Genomic and genetic insights into a cosmopolitan fungus, paecilomyces variotii (Eurotiales)
Species in the genus Paecilomyces, a member of the fungal order Eurotiales, are ubiquitous in nature and impact a variety of human endeavors. Here, the biology of one common species, Paecilomyces variotii, was explored using genomics and functional genetics. Sequencing the genome of two isolates rev...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
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Frontiers Research Foundation
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/74553 |
| _version_ | 1848763307591204864 |
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| author | Urquhart, A. Mondo, S. Mäkelä, M. Hane, James Wiebenga, A. He, G. Mihaltcheva, S. Pangilinan, J. Lipzen, A. Barry, K. De Vries, R. Grigoriev, I. Idnurm, A. |
| author_facet | Urquhart, A. Mondo, S. Mäkelä, M. Hane, James Wiebenga, A. He, G. Mihaltcheva, S. Pangilinan, J. Lipzen, A. Barry, K. De Vries, R. Grigoriev, I. Idnurm, A. |
| author_sort | Urquhart, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Species in the genus Paecilomyces, a member of the fungal order Eurotiales, are ubiquitous in nature and impact a variety of human endeavors. Here, the biology of one common species, Paecilomyces variotii, was explored using genomics and functional genetics. Sequencing the genome of two isolates revealed key genome and gene features in this species. A striking feature of the genome was the two-part nature, featuring large stretches of DNA with normal GC content separated by AT-rich regions, a hallmark of many plant-pathogenic fungal genomes. These AT-rich regions appeared to have been mutated by repeat-induced point (RIP) mutations. We developed methods for genetic transformation of P. variotii, including forward and reverse genetics as well as crossing techniques. Using transformation and crossing, RIP activity was identified, demonstrating for the first time that RIP is an active process within the order Eurotiales. A consequence of RIP is likely reflected by a reduction in numbers of genes within gene families, such as in cell wall degradation, and reflected by growth limitations on P. variotii on diverse carbon sources. Furthermore, using these transformation tools we characterized a conserved protein containing a domain of unknown function (DUF1212) and discovered it is involved in pigmentation. |
| first_indexed | 2025-11-14T11:01:23Z |
| format | Journal Article |
| id | curtin-20.500.11937-74553 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T11:01:23Z |
| publishDate | 2018 |
| publisher | Frontiers Research Foundation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-745532019-03-14T01:40:22Z Genomic and genetic insights into a cosmopolitan fungus, paecilomyces variotii (Eurotiales) Urquhart, A. Mondo, S. Mäkelä, M. Hane, James Wiebenga, A. He, G. Mihaltcheva, S. Pangilinan, J. Lipzen, A. Barry, K. De Vries, R. Grigoriev, I. Idnurm, A. Species in the genus Paecilomyces, a member of the fungal order Eurotiales, are ubiquitous in nature and impact a variety of human endeavors. Here, the biology of one common species, Paecilomyces variotii, was explored using genomics and functional genetics. Sequencing the genome of two isolates revealed key genome and gene features in this species. A striking feature of the genome was the two-part nature, featuring large stretches of DNA with normal GC content separated by AT-rich regions, a hallmark of many plant-pathogenic fungal genomes. These AT-rich regions appeared to have been mutated by repeat-induced point (RIP) mutations. We developed methods for genetic transformation of P. variotii, including forward and reverse genetics as well as crossing techniques. Using transformation and crossing, RIP activity was identified, demonstrating for the first time that RIP is an active process within the order Eurotiales. A consequence of RIP is likely reflected by a reduction in numbers of genes within gene families, such as in cell wall degradation, and reflected by growth limitations on P. variotii on diverse carbon sources. Furthermore, using these transformation tools we characterized a conserved protein containing a domain of unknown function (DUF1212) and discovered it is involved in pigmentation. 2018 Journal Article http://hdl.handle.net/20.500.11937/74553 10.3389/fmicb.2018.03058 http://creativecommons.org/licenses/by/4.0/ Frontiers Research Foundation fulltext |
| spellingShingle | Urquhart, A. Mondo, S. Mäkelä, M. Hane, James Wiebenga, A. He, G. Mihaltcheva, S. Pangilinan, J. Lipzen, A. Barry, K. De Vries, R. Grigoriev, I. Idnurm, A. Genomic and genetic insights into a cosmopolitan fungus, paecilomyces variotii (Eurotiales) |
| title | Genomic and genetic insights into a cosmopolitan fungus, paecilomyces variotii (Eurotiales) |
| title_full | Genomic and genetic insights into a cosmopolitan fungus, paecilomyces variotii (Eurotiales) |
| title_fullStr | Genomic and genetic insights into a cosmopolitan fungus, paecilomyces variotii (Eurotiales) |
| title_full_unstemmed | Genomic and genetic insights into a cosmopolitan fungus, paecilomyces variotii (Eurotiales) |
| title_short | Genomic and genetic insights into a cosmopolitan fungus, paecilomyces variotii (Eurotiales) |
| title_sort | genomic and genetic insights into a cosmopolitan fungus, paecilomyces variotii (eurotiales) |
| url | http://hdl.handle.net/20.500.11937/74553 |