Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model
© 2019 Elsevier Inc. The resurgence of whooping cough reflects novel genetic variants of Bordetella pertussis and inadequate protection conferred by current acellular vaccines (aP). Biofilm is a source of novel vaccine candidates, including membrane protein assembly factor (BamB) and lipopolysacchar...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
Academic Press
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11937/75434 |
| _version_ | 1848763479934107648 |
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| author | Dorji, Dorji Graham, Ross Singh, Abhishek Ramsay, Joshua Price, Patricia Lee, Silvia |
| author_facet | Dorji, Dorji Graham, Ross Singh, Abhishek Ramsay, Joshua Price, Patricia Lee, Silvia |
| author_sort | Dorji, Dorji |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2019 Elsevier Inc. The resurgence of whooping cough reflects novel genetic variants of Bordetella pertussis and inadequate protection conferred by current acellular vaccines (aP). Biofilm is a source of novel vaccine candidates, including membrane protein assembly factor (BamB) and lipopolysaccharide assembly protein (LptD). Responses of BALB/c mice to candidate vaccines included IFN-γ and IL-17a production by spleen and lymph node cells, and serum IgG1 and IgG2a reactive with whole bacteria or aP. Protection was determined using bacterial cultured from lungs of vaccinated mice challenged with virulent B. pertussis. Mice vaccinated with biofilm produced efficient IFN-γ responses and more IL-17a and IgG2a than mice vaccinated with planktonic cells, aP or adjuvant alone. Vaccination with aP produced abundant IgG1 with little IgG2a. Mice vaccinated with aP plus BamB and LptD retained lower bacterial loads than mice vaccinated with aP alone. Whooping cough vaccines formulated with biofilm antigens, including BamB and LptD, may have clinical value. |
| first_indexed | 2025-11-14T11:04:07Z |
| format | Journal Article |
| id | curtin-20.500.11937-75434 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:04:07Z |
| publishDate | 2019 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-754342020-02-03T06:47:45Z Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model Dorji, Dorji Graham, Ross Singh, Abhishek Ramsay, Joshua Price, Patricia Lee, Silvia Science & Technology Life Sciences & Biomedicine Cell Biology Immunology Bordetella pertussis vaccine Biofilm Biofilm-associated proteins Whooping cough WHOLE-CELL VACCINE RESPONSES PERTACTIN STRAINS TOXIN TH1 © 2019 Elsevier Inc. The resurgence of whooping cough reflects novel genetic variants of Bordetella pertussis and inadequate protection conferred by current acellular vaccines (aP). Biofilm is a source of novel vaccine candidates, including membrane protein assembly factor (BamB) and lipopolysaccharide assembly protein (LptD). Responses of BALB/c mice to candidate vaccines included IFN-γ and IL-17a production by spleen and lymph node cells, and serum IgG1 and IgG2a reactive with whole bacteria or aP. Protection was determined using bacterial cultured from lungs of vaccinated mice challenged with virulent B. pertussis. Mice vaccinated with biofilm produced efficient IFN-γ responses and more IL-17a and IgG2a than mice vaccinated with planktonic cells, aP or adjuvant alone. Vaccination with aP produced abundant IgG1 with little IgG2a. Mice vaccinated with aP plus BamB and LptD retained lower bacterial loads than mice vaccinated with aP alone. Whooping cough vaccines formulated with biofilm antigens, including BamB and LptD, may have clinical value. 2019 Journal Article http://hdl.handle.net/20.500.11937/75434 10.1016/j.cellimm.2019.01.006 English Academic Press fulltext |
| spellingShingle | Science & Technology Life Sciences & Biomedicine Cell Biology Immunology Bordetella pertussis vaccine Biofilm Biofilm-associated proteins Whooping cough WHOLE-CELL VACCINE RESPONSES PERTACTIN STRAINS TOXIN TH1 Dorji, Dorji Graham, Ross Singh, Abhishek Ramsay, Joshua Price, Patricia Lee, Silvia Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model |
| title | Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model |
| title_full | Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model |
| title_fullStr | Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model |
| title_full_unstemmed | Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model |
| title_short | Immunogenicity and protective potential of Bordetella pertussis biofilm and its associated antigens in a murine model |
| title_sort | immunogenicity and protective potential of bordetella pertussis biofilm and its associated antigens in a murine model |
| topic | Science & Technology Life Sciences & Biomedicine Cell Biology Immunology Bordetella pertussis vaccine Biofilm Biofilm-associated proteins Whooping cough WHOLE-CELL VACCINE RESPONSES PERTACTIN STRAINS TOXIN TH1 |
| url | http://hdl.handle.net/20.500.11937/75434 |