Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus
© AlphaMed Press 2015. Airway epithelial cells generated from pluripotent stem cells (PSCs) represent a resource for research into a variety of human respiratory conditions, including those resulting from infection with common human pathogens. Using an NKX2.1-GFP reporter humanembryonic stem cell li...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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ALPHAMED PRESS
2015
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/76816 |
| _version_ | 1848763767436869632 |
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| author | Jenny, R.A. Hirst, C. Lim, S.M. Goulburn, A.L. Micallef, S.J. Labonne, T. Kicic, Anthony Ling, K.M. Stick, S.M. Ng, E.S. Trounson, A. Giudice, A. Elefanty, A.G. Stanley, E.G. |
| author_facet | Jenny, R.A. Hirst, C. Lim, S.M. Goulburn, A.L. Micallef, S.J. Labonne, T. Kicic, Anthony Ling, K.M. Stick, S.M. Ng, E.S. Trounson, A. Giudice, A. Elefanty, A.G. Stanley, E.G. |
| author_sort | Jenny, R.A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © AlphaMed Press 2015. Airway epithelial cells generated from pluripotent stem cells (PSCs) represent a resource for research into a variety of human respiratory conditions, including those resulting from infection with common human pathogens. Using an NKX2.1-GFP reporter humanembryonic stem cell line, wede-veloped a serum-free protocol for the generation of NKX2.1 < sup > + < /sup > endoderm that, when transplanted into immunodeficient mice, matured into respiratory cell types identified by expression of CC10, MUC5AC, and surfactant proteins. Gene profiling experiments indicated that day 10 NKX2.1 < sup > + < /sup > endo-derm expressed markers indicative of early foregut but lacked genes associated with later stages of respiratory epithelial cell differentiation. Nevertheless, NKX2.1 < sup > + < /sup > endoderm supported the infection and replication of the common respiratory pathogen human rhinovirus HRV1b. Moreover, NKX2.1 < sup > + < /sup > endoderm upregulated expression of IL-6, IL-8, and IL-1B in response to infection, a characteristic of human airway epithelial cells. Our experiments provide proof of principle for the use of PSC-derived respiratory epithelial cells in the study of cell-virus interactions. |
| first_indexed | 2025-11-14T11:08:42Z |
| format | Journal Article |
| id | curtin-20.500.11937-76816 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:08:42Z |
| publishDate | 2015 |
| publisher | ALPHAMED PRESS |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-768162019-11-12T06:06:08Z Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus Jenny, R.A. Hirst, C. Lim, S.M. Goulburn, A.L. Micallef, S.J. Labonne, T. Kicic, Anthony Ling, K.M. Stick, S.M. Ng, E.S. Trounson, A. Giudice, A. Elefanty, A.G. Stanley, E.G. Science & Technology Life Sciences & Biomedicine Cell & Tissue Engineering Cell Biology Human embryonic stem cells NKX2.1 Respiratory endoderm Lung HRV Differentiation Rhinovirus GFP ANTERIOR FOREGUT ENDODERM TRANSCRIPTION FACTOR-I PDGF RECEPTOR-ALPHA DIRECTED DIFFERENTIATION DEFINITIVE ENDODERM LUNG MORPHOGENESIS PRIMITIVE STREAK AIRWAY EPITHELIA CYSTIC-FIBROSIS VITRO MODEL © AlphaMed Press 2015. Airway epithelial cells generated from pluripotent stem cells (PSCs) represent a resource for research into a variety of human respiratory conditions, including those resulting from infection with common human pathogens. Using an NKX2.1-GFP reporter humanembryonic stem cell line, wede-veloped a serum-free protocol for the generation of NKX2.1 < sup > + < /sup > endoderm that, when transplanted into immunodeficient mice, matured into respiratory cell types identified by expression of CC10, MUC5AC, and surfactant proteins. Gene profiling experiments indicated that day 10 NKX2.1 < sup > + < /sup > endo-derm expressed markers indicative of early foregut but lacked genes associated with later stages of respiratory epithelial cell differentiation. Nevertheless, NKX2.1 < sup > + < /sup > endoderm supported the infection and replication of the common respiratory pathogen human rhinovirus HRV1b. Moreover, NKX2.1 < sup > + < /sup > endoderm upregulated expression of IL-6, IL-8, and IL-1B in response to infection, a characteristic of human airway epithelial cells. Our experiments provide proof of principle for the use of PSC-derived respiratory epithelial cells in the study of cell-virus interactions. 2015 Journal Article http://hdl.handle.net/20.500.11937/76816 10.5966/sctm.2014-0274 English ALPHAMED PRESS restricted |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Cell & Tissue Engineering Cell Biology Human embryonic stem cells NKX2.1 Respiratory endoderm Lung HRV Differentiation Rhinovirus GFP ANTERIOR FOREGUT ENDODERM TRANSCRIPTION FACTOR-I PDGF RECEPTOR-ALPHA DIRECTED DIFFERENTIATION DEFINITIVE ENDODERM LUNG MORPHOGENESIS PRIMITIVE STREAK AIRWAY EPITHELIA CYSTIC-FIBROSIS VITRO MODEL Jenny, R.A. Hirst, C. Lim, S.M. Goulburn, A.L. Micallef, S.J. Labonne, T. Kicic, Anthony Ling, K.M. Stick, S.M. Ng, E.S. Trounson, A. Giudice, A. Elefanty, A.G. Stanley, E.G. Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus |
| title | Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus |
| title_full | Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus |
| title_fullStr | Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus |
| title_full_unstemmed | Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus |
| title_short | Productive infection of human embryonic stem cell-derived NKX2.1+ respiratory progenitors with human rhinovirus |
| title_sort | productive infection of human embryonic stem cell-derived nkx2.1+ respiratory progenitors with human rhinovirus |
| topic | Science & Technology Life Sciences & Biomedicine Cell & Tissue Engineering Cell Biology Human embryonic stem cells NKX2.1 Respiratory endoderm Lung HRV Differentiation Rhinovirus GFP ANTERIOR FOREGUT ENDODERM TRANSCRIPTION FACTOR-I PDGF RECEPTOR-ALPHA DIRECTED DIFFERENTIATION DEFINITIVE ENDODERM LUNG MORPHOGENESIS PRIMITIVE STREAK AIRWAY EPITHELIA CYSTIC-FIBROSIS VITRO MODEL |
| url | http://hdl.handle.net/20.500.11937/76816 |