Molecular characterisation of the NAM-1 genes in bread wheat in Australia
The wheat NAM-B1 and NAM-A1 genes are positively associated with grain protein content (GPC) in wheat. We conducted molecular characterisation of the NAM-1 genes in 51 Australian wheat varieties (Triticum aestivum L.), with the aim of improving GPC and nitrogen-usage efficiency in Australian wheat....
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
CSIRO Publishing
2018
|
| Online Access: | http://hdl.handle.net/20.500.11937/74148 |
| _version_ | 1848763193444270080 |
|---|---|
| author | Yang, R. Juhasz, A. Zhang, Y. Chen, X. Zhang, Y. She, M. Zhang, J. Maddern, R. Edwards, I. Diepeveen, Dean Islam, S. Ma, W. |
| author_facet | Yang, R. Juhasz, A. Zhang, Y. Chen, X. Zhang, Y. She, M. Zhang, J. Maddern, R. Edwards, I. Diepeveen, Dean Islam, S. Ma, W. |
| author_sort | Yang, R. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The wheat NAM-B1 and NAM-A1 genes are positively associated with grain protein content (GPC) in wheat. We conducted molecular characterisation of the NAM-1 genes in 51 Australian wheat varieties (Triticum aestivum L.), with the aim of improving GPC and nitrogen-usage efficiency in Australian wheat. In summary, the wild type NAM-B1 gene, which originated from Israel, was identified in two Australian wheat varieties. Five varieties contained a deletion allele, whereas the majority (43) harboured a non-functional NAM-B1 allele and one variety contained both functional and non-functional alleles. Twenty-six Australian wheat varieties contained the NAM-A1a haplotype, which was similar to its well-characterised homoeolog NAM-B1 wild type and associated with high GPC. The NAM-D1 gene in the 51 wheat varieties was also characterised, and no gene variation in the exon regions was noted; only two single-nucleotide polymorphisms in introns 1 and 2 were found among the 51 varieties. |
| first_indexed | 2025-11-14T10:59:34Z |
| format | Journal Article |
| id | curtin-20.500.11937-74148 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:59:34Z |
| publishDate | 2018 |
| publisher | CSIRO Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-741482019-07-16T08:13:43Z Molecular characterisation of the NAM-1 genes in bread wheat in Australia Yang, R. Juhasz, A. Zhang, Y. Chen, X. Zhang, Y. She, M. Zhang, J. Maddern, R. Edwards, I. Diepeveen, Dean Islam, S. Ma, W. The wheat NAM-B1 and NAM-A1 genes are positively associated with grain protein content (GPC) in wheat. We conducted molecular characterisation of the NAM-1 genes in 51 Australian wheat varieties (Triticum aestivum L.), with the aim of improving GPC and nitrogen-usage efficiency in Australian wheat. In summary, the wild type NAM-B1 gene, which originated from Israel, was identified in two Australian wheat varieties. Five varieties contained a deletion allele, whereas the majority (43) harboured a non-functional NAM-B1 allele and one variety contained both functional and non-functional alleles. Twenty-six Australian wheat varieties contained the NAM-A1a haplotype, which was similar to its well-characterised homoeolog NAM-B1 wild type and associated with high GPC. The NAM-D1 gene in the 51 wheat varieties was also characterised, and no gene variation in the exon regions was noted; only two single-nucleotide polymorphisms in introns 1 and 2 were found among the 51 varieties. 2018 Journal Article http://hdl.handle.net/20.500.11937/74148 10.1071/CP18273 CSIRO Publishing restricted |
| spellingShingle | Yang, R. Juhasz, A. Zhang, Y. Chen, X. Zhang, Y. She, M. Zhang, J. Maddern, R. Edwards, I. Diepeveen, Dean Islam, S. Ma, W. Molecular characterisation of the NAM-1 genes in bread wheat in Australia |
| title | Molecular characterisation of the NAM-1 genes in bread wheat in Australia |
| title_full | Molecular characterisation of the NAM-1 genes in bread wheat in Australia |
| title_fullStr | Molecular characterisation of the NAM-1 genes in bread wheat in Australia |
| title_full_unstemmed | Molecular characterisation of the NAM-1 genes in bread wheat in Australia |
| title_short | Molecular characterisation of the NAM-1 genes in bread wheat in Australia |
| title_sort | molecular characterisation of the nam-1 genes in bread wheat in australia |
| url | http://hdl.handle.net/20.500.11937/74148 |