nonasthmatic children. A total of 81 children undergoing elective surgery, were grouped according to atopic status and respiratory symptoms. Cellular yield of blind and bronchoscope-directed brushings were compared and immunocytochemistry performed. AECs were cultured and cytokine analysis of culture supernatant undertaken. Both techniques were equally well-tolerated, with the only adverse effect being a cough in 10% of the subjects. The mean±SD cell yield was higher in bronchoscope-directed than blind brushings (5.1±2.4 versus 3.1±1.4×10 6 cells). Immunocytochemistry confirmed an epithelial cell lineage. Culture supernatant cytokine concentrations were similar regardless of sampling technique with patterns preserved between asthmatic and healthy nonatopic phenotypes. Compared with blind brushing portable bronchoscope-directed brushing is well-tolerated, yields significantly more cells and is a potentially quick and useful technique for obtaining airway epithelial cells for research into childhood respiratory disease, specifically asthma. Copyright©ERS Journals Ltd 2008. |
| first_indexed | 2025-11-14T11:08:44Z |
| format | Journal Article |
| id | curtin-20.500.11937-76827 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:08:44Z |
| publishDate | 2008 |
| publisher | EUROPEAN RESPIRATORY SOC JOURNALS LTD |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-768272019-11-12T08:20:29Z Comparison of techniques for obtaining lower airway epithelial cells from children McNamara, P.S. Kicic, Anthony Sutanto, E.N. Stevens, P.T. Stick, S.M. Science & Technology Life Sciences & Biomedicine Respiratory System airway epithelial cells bronchoscopy brush biopsy child PROTECTED-SPECIMEN BRUSH CYSTIC-FIBROSIS ASTHMA PNEUMONIA SAFETY BIOPSY SIZE Airway epithelial cells (AECs) are important in asthma as they are the first cells to encounter pathogens/allergens. In children, AECs can be obtained using a "blind" nonbronchoscopic technique through an endotracheal tube. However, due to the increasing use of laryngeal masks the number of children in whom this technique is applicable has become limited. Recently, the present authors began to use a portable "bronchoscope-directed" technique to sample AECs. The current study compares both techniques in both asthmatic and nonasthmatic children. A total of 81 children undergoing elective surgery, were grouped according to atopic status and respiratory symptoms. Cellular yield of blind and bronchoscope-directed brushings were compared and immunocytochemistry performed. AECs were cultured and cytokine analysis of culture supernatant undertaken. Both techniques were equally well-tolerated, with the only adverse effect being a cough in 10% of the subjects. The mean±SD cell yield was higher in bronchoscope-directed than blind brushings (5.1±2.4 versus 3.1±1.4×10 6 cells). Immunocytochemistry confirmed an epithelial cell lineage. Culture supernatant cytokine concentrations were similar regardless of sampling technique with patterns preserved between asthmatic and healthy nonatopic phenotypes. Compared with blind brushing portable bronchoscope-directed brushing is well-tolerated, yields significantly more cells and is a potentially quick and useful technique for obtaining airway epithelial cells for research into childhood respiratory disease, specifically asthma. Copyright©ERS Journals Ltd 2008. 2008 Journal Article http://hdl.handle.net/20.500.11937/76827 10.1183/09031936.00162507 English EUROPEAN RESPIRATORY SOC JOURNALS LTD restricted |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Respiratory System airway epithelial cells bronchoscopy brush biopsy child PROTECTED-SPECIMEN BRUSH CYSTIC-FIBROSIS ASTHMA PNEUMONIA SAFETY BIOPSY SIZE McNamara, P.S. Kicic, Anthony Sutanto, E.N. Stevens, P.T. Stick, S.M. Comparison of techniques for obtaining lower airway epithelial cells from children |
| title | Comparison of techniques for obtaining lower airway epithelial cells from children |
| title_full | Comparison of techniques for obtaining lower airway epithelial cells from children |
| title_fullStr | Comparison of techniques for obtaining lower airway epithelial cells from children |
| title_full_unstemmed | Comparison of techniques for obtaining lower airway epithelial cells from children |
| title_short | Comparison of techniques for obtaining lower airway epithelial cells from children |
| title_sort | comparison of techniques for obtaining lower airway epithelial cells from children |
| topic | Science & Technology Life Sciences & Biomedicine Respiratory System airway epithelial cells bronchoscopy brush biopsy child PROTECTED-SPECIMEN BRUSH CYSTIC-FIBROSIS ASTHMA PNEUMONIA SAFETY BIOPSY SIZE |
| url | http://hdl.handle.net/20.500.11937/76827 |