Visualization and Modeling of the In Vivo Distribution of Mesenchymal Stem Cells

Copyright © 2017 John Wiley & Sons, Inc. This unit describes a protocol for elucidating the in vivo disposition of administered mesenchymal stem cells (MSCs). Specifically, direct visualization of donor cell spatiotemporal distribution and assessment of donor cell quantity in recipient organs...

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Main Authors: Wang, H., Thorling, C., Xu, Z., Crawford, D., Liang, X., Liu, Jian, Roberts, M.
Format: Journal Article
Published: 2017
Online Access:http://hdl.handle.net/20.500.11937/73114
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author Wang, H.
Thorling, C.
Xu, Z.
Crawford, D.
Liang, X.
Liu, Jian
Roberts, M.
author_facet Wang, H.
Thorling, C.
Xu, Z.
Crawford, D.
Liang, X.
Liu, Jian
Roberts, M.
author_sort Wang, H.
building Curtin Institutional Repository
collection Online Access
description Copyright © 2017 John Wiley & Sons, Inc. This unit describes a protocol for elucidating the in vivo disposition of administered mesenchymal stem cells (MSCs). Specifically, direct visualization of donor cell spatiotemporal distribution and assessment of donor cell quantity in recipient organs are described. Protocols for data analysis are suggested, with the goal of developing a model to characterize and predict the physiological kinetics of administered MSCs. The use of this model is described, suggesting that it can be applied to abnormal conditions and has potential interspecies and inter-route predictive capability. These universal methods can be employed, regardless of the type of stem cell or disease, to guide future experiments and design treatment protocols. © 2017 by John Wiley & Sons, Inc.
first_indexed 2025-11-14T10:55:20Z
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institution Curtin University Malaysia
institution_category Local University
last_indexed 2025-11-14T10:55:20Z
publishDate 2017
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spelling curtin-20.500.11937-731142018-12-13T09:35:22Z Visualization and Modeling of the In Vivo Distribution of Mesenchymal Stem Cells Wang, H. Thorling, C. Xu, Z. Crawford, D. Liang, X. Liu, Jian Roberts, M. Copyright © 2017 John Wiley & Sons, Inc. This unit describes a protocol for elucidating the in vivo disposition of administered mesenchymal stem cells (MSCs). Specifically, direct visualization of donor cell spatiotemporal distribution and assessment of donor cell quantity in recipient organs are described. Protocols for data analysis are suggested, with the goal of developing a model to characterize and predict the physiological kinetics of administered MSCs. The use of this model is described, suggesting that it can be applied to abnormal conditions and has potential interspecies and inter-route predictive capability. These universal methods can be employed, regardless of the type of stem cell or disease, to guide future experiments and design treatment protocols. © 2017 by John Wiley & Sons, Inc. 2017 Journal Article http://hdl.handle.net/20.500.11937/73114 10.1002/cpsc.39 restricted
spellingShingle Wang, H.
Thorling, C.
Xu, Z.
Crawford, D.
Liang, X.
Liu, Jian
Roberts, M.
Visualization and Modeling of the In Vivo Distribution of Mesenchymal Stem Cells
title Visualization and Modeling of the In Vivo Distribution of Mesenchymal Stem Cells
title_full Visualization and Modeling of the In Vivo Distribution of Mesenchymal Stem Cells
title_fullStr Visualization and Modeling of the In Vivo Distribution of Mesenchymal Stem Cells
title_full_unstemmed Visualization and Modeling of the In Vivo Distribution of Mesenchymal Stem Cells
title_short Visualization and Modeling of the In Vivo Distribution of Mesenchymal Stem Cells
title_sort visualization and modeling of the in vivo distribution of mesenchymal stem cells
url http://hdl.handle.net/20.500.11937/73114