Epigenetic age acceleration in adolescence associates with BMI, inflammation and risk score for middle age cardiovascular disease

BACKGROUND: 'Accelerated ageing', assessed by adult DNA methylation predicts cardiovascular disease (CVD). Adolescent accelerated aging might predict CVD earlier. We investigated whether epigenetic age acceleration (assessed age 17-years) associated with adiposity/CVD-risk measured (ages 1...

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Main Authors: Huang, R., Lillycrop, K., Beilin, L., Godfrey, K., Anderson, D., Mori, T., Rauschert, S., Craig, J., Oddy, W., Ayonrinde, O., Pennell, C., Holbrook, J., Melton, Phillip
Format: Journal Article
Language:English
Published: 2019
Online Access:http://hdl.handle.net/20.500.11937/75049
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author Huang, R.
Lillycrop, K.
Beilin, L.
Godfrey, K.
Anderson, D.
Mori, T.
Rauschert, S.
Craig, J.
Oddy, W.
Ayonrinde, O.
Pennell, C.
Holbrook, J.
Melton, Phillip
author_facet Huang, R.
Lillycrop, K.
Beilin, L.
Godfrey, K.
Anderson, D.
Mori, T.
Rauschert, S.
Craig, J.
Oddy, W.
Ayonrinde, O.
Pennell, C.
Holbrook, J.
Melton, Phillip
author_sort Huang, R.
building Curtin Institutional Repository
collection Online Access
description BACKGROUND: 'Accelerated ageing', assessed by adult DNA methylation predicts cardiovascular disease (CVD). Adolescent accelerated aging might predict CVD earlier. We investigated whether epigenetic age acceleration (assessed age 17-years) associated with adiposity/CVD-risk measured (ages 17, 20, 22-years), and projected CVD by middle-age. METHODS: DNA methylation measured in peripheral blood provided 2 estimates of epigenetic age acceleration; intrinsic (IEAA, (preserved across cell types) and extrinsic (EEAA, dependent on cell admixture and methylation levels within each cell type).Adiposity was assessed by anthropometry, ultrasound and DEXA (ages 17, 20, 22 years). CVD-risk factors (lipids, HOMA-IR, blood pressure, inflammatory markers) were assessed at age 17-years. CVD development by age 47 years was calculated by Framingham algorithms. Results are presented as regression coefficients/5-year epigenetic age acceleration (IEAA/EEAA) for adiposity, CVD-risk factors and CVD development. RESULTS: In 995 participants (49.6% female, age 17.3+/-0.6 years), EEAA (/5-years) was associated with increased BMI of 2.4% (95%CI 1.2-3.6%) and 2.4% (0.8-3.9%) at 17 and 22 years, respectively. EEAA was associated with increases of 23% (3-33%) in hsCRP, 10% (4-17%) in interferon-gamma induced protein (IP-10) and 4% (2-6%) in tumour necrosis factor receptor 2 (sTNFR2), adjusted for BMI and HOMA-IR. EEAA(/5-years) results in a 4% increase in hard endpoints of CVD by 47 years old and a 3% increase, after adjustment for conventional risk factors. CONCLUSIONS: Accelerated epigenetic age in adolescence was associated with inflammation, BMI measured 5 years later, and probability of middle-age CVD. Irrespective whether this is cause or effect, assessing epigenetic age might refine disease prediction.
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spelling curtin-20.500.11937-750492020-11-27T05:59:06Z Epigenetic age acceleration in adolescence associates with BMI, inflammation and risk score for middle age cardiovascular disease Huang, R. Lillycrop, K. Beilin, L. Godfrey, K. Anderson, D. Mori, T. Rauschert, S. Craig, J. Oddy, W. Ayonrinde, O. Pennell, C. Holbrook, J. Melton, Phillip BACKGROUND: 'Accelerated ageing', assessed by adult DNA methylation predicts cardiovascular disease (CVD). Adolescent accelerated aging might predict CVD earlier. We investigated whether epigenetic age acceleration (assessed age 17-years) associated with adiposity/CVD-risk measured (ages 17, 20, 22-years), and projected CVD by middle-age. METHODS: DNA methylation measured in peripheral blood provided 2 estimates of epigenetic age acceleration; intrinsic (IEAA, (preserved across cell types) and extrinsic (EEAA, dependent on cell admixture and methylation levels within each cell type).Adiposity was assessed by anthropometry, ultrasound and DEXA (ages 17, 20, 22 years). CVD-risk factors (lipids, HOMA-IR, blood pressure, inflammatory markers) were assessed at age 17-years. CVD development by age 47 years was calculated by Framingham algorithms. Results are presented as regression coefficients/5-year epigenetic age acceleration (IEAA/EEAA) for adiposity, CVD-risk factors and CVD development. RESULTS: In 995 participants (49.6% female, age 17.3+/-0.6 years), EEAA (/5-years) was associated with increased BMI of 2.4% (95%CI 1.2-3.6%) and 2.4% (0.8-3.9%) at 17 and 22 years, respectively. EEAA was associated with increases of 23% (3-33%) in hsCRP, 10% (4-17%) in interferon-gamma induced protein (IP-10) and 4% (2-6%) in tumour necrosis factor receptor 2 (sTNFR2), adjusted for BMI and HOMA-IR. EEAA(/5-years) results in a 4% increase in hard endpoints of CVD by 47 years old and a 3% increase, after adjustment for conventional risk factors. CONCLUSIONS: Accelerated epigenetic age in adolescence was associated with inflammation, BMI measured 5 years later, and probability of middle-age CVD. Irrespective whether this is cause or effect, assessing epigenetic age might refine disease prediction. 2019 Journal Article http://hdl.handle.net/20.500.11937/75049 10.1210/jc.2018-02076 eng restricted
spellingShingle Huang, R.
Lillycrop, K.
Beilin, L.
Godfrey, K.
Anderson, D.
Mori, T.
Rauschert, S.
Craig, J.
Oddy, W.
Ayonrinde, O.
Pennell, C.
Holbrook, J.
Melton, Phillip
Epigenetic age acceleration in adolescence associates with BMI, inflammation and risk score for middle age cardiovascular disease
title Epigenetic age acceleration in adolescence associates with BMI, inflammation and risk score for middle age cardiovascular disease
title_full Epigenetic age acceleration in adolescence associates with BMI, inflammation and risk score for middle age cardiovascular disease
title_fullStr Epigenetic age acceleration in adolescence associates with BMI, inflammation and risk score for middle age cardiovascular disease
title_full_unstemmed Epigenetic age acceleration in adolescence associates with BMI, inflammation and risk score for middle age cardiovascular disease
title_short Epigenetic age acceleration in adolescence associates with BMI, inflammation and risk score for middle age cardiovascular disease
title_sort epigenetic age acceleration in adolescence associates with bmi, inflammation and risk score for middle age cardiovascular disease
url http://hdl.handle.net/20.500.11937/75049