Characterization of side population cells from human airway epithelium
The airway epithelium is the first line of contact with the inhaled external environment and is continuously exposed to and injured by pollutants, allergens, and viruses. However, little is known about epithelial repair and in particular the identity and role of tissue resident stem/progenitor cells...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
WILEY
2008
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/76825 |
| _version_ | 1848763769927237632 |
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| author | Hackett, T.L. Shaheen, F. Johnson, A. Wadsworth, S. Pechkovsky, D.V. Jacoby, D.B. Kicic, Anthony Stick, S.M. Knight, D.A. |
| author_facet | Hackett, T.L. Shaheen, F. Johnson, A. Wadsworth, S. Pechkovsky, D.V. Jacoby, D.B. Kicic, Anthony Stick, S.M. Knight, D.A. |
| author_sort | Hackett, T.L. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The airway epithelium is the first line of contact with the inhaled external environment and is continuously exposed to and injured by pollutants, allergens, and viruses. However, little is known about epithelial repair and in particular the identity and role of tissue resident stem/progenitor cells that may contribute to epithelial regeneration. The aims of the present study were to identify, isolate, and characterize side population (SP) cells in human tracheobronchial epithelium. Epithelial cells were obtained from seven nontransplantable healthy lungs and four asthmatic lungs by pronase digestion. SP cells were identified by verapamil-sensitive efflux of the DNA-binding dye Hoechst 33342. Using flow cytometry, CD45- SP, CD45+ SP, and non-SP cells were isolated and sorted. CD45- SP cells made up 0.12% ± 0.01% of the total epithelial cell population in normal airway but 4.1% ± 0.06% of the epithelium in asthmatic airways. All CD45 - SP cells showed positive staining for epithelial-specific markers cytokeratin-5, E-cadherin, ZO-1, and p63. CD45- SP cells exhibited stable telomere length and increased colony-forming and proliferative potential, undergoing population expansion for at least 16 consecutive passages. In contrast with non-SP cells, fewer than 100 CD45- SP cells were able to generate a multilayered and differentiated epithelium in air-liquid interface culture. SP cells are present in human tracheobronchial epithelium, exhibit both short- and long-term proliferative potential, and are capable of generation of differentiated epithelium in vitro. The number of SP cells is significantly greater in asthmatic airways, providing evidence of dysregulated resident SP cells in the asthmatic epithelium. ©AlphaMed Press. |
| first_indexed | 2025-11-14T11:08:44Z |
| format | Journal Article |
| id | curtin-20.500.11937-76825 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:08:44Z |
| publishDate | 2008 |
| publisher | WILEY |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-768252019-11-12T08:18:15Z Characterization of side population cells from human airway epithelium Hackett, T.L. Shaheen, F. Johnson, A. Wadsworth, S. Pechkovsky, D.V. Jacoby, D.B. Kicic, Anthony Stick, S.M. Knight, D.A. Science & Technology Life Sciences & Biomedicine Cell & Tissue Engineering Biotechnology & Applied Microbiology Oncology Cell Biology Hematology Epithelium Tissue-specific stem cell Human Asthma STEM-CELLS FUNCTIONAL-PROPERTIES PROGENITOR CELLS IN-VITRO EXPRESSION TELOMERASE ADULT LUNG PROLIFERATION TRANSPORTER The airway epithelium is the first line of contact with the inhaled external environment and is continuously exposed to and injured by pollutants, allergens, and viruses. However, little is known about epithelial repair and in particular the identity and role of tissue resident stem/progenitor cells that may contribute to epithelial regeneration. The aims of the present study were to identify, isolate, and characterize side population (SP) cells in human tracheobronchial epithelium. Epithelial cells were obtained from seven nontransplantable healthy lungs and four asthmatic lungs by pronase digestion. SP cells were identified by verapamil-sensitive efflux of the DNA-binding dye Hoechst 33342. Using flow cytometry, CD45- SP, CD45+ SP, and non-SP cells were isolated and sorted. CD45- SP cells made up 0.12% ± 0.01% of the total epithelial cell population in normal airway but 4.1% ± 0.06% of the epithelium in asthmatic airways. All CD45 - SP cells showed positive staining for epithelial-specific markers cytokeratin-5, E-cadherin, ZO-1, and p63. CD45- SP cells exhibited stable telomere length and increased colony-forming and proliferative potential, undergoing population expansion for at least 16 consecutive passages. In contrast with non-SP cells, fewer than 100 CD45- SP cells were able to generate a multilayered and differentiated epithelium in air-liquid interface culture. SP cells are present in human tracheobronchial epithelium, exhibit both short- and long-term proliferative potential, and are capable of generation of differentiated epithelium in vitro. The number of SP cells is significantly greater in asthmatic airways, providing evidence of dysregulated resident SP cells in the asthmatic epithelium. ©AlphaMed Press. 2008 Journal Article http://hdl.handle.net/20.500.11937/76825 10.1634/stemcells.2008-0171 English WILEY restricted |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Cell & Tissue Engineering Biotechnology & Applied Microbiology Oncology Cell Biology Hematology Epithelium Tissue-specific stem cell Human Asthma STEM-CELLS FUNCTIONAL-PROPERTIES PROGENITOR CELLS IN-VITRO EXPRESSION TELOMERASE ADULT LUNG PROLIFERATION TRANSPORTER Hackett, T.L. Shaheen, F. Johnson, A. Wadsworth, S. Pechkovsky, D.V. Jacoby, D.B. Kicic, Anthony Stick, S.M. Knight, D.A. Characterization of side population cells from human airway epithelium |
| title | Characterization of side population cells from human airway epithelium |
| title_full | Characterization of side population cells from human airway epithelium |
| title_fullStr | Characterization of side population cells from human airway epithelium |
| title_full_unstemmed | Characterization of side population cells from human airway epithelium |
| title_short | Characterization of side population cells from human airway epithelium |
| title_sort | characterization of side population cells from human airway epithelium |
| topic | Science & Technology Life Sciences & Biomedicine Cell & Tissue Engineering Biotechnology & Applied Microbiology Oncology Cell Biology Hematology Epithelium Tissue-specific stem cell Human Asthma STEM-CELLS FUNCTIONAL-PROPERTIES PROGENITOR CELLS IN-VITRO EXPRESSION TELOMERASE ADULT LUNG PROLIFERATION TRANSPORTER |
| url | http://hdl.handle.net/20.500.11937/76825 |