Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System
This research aimed to repress target genes by modulating their expression through rewriting of aberrant epigenetic landscapes, including DNA methylation and histone post-translational modifications, utilizing epigenetic repressor domains. The epigenetic repressor domains included KRAB, DNMT3A, UHRF...
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| Format: | Thesis |
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Curtin University
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/73571 |
| _version_ | 1848763047292698624 |
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| author | Arooj, Mahira |
| author_facet | Arooj, Mahira |
| author_sort | Arooj, Mahira |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | This research aimed to repress target genes by modulating their expression through rewriting of aberrant epigenetic landscapes, including DNA methylation and histone post-translational modifications, utilizing epigenetic repressor domains. The epigenetic repressor domains included KRAB, DNMT3A, UHRF1 and CSD, which were delivered via the epigenetic editing tools ZFs, TALEs and the CRISPR/dCas9 system. Our findings suggest that a combination of multiple epigenetic repressor domains targeting epigenetic marks involved in epigenetic cross-talk significantly repressed target genes. |
| first_indexed | 2025-11-14T10:57:15Z |
| format | Thesis |
| id | curtin-20.500.11937-73571 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:57:15Z |
| publishDate | 2018 |
| publisher | Curtin University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-735712021-01-04T01:13:51Z Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System Arooj, Mahira This research aimed to repress target genes by modulating their expression through rewriting of aberrant epigenetic landscapes, including DNA methylation and histone post-translational modifications, utilizing epigenetic repressor domains. The epigenetic repressor domains included KRAB, DNMT3A, UHRF1 and CSD, which were delivered via the epigenetic editing tools ZFs, TALEs and the CRISPR/dCas9 system. Our findings suggest that a combination of multiple epigenetic repressor domains targeting epigenetic marks involved in epigenetic cross-talk significantly repressed target genes. 2018 Thesis http://hdl.handle.net/20.500.11937/73571 Curtin University fulltext |
| spellingShingle | Arooj, Mahira Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System |
| title | Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System |
| title_full | Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System |
| title_fullStr | Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System |
| title_full_unstemmed | Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System |
| title_short | Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System |
| title_sort | precision genome engineering and gene silencing using crispr/dcas9 system |
| url | http://hdl.handle.net/20.500.11937/73571 |