Structure-informed detection and quantification of peptides in food and biological fluids
© 2017 Wiley Periodicals, Inc. Peptides with biological properties, that is, bioactive peptides, are a class of biomolecules whose health-promoting properties are increasingly being exploited in food and health products. However, research on targeted techniques for the detection and quantification o...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Wiley-Blackwell Publishing
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/73238 |
| _version_ | 1848762962004672512 |
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| author | Agyei, D. Pan, S. Acquah, C. Bekhit, A. Danquah, Michael |
| author_facet | Agyei, D. Pan, S. Acquah, C. Bekhit, A. Danquah, Michael |
| author_sort | Agyei, D. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2017 Wiley Periodicals, Inc. Peptides with biological properties, that is, bioactive peptides, are a class of biomolecules whose health-promoting properties are increasingly being exploited in food and health products. However, research on targeted techniques for the detection and quantification of these peptides is still in its infancy. Such information is needed in order to enhance the biological and chemometric characterization of peptides and their subsequent application in the functional food and pharmaceutical industries. In this review, the role of classic techniques such as electrophoretic, chromatographic, and peptide mass spectrometry in the structure-informed detection and quantitation of bioactive peptides are discussed. Prospects for the use of aptamers in the characterization of bioactive peptides are also discussed. Practical applications: Although bioactive peptides have huge potential applications in the functional foods and health area, there are limited techniques in enhancing throughput detection, quantification, and characterization of these peptides. This review discusses state-of-the-art techniques relevant in complementing bioactive detection and profiling irrespective of the small number of amino acid units. Insights into challenges, possible remedies and prevailing areas requiring thorough research in the extant literature for food chemists and biotechnologists are also presented. |
| first_indexed | 2025-11-14T10:55:53Z |
| format | Journal Article |
| id | curtin-20.500.11937-73238 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:55:53Z |
| publishDate | 2017 |
| publisher | Wiley-Blackwell Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-732382018-12-13T09:35:41Z Structure-informed detection and quantification of peptides in food and biological fluids Agyei, D. Pan, S. Acquah, C. Bekhit, A. Danquah, Michael © 2017 Wiley Periodicals, Inc. Peptides with biological properties, that is, bioactive peptides, are a class of biomolecules whose health-promoting properties are increasingly being exploited in food and health products. However, research on targeted techniques for the detection and quantification of these peptides is still in its infancy. Such information is needed in order to enhance the biological and chemometric characterization of peptides and their subsequent application in the functional food and pharmaceutical industries. In this review, the role of classic techniques such as electrophoretic, chromatographic, and peptide mass spectrometry in the structure-informed detection and quantitation of bioactive peptides are discussed. Prospects for the use of aptamers in the characterization of bioactive peptides are also discussed. Practical applications: Although bioactive peptides have huge potential applications in the functional foods and health area, there are limited techniques in enhancing throughput detection, quantification, and characterization of these peptides. This review discusses state-of-the-art techniques relevant in complementing bioactive detection and profiling irrespective of the small number of amino acid units. Insights into challenges, possible remedies and prevailing areas requiring thorough research in the extant literature for food chemists and biotechnologists are also presented. 2017 Journal Article http://hdl.handle.net/20.500.11937/73238 10.1111/jfbc.12482 Wiley-Blackwell Publishing restricted |
| spellingShingle | Agyei, D. Pan, S. Acquah, C. Bekhit, A. Danquah, Michael Structure-informed detection and quantification of peptides in food and biological fluids |
| title | Structure-informed detection and quantification of peptides in food and biological fluids |
| title_full | Structure-informed detection and quantification of peptides in food and biological fluids |
| title_fullStr | Structure-informed detection and quantification of peptides in food and biological fluids |
| title_full_unstemmed | Structure-informed detection and quantification of peptides in food and biological fluids |
| title_short | Structure-informed detection and quantification of peptides in food and biological fluids |
| title_sort | structure-informed detection and quantification of peptides in food and biological fluids |
| url | http://hdl.handle.net/20.500.11937/73238 |