Ceramide biosynthesis and metabolism in trophoblast syncytialization
Sphingolipid mediators such as ceramide are pleiotropic regulators of cellular growth, differentiation and apoptosis. We investigated the role of ceramide biosynthesis, metabolism and actions in term human cytotrophoblasts syncytialized over 7 days in culture. Intracellular C16 ceramide levels incre...
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| Format: | Journal Article |
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Elsevier Ireland Ltd
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/7664 |
| _version_ | 1848745434924711936 |
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| author | Singh, A. Dharmarajan, Arunasalam Aye, I. Keelan, J. |
| author_facet | Singh, A. Dharmarajan, Arunasalam Aye, I. Keelan, J. |
| author_sort | Singh, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Sphingolipid mediators such as ceramide are pleiotropic regulators of cellular growth, differentiation and apoptosis. We investigated the role of ceramide biosynthesis, metabolism and actions in term human cytotrophoblasts syncytialized over 7 days in culture. Intracellular C16 ceramide levels increased modestly after 3 days in culture, then declined. Ceramidase was present at particularly high levels in syncytialized trophoblasts; inhibition of ceramidase reduced the degree of cell fusion. Exposure to short chain C8 ceramide or aSMase enhanced secretion of the differentiation marker hCG without affecting fusion or cell viability. In contrast, pharmacological inhibition of ceramidase reduced the extent of fusion. Inhibition of the ceramide-responsive JNK and PP2A pathways did not abolish the effects of ceramide, and JNK phosphorylation was unresponsive to ceramide; however, ceramide significantly inhibited phosphorylation of Akt. This study suggests that changes in ceramide biosynthesis and metabolism play a differential role in the biochemical and morphological features of trophoblast differentiation. |
| first_indexed | 2025-11-14T06:17:18Z |
| format | Journal Article |
| id | curtin-20.500.11937-7664 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:17:18Z |
| publishDate | 2012 |
| publisher | Elsevier Ireland Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-76642017-09-13T16:02:56Z Ceramide biosynthesis and metabolism in trophoblast syncytialization Singh, A. Dharmarajan, Arunasalam Aye, I. Keelan, J. Placenta Acid sphingomyelinase Sphingolipid Cell fusion Ceramide kinase Ceramidase Sphingolipid mediators such as ceramide are pleiotropic regulators of cellular growth, differentiation and apoptosis. We investigated the role of ceramide biosynthesis, metabolism and actions in term human cytotrophoblasts syncytialized over 7 days in culture. Intracellular C16 ceramide levels increased modestly after 3 days in culture, then declined. Ceramidase was present at particularly high levels in syncytialized trophoblasts; inhibition of ceramidase reduced the degree of cell fusion. Exposure to short chain C8 ceramide or aSMase enhanced secretion of the differentiation marker hCG without affecting fusion or cell viability. In contrast, pharmacological inhibition of ceramidase reduced the extent of fusion. Inhibition of the ceramide-responsive JNK and PP2A pathways did not abolish the effects of ceramide, and JNK phosphorylation was unresponsive to ceramide; however, ceramide significantly inhibited phosphorylation of Akt. This study suggests that changes in ceramide biosynthesis and metabolism play a differential role in the biochemical and morphological features of trophoblast differentiation. 2012 Journal Article http://hdl.handle.net/20.500.11937/7664 10.1016/j.mce.2012.05.009 Elsevier Ireland Ltd restricted |
| spellingShingle | Placenta Acid sphingomyelinase Sphingolipid Cell fusion Ceramide kinase Ceramidase Singh, A. Dharmarajan, Arunasalam Aye, I. Keelan, J. Ceramide biosynthesis and metabolism in trophoblast syncytialization |
| title | Ceramide biosynthesis and metabolism in trophoblast syncytialization |
| title_full | Ceramide biosynthesis and metabolism in trophoblast syncytialization |
| title_fullStr | Ceramide biosynthesis and metabolism in trophoblast syncytialization |
| title_full_unstemmed | Ceramide biosynthesis and metabolism in trophoblast syncytialization |
| title_short | Ceramide biosynthesis and metabolism in trophoblast syncytialization |
| title_sort | ceramide biosynthesis and metabolism in trophoblast syncytialization |
| topic | Placenta Acid sphingomyelinase Sphingolipid Cell fusion Ceramide kinase Ceramidase |
| url | http://hdl.handle.net/20.500.11937/7664 |