Application of Multivariate Curve Resolution Method in the Quantitative Monitoring Transformation of Salvianolic Acid A Using Online UV Spectroscopy and Mass Spectroscopy
This work presents an exploratory study of monitoring the transformation process of salvianolic acid A (SAA) using an online UV spectroscopic analysis system. A chemometrics approach, based on multivariate curve resolution of spectrophotometric matrix, was applied to resolve the concentration and sp...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
American Chemical Society
2012
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| Online Access: | http://hdl.handle.net/20.500.11937/7350 |
| _version_ | 1848745343814991872 |
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| author | Zheng, X. Gong, X. Li, Qin Qu, H. |
| author_facet | Zheng, X. Gong, X. Li, Qin Qu, H. |
| author_sort | Zheng, X. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | This work presents an exploratory study of monitoring the transformation process of salvianolic acid A (SAA) using an online UV spectroscopic analysis system. A chemometrics approach, based on multivariate curve resolution of spectrophotometric matrix, was applied to resolve the concentration and spectra profiles of reactant species and to evaluate the kinetic profile. Kinetic runs have been developed at temperatures ranging from 25 to 90 °C and pH values ranging from 2.5 to 10 in order to investigate the effects of these two variables during the transformation process of SAA. The degradation reaction of SAA was considered as first-order and the degradation kinetic constant of SAA increases when temperature increases. It was also found that SAA transforms rapidly when pH is higher than 7, but remains stable when pH is lower than 6.5. Two transformation products were auxiliarily identified by direct mass spectrometric analysis using Mass Work software. Our results suggest that the manufacturing process of Chinese medicine preparations with SAA as the main bioactive compound should not be operated at high temperature and high pH. |
| first_indexed | 2025-11-14T06:15:51Z |
| format | Journal Article |
| id | curtin-20.500.11937-7350 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T06:15:51Z |
| publishDate | 2012 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-73502017-09-13T14:33:46Z Application of Multivariate Curve Resolution Method in the Quantitative Monitoring Transformation of Salvianolic Acid A Using Online UV Spectroscopy and Mass Spectroscopy Zheng, X. Gong, X. Li, Qin Qu, H. This work presents an exploratory study of monitoring the transformation process of salvianolic acid A (SAA) using an online UV spectroscopic analysis system. A chemometrics approach, based on multivariate curve resolution of spectrophotometric matrix, was applied to resolve the concentration and spectra profiles of reactant species and to evaluate the kinetic profile. Kinetic runs have been developed at temperatures ranging from 25 to 90 °C and pH values ranging from 2.5 to 10 in order to investigate the effects of these two variables during the transformation process of SAA. The degradation reaction of SAA was considered as first-order and the degradation kinetic constant of SAA increases when temperature increases. It was also found that SAA transforms rapidly when pH is higher than 7, but remains stable when pH is lower than 6.5. Two transformation products were auxiliarily identified by direct mass spectrometric analysis using Mass Work software. Our results suggest that the manufacturing process of Chinese medicine preparations with SAA as the main bioactive compound should not be operated at high temperature and high pH. 2012 Journal Article http://hdl.handle.net/20.500.11937/7350 10.1021/ie201536y American Chemical Society restricted |
| spellingShingle | Zheng, X. Gong, X. Li, Qin Qu, H. Application of Multivariate Curve Resolution Method in the Quantitative Monitoring Transformation of Salvianolic Acid A Using Online UV Spectroscopy and Mass Spectroscopy |
| title | Application of Multivariate Curve Resolution Method in the Quantitative Monitoring Transformation of Salvianolic Acid A Using Online UV Spectroscopy and Mass Spectroscopy |
| title_full | Application of Multivariate Curve Resolution Method in the Quantitative Monitoring Transformation of Salvianolic Acid A Using Online UV Spectroscopy and Mass Spectroscopy |
| title_fullStr | Application of Multivariate Curve Resolution Method in the Quantitative Monitoring Transformation of Salvianolic Acid A Using Online UV Spectroscopy and Mass Spectroscopy |
| title_full_unstemmed | Application of Multivariate Curve Resolution Method in the Quantitative Monitoring Transformation of Salvianolic Acid A Using Online UV Spectroscopy and Mass Spectroscopy |
| title_short | Application of Multivariate Curve Resolution Method in the Quantitative Monitoring Transformation of Salvianolic Acid A Using Online UV Spectroscopy and Mass Spectroscopy |
| title_sort | application of multivariate curve resolution method in the quantitative monitoring transformation of salvianolic acid a using online uv spectroscopy and mass spectroscopy |
| url | http://hdl.handle.net/20.500.11937/7350 |