Nucleic acid extraction from biomining microorganisms
Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Conference Paper |
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Trans Tech Publications
2009
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| Subjects: | |
| Online Access: | http://www.scientific.net http://hdl.handle.net/20.500.11937/7534 |
| _version_ | 1848745395700629504 |
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| author | Zammit, Carla Mutch, Lesley Watling, H.R. Watkin, Elizabeth |
| author2 | AMR Editors and Editorial Board |
| author_facet | AMR Editors and Editorial Board Zammit, Carla Mutch, Lesley Watling, H.R. Watkin, Elizabeth |
| author_sort | Zammit, Carla |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying RNA were analysed. The success of each method was examined spectrophotometrically, by agarose gel electrophoresis and PCR or quantitative real time PCR (qPCR). The most important step was shown to be cellular lysis, which principally impacted on thequantity of NA extracted from each strain. The quantity and quality of extracted NA was highlydependent on the method of NA precipitation. This study resulted in the development of a NA extraction method that reliably and reproducibly extracted NA from five strains of biomining microorganisms. |
| first_indexed | 2025-11-14T06:16:41Z |
| format | Conference Paper |
| id | curtin-20.500.11937-7534 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:16:41Z |
| publishDate | 2009 |
| publisher | Trans Tech Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-75342018-09-25T00:58:15Z Nucleic acid extraction from biomining microorganisms Zammit, Carla Mutch, Lesley Watling, H.R. Watkin, Elizabeth AMR Editors and Editorial Board RNA extraction DNA extraction Molecular Methods Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying RNA were analysed. The success of each method was examined spectrophotometrically, by agarose gel electrophoresis and PCR or quantitative real time PCR (qPCR). The most important step was shown to be cellular lysis, which principally impacted on thequantity of NA extracted from each strain. The quantity and quality of extracted NA was highlydependent on the method of NA precipitation. This study resulted in the development of a NA extraction method that reliably and reproducibly extracted NA from five strains of biomining microorganisms. 2009 Conference Paper http://hdl.handle.net/20.500.11937/7534 http://www.scientific.net Trans Tech Publications restricted |
| spellingShingle | RNA extraction DNA extraction Molecular Methods Zammit, Carla Mutch, Lesley Watling, H.R. Watkin, Elizabeth Nucleic acid extraction from biomining microorganisms |
| title | Nucleic acid extraction from biomining microorganisms |
| title_full | Nucleic acid extraction from biomining microorganisms |
| title_fullStr | Nucleic acid extraction from biomining microorganisms |
| title_full_unstemmed | Nucleic acid extraction from biomining microorganisms |
| title_short | Nucleic acid extraction from biomining microorganisms |
| title_sort | nucleic acid extraction from biomining microorganisms |
| topic | RNA extraction DNA extraction Molecular Methods |
| url | http://www.scientific.net http://hdl.handle.net/20.500.11937/7534 |