ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastointestinal LS174T epithelial cells
The aim of this project was to show elevated P-glycoprotein (P-gp) expression decreasing bacterial association with LS174T human gastrointestinal cells, and that this effect could be reversed upon blocking functional P-gp efflux. Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa,...
| Main Authors: | , |
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| Format: | Journal Article |
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Elsevier Science
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/7488 |
| _version_ | 1848745383264518144 |
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| author | Crowe, Andrew Bebawy, M. |
| author_facet | Crowe, Andrew Bebawy, M. |
| author_sort | Crowe, Andrew |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The aim of this project was to show elevated P-glycoprotein (P-gp) expression decreasing bacterial association with LS174T human gastrointestinal cells, and that this effect could be reversed upon blocking functional P-gp efflux. Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Lactobacillus acidophilus and numerous strains of Escherichia coli, from commensal to enteropathogenic and enterohaemorrhagic strains (O157:H7) were fluorescently labelled and incubated on LS174T cultures either with or without P-gp amplification using rifampicin. PSC-833 was used as a potent functional P-gp blocking agent. Staphylococcus and Pseudomonas displayed the greatest association with the LS174T cells. Surprisingly, lactobacilli retained more fluorescence than enteropathogenic -E. coli in this system. Irrespective of attachment differences between the bacterial species, the increase in P-gp protein expression decreased bacterial fluorescence by 25–30%. This included the GFP-labelled E. coli, and enterohaemorrhagic E. coli (O157:H7). Blocking P-gp function through the co-administration of PSC-833 increased the amount of bacteria associated with P-gp expressing LS174T cells back to control levels. As most bacteria were affected to the same degree, irrespective of pathogenicity, it is unlikely that P-gp has a direct influence on adhesion of bacteria, and instead P-gp may be playing an indirect role by secreting a bank of endogenous factors or changing the local environment to one less suited to bacterial growth in general. |
| first_indexed | 2025-11-14T06:16:29Z |
| format | Journal Article |
| id | curtin-20.500.11937-7488 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:16:29Z |
| publishDate | 2012 |
| publisher | Elsevier Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-74882017-09-13T16:05:52Z ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastointestinal LS174T epithelial cells Crowe, Andrew Bebawy, M. The aim of this project was to show elevated P-glycoprotein (P-gp) expression decreasing bacterial association with LS174T human gastrointestinal cells, and that this effect could be reversed upon blocking functional P-gp efflux. Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Lactobacillus acidophilus and numerous strains of Escherichia coli, from commensal to enteropathogenic and enterohaemorrhagic strains (O157:H7) were fluorescently labelled and incubated on LS174T cultures either with or without P-gp amplification using rifampicin. PSC-833 was used as a potent functional P-gp blocking agent. Staphylococcus and Pseudomonas displayed the greatest association with the LS174T cells. Surprisingly, lactobacilli retained more fluorescence than enteropathogenic -E. coli in this system. Irrespective of attachment differences between the bacterial species, the increase in P-gp protein expression decreased bacterial fluorescence by 25–30%. This included the GFP-labelled E. coli, and enterohaemorrhagic E. coli (O157:H7). Blocking P-gp function through the co-administration of PSC-833 increased the amount of bacteria associated with P-gp expressing LS174T cells back to control levels. As most bacteria were affected to the same degree, irrespective of pathogenicity, it is unlikely that P-gp has a direct influence on adhesion of bacteria, and instead P-gp may be playing an indirect role by secreting a bank of endogenous factors or changing the local environment to one less suited to bacterial growth in general. 2012 Journal Article http://hdl.handle.net/20.500.11937/7488 10.1016/j.ejphar.2012.05.047 Elsevier Science restricted |
| spellingShingle | Crowe, Andrew Bebawy, M. ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastointestinal LS174T epithelial cells |
| title | ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastointestinal LS174T epithelial cells |
| title_full | ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastointestinal LS174T epithelial cells |
| title_fullStr | ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastointestinal LS174T epithelial cells |
| title_full_unstemmed | ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastointestinal LS174T epithelial cells |
| title_short | ABCB1 (P-glycoprotein) reduces bacterial attachment to human gastointestinal LS174T epithelial cells |
| title_sort | abcb1 (p-glycoprotein) reduces bacterial attachment to human gastointestinal ls174t epithelial cells |
| url | http://hdl.handle.net/20.500.11937/7488 |