Analysis of squalene and its transformation by-products in latent fingermarks by ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry
Transformation of squalene and its by-products in latent fingermarks over time under different storage conditions (light, dark, and underwater) was examined through ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry. Complications of assessing finge...
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| Format: | Journal Article |
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2019
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| Online Access: | http://hdl.handle.net/20.500.11937/76758 |
| _version_ | 1848763752966520832 |
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| author | Dorakumbura, Buddhika Busetti, F. Lewis, Simon |
| author_facet | Dorakumbura, Buddhika Busetti, F. Lewis, Simon |
| author_sort | Dorakumbura, Buddhika |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Transformation of squalene and its by-products in latent fingermarks over time under different storage conditions (light, dark, and underwater) was examined through ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry. Complications of assessing fingermark compositional variation over time using multiple samples with varying initial compositions were elucidated and a more rational approach was successfully demonstrated. Squalene was detected in all fresh natural fingermarks deposited on non-porous surfaces and the amount ranged between 0.20 to 11.32 μg/5 fingertips. A notable difference in the transformation of squalene was observed with different storage conditions, where a dark aquatic environment accelerated degradation of squalene compared to dark but dry conditions. Squalene monohydroperoxide was extremely short-lived in natural deposits while the amount of squalene epoxide was still increasing relative to the initial amount, after ageing under dark and aquatic conditions for up to 7 days. Some oxidation by-products of cholesterol were also tentatively identified, which exhibited a growth over time against their initial concentration under any of the storage condition tested. These by-products, therefore, show potential as biomarkers for targeted visualisation of aged deposits. |
| first_indexed | 2025-11-14T11:08:28Z |
| format | Journal Article |
| id | curtin-20.500.11937-76758 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T11:08:28Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-767582021-11-08T06:10:16Z Analysis of squalene and its transformation by-products in latent fingermarks by ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry Dorakumbura, Buddhika Busetti, F. Lewis, Simon Fingermark composition Degradation Squalene By-products High resolution accurate mass Orbitrap™ mass spectrometry Transformation of squalene and its by-products in latent fingermarks over time under different storage conditions (light, dark, and underwater) was examined through ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry. Complications of assessing fingermark compositional variation over time using multiple samples with varying initial compositions were elucidated and a more rational approach was successfully demonstrated. Squalene was detected in all fresh natural fingermarks deposited on non-porous surfaces and the amount ranged between 0.20 to 11.32 μg/5 fingertips. A notable difference in the transformation of squalene was observed with different storage conditions, where a dark aquatic environment accelerated degradation of squalene compared to dark but dry conditions. Squalene monohydroperoxide was extremely short-lived in natural deposits while the amount of squalene epoxide was still increasing relative to the initial amount, after ageing under dark and aquatic conditions for up to 7 days. Some oxidation by-products of cholesterol were also tentatively identified, which exhibited a growth over time against their initial concentration under any of the storage condition tested. These by-products, therefore, show potential as biomarkers for targeted visualisation of aged deposits. 2019 Journal Article http://hdl.handle.net/20.500.11937/76758 10.1016/j.forc.2019.100193 http://creativecommons.org/licenses/by-nc-nd/4.0/ fulltext |
| spellingShingle | Fingermark composition Degradation Squalene By-products High resolution accurate mass Orbitrap™ mass spectrometry Dorakumbura, Buddhika Busetti, F. Lewis, Simon Analysis of squalene and its transformation by-products in latent fingermarks by ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry |
| title | Analysis of squalene and its transformation by-products in latent fingermarks by ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry |
| title_full | Analysis of squalene and its transformation by-products in latent fingermarks by ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry |
| title_fullStr | Analysis of squalene and its transformation by-products in latent fingermarks by ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry |
| title_full_unstemmed | Analysis of squalene and its transformation by-products in latent fingermarks by ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry |
| title_short | Analysis of squalene and its transformation by-products in latent fingermarks by ultrahigh-performance liquid chromatography-high resolution accurate mass Orbitrap™ mass spectrometry |
| title_sort | analysis of squalene and its transformation by-products in latent fingermarks by ultrahigh-performance liquid chromatography-high resolution accurate mass orbitrap™ mass spectrometry |
| topic | Fingermark composition Degradation Squalene By-products High resolution accurate mass Orbitrap™ mass spectrometry |
| url | http://hdl.handle.net/20.500.11937/76758 |