Metabolic reprogramming of oncogene-addicted cancer cells to OXPHOS as a mechanism of drug resistance

The ability to selectively eradicate oncogene-addicted tumors while reducing systemic toxicity has endeared targeted therapies as a treatment strategy. Nevertheless, development of acquired resistance limits the benefits and durability of such a regime. Here we report evidence of enhanced reliance o...

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Main Authors: Hirpara, J., Eu, J., Tan, J., Wong, A., Clement, M., Kong, L., Ohi, N., Tsunoda, T., Qu, J., Goh, B., Pervaiz, Shazib
Format: Journal Article
Published: Elsevier BV 2019
Online Access:http://hdl.handle.net/20.500.11937/74623
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author Hirpara, J.
Eu, J.
Tan, J.
Wong, A.
Clement, M.
Kong, L.
Ohi, N.
Tsunoda, T.
Qu, J.
Goh, B.
Pervaiz, Shazib
author_facet Hirpara, J.
Eu, J.
Tan, J.
Wong, A.
Clement, M.
Kong, L.
Ohi, N.
Tsunoda, T.
Qu, J.
Goh, B.
Pervaiz, Shazib
author_sort Hirpara, J.
building Curtin Institutional Repository
collection Online Access
description The ability to selectively eradicate oncogene-addicted tumors while reducing systemic toxicity has endeared targeted therapies as a treatment strategy. Nevertheless, development of acquired resistance limits the benefits and durability of such a regime. Here we report evidence of enhanced reliance on mitochondrial oxidative phosphorylation (OXPHOS) in oncogene-addicted cancers manifesting acquired resistance to targeted therapies. To that effect, we describe a novel OXPHOS targeting activity of the small molecule compound, OPB-51602 (OPB). Of note, a priori treatment with OPB restored sensitivity to targeted therapies. Furthermore, cancer cells exhibiting stemness markers also showed selective reliance on OXPHOS and enhanced sensitivity to OPB. Importantly, in a subset of patients who developed secondary resistance to EGFR tyrosine kinase inhibitor (TKI), OPB treatment resulted in decrease in metabolic activity and reduction in tumor size. Collectively, we show here a switch to mitochondrial OXPHOS as a key driver of targeted drug resistance in oncogene-addicted cancers. This metabolic vulnerability is exploited by a novel OXPHOS inhibitor, which also shows promise in the clinical setting.
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publishDate 2019
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spelling curtin-20.500.11937-746232019-05-10T02:27:27Z Metabolic reprogramming of oncogene-addicted cancer cells to OXPHOS as a mechanism of drug resistance Hirpara, J. Eu, J. Tan, J. Wong, A. Clement, M. Kong, L. Ohi, N. Tsunoda, T. Qu, J. Goh, B. Pervaiz, Shazib The ability to selectively eradicate oncogene-addicted tumors while reducing systemic toxicity has endeared targeted therapies as a treatment strategy. Nevertheless, development of acquired resistance limits the benefits and durability of such a regime. Here we report evidence of enhanced reliance on mitochondrial oxidative phosphorylation (OXPHOS) in oncogene-addicted cancers manifesting acquired resistance to targeted therapies. To that effect, we describe a novel OXPHOS targeting activity of the small molecule compound, OPB-51602 (OPB). Of note, a priori treatment with OPB restored sensitivity to targeted therapies. Furthermore, cancer cells exhibiting stemness markers also showed selective reliance on OXPHOS and enhanced sensitivity to OPB. Importantly, in a subset of patients who developed secondary resistance to EGFR tyrosine kinase inhibitor (TKI), OPB treatment resulted in decrease in metabolic activity and reduction in tumor size. Collectively, we show here a switch to mitochondrial OXPHOS as a key driver of targeted drug resistance in oncogene-addicted cancers. This metabolic vulnerability is exploited by a novel OXPHOS inhibitor, which also shows promise in the clinical setting. 2019 Journal Article http://hdl.handle.net/20.500.11937/74623 10.1016/j.redox.2018.101076 http://creativecommons.org/licenses/by-nc-nd/4.0/ Elsevier BV fulltext
spellingShingle Hirpara, J.
Eu, J.
Tan, J.
Wong, A.
Clement, M.
Kong, L.
Ohi, N.
Tsunoda, T.
Qu, J.
Goh, B.
Pervaiz, Shazib
Metabolic reprogramming of oncogene-addicted cancer cells to OXPHOS as a mechanism of drug resistance
title Metabolic reprogramming of oncogene-addicted cancer cells to OXPHOS as a mechanism of drug resistance
title_full Metabolic reprogramming of oncogene-addicted cancer cells to OXPHOS as a mechanism of drug resistance
title_fullStr Metabolic reprogramming of oncogene-addicted cancer cells to OXPHOS as a mechanism of drug resistance
title_full_unstemmed Metabolic reprogramming of oncogene-addicted cancer cells to OXPHOS as a mechanism of drug resistance
title_short Metabolic reprogramming of oncogene-addicted cancer cells to OXPHOS as a mechanism of drug resistance
title_sort metabolic reprogramming of oncogene-addicted cancer cells to oxphos as a mechanism of drug resistance
url http://hdl.handle.net/20.500.11937/74623