Proteomics of the wheat tan spot pathogen Pyrenophora tritici-repentis 06 Biological Sciences 0607 Plant Biology

Objectives: The fungus Pyrenophora tritici-repentis is a major pathogen of wheat worldwide, causing the leaf spotting disease tan spot. To best inform approaches for plant genetic resistance, an understanding of the biology and pathogenicity mechanisms of this fungal pathogen is essential. Here, int...

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Main Authors: Moffat, Caroline, Stoll, T., Moolhuijzen, Paula
Format: Journal Article
Published: BioMed Central 2018
Online Access:http://hdl.handle.net/20.500.11937/73752
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author Moffat, Caroline
Stoll, T.
Moolhuijzen, Paula
author_facet Moffat, Caroline
Stoll, T.
Moolhuijzen, Paula
author_sort Moffat, Caroline
building Curtin Institutional Repository
collection Online Access
description Objectives: The fungus Pyrenophora tritici-repentis is a major pathogen of wheat worldwide, causing the leaf spotting disease tan spot. To best inform approaches for plant genetic resistance, an understanding of the biology and pathogenicity mechanisms of this fungal pathogen is essential. Here, intracellular and extracellular proteins of P. tritici-repentis were extracted, and peptides analysed via high-resolution mass spectrometry. Our objective was to generate a useful proteomics resource for P. tritici-repentis. A survey of proteins secreted by the pathogen into culture filtrate is especially useful, as these are likely to come in direct contact with the wheat host and may play important roles in infection/pathogenicity. The peptide data presented herein, has also been used to successfully verify and refine in silico predicted P. tritici-repentis gene annotations, through the validation of alternative splicing and reading frame shifts. Data description: The data sets presented consist of peptide spectra of the extracellular and intracellular proteomes of three P. tritici-repentis isolates. Peptide matches to translated transcripts of the North American reference isolate Pt-1C-BFP are also provided.
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institution Curtin University Malaysia
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spelling curtin-20.500.11937-737522019-03-14T01:45:46Z Proteomics of the wheat tan spot pathogen Pyrenophora tritici-repentis 06 Biological Sciences 0607 Plant Biology Moffat, Caroline Stoll, T. Moolhuijzen, Paula Objectives: The fungus Pyrenophora tritici-repentis is a major pathogen of wheat worldwide, causing the leaf spotting disease tan spot. To best inform approaches for plant genetic resistance, an understanding of the biology and pathogenicity mechanisms of this fungal pathogen is essential. Here, intracellular and extracellular proteins of P. tritici-repentis were extracted, and peptides analysed via high-resolution mass spectrometry. Our objective was to generate a useful proteomics resource for P. tritici-repentis. A survey of proteins secreted by the pathogen into culture filtrate is especially useful, as these are likely to come in direct contact with the wheat host and may play important roles in infection/pathogenicity. The peptide data presented herein, has also been used to successfully verify and refine in silico predicted P. tritici-repentis gene annotations, through the validation of alternative splicing and reading frame shifts. Data description: The data sets presented consist of peptide spectra of the extracellular and intracellular proteomes of three P. tritici-repentis isolates. Peptide matches to translated transcripts of the North American reference isolate Pt-1C-BFP are also provided. 2018 Journal Article http://hdl.handle.net/20.500.11937/73752 10.1186/s13104-018-3936-y http://creativecommons.org/licenses/by/4.0/ BioMed Central fulltext
spellingShingle Moffat, Caroline
Stoll, T.
Moolhuijzen, Paula
Proteomics of the wheat tan spot pathogen Pyrenophora tritici-repentis 06 Biological Sciences 0607 Plant Biology
title Proteomics of the wheat tan spot pathogen Pyrenophora tritici-repentis 06 Biological Sciences 0607 Plant Biology
title_full Proteomics of the wheat tan spot pathogen Pyrenophora tritici-repentis 06 Biological Sciences 0607 Plant Biology
title_fullStr Proteomics of the wheat tan spot pathogen Pyrenophora tritici-repentis 06 Biological Sciences 0607 Plant Biology
title_full_unstemmed Proteomics of the wheat tan spot pathogen Pyrenophora tritici-repentis 06 Biological Sciences 0607 Plant Biology
title_short Proteomics of the wheat tan spot pathogen Pyrenophora tritici-repentis 06 Biological Sciences 0607 Plant Biology
title_sort proteomics of the wheat tan spot pathogen pyrenophora tritici-repentis 06 biological sciences 0607 plant biology
url http://hdl.handle.net/20.500.11937/73752