Lipid profiles of prostate cancer cells
Lipids are important cellular components which can be significantly altered in a range of disease states including prostate cancer. Here, a unique systematic approach has been used to define lipid profiles of prostate cancer cell lines, using quantitative mass spectrometry (LC-ESI-MS/MS), FTIR spect...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
Impact Journals LLC
2018
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| Online Access: | http://purl.org/au-research/grants/arc/FT130100033 http://hdl.handle.net/20.500.11937/73025 |
| _version_ | 1848762904077139968 |
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| author | Sorvina, A. Bader, C. Caporale, C. Carter, E. Johnson, I. Parkinson-Lawrence, E. Simpson, Peter Wright, P. Stagni, S. Lay, P. Massi, Massimiliano Brooks, D. Plush, S. |
| author_facet | Sorvina, A. Bader, C. Caporale, C. Carter, E. Johnson, I. Parkinson-Lawrence, E. Simpson, Peter Wright, P. Stagni, S. Lay, P. Massi, Massimiliano Brooks, D. Plush, S. |
| author_sort | Sorvina, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Lipids are important cellular components which can be significantly altered in a range of disease states including prostate cancer. Here, a unique systematic approach has been used to define lipid profiles of prostate cancer cell lines, using quantitative mass spectrometry (LC-ESI-MS/MS), FTIR spectroscopy and fluorescent microscopy. All three approaches identified significant difference in the lipid profiles of the three prostate cancer cell lines (DU145, LNCaP and 22RV1) and one non-malignant cell line (PNT1a). Specific lipid classes and species, such as phospholipids (e.g., phosphatidylethanolamine 18:1/16:0 and 18:1/18:1) and cholesteryl esters, detected by LC-ESI-MS/MS, allowed statistical separation of all four prostate cell lines. Lipid mapping by FTIR revealed that variations in these lipid classes could also be detected at a single cell level, however further investigation into this approach would be needed to generate large enough data sets for quantitation. Visualisation by fluorescence microscopy showed striking variations that could be observed in lipid staining patterns between cell lines allowing visual separation of cell lines. In particular, polar lipid staining by a fluorescent marker was observed to increase significantly in prostate cancer lines cells, when compared to PNT1a cells, which was consistent with lipid quantitation by LC-ESI-MS/MS and FTIR spectroscopy. Thus, multiple technologies can be employed to either quantify or visualise changes in lipid composition, and moreover specific lipid profiles could be used to detect and phenotype prostate cancer cells. |
| first_indexed | 2025-11-14T10:54:58Z |
| format | Journal Article |
| id | curtin-20.500.11937-73025 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:54:58Z |
| publishDate | 2018 |
| publisher | Impact Journals LLC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-730252022-11-23T07:32:34Z Lipid profiles of prostate cancer cells Sorvina, A. Bader, C. Caporale, C. Carter, E. Johnson, I. Parkinson-Lawrence, E. Simpson, Peter Wright, P. Stagni, S. Lay, P. Massi, Massimiliano Brooks, D. Plush, S. Lipids are important cellular components which can be significantly altered in a range of disease states including prostate cancer. Here, a unique systematic approach has been used to define lipid profiles of prostate cancer cell lines, using quantitative mass spectrometry (LC-ESI-MS/MS), FTIR spectroscopy and fluorescent microscopy. All three approaches identified significant difference in the lipid profiles of the three prostate cancer cell lines (DU145, LNCaP and 22RV1) and one non-malignant cell line (PNT1a). Specific lipid classes and species, such as phospholipids (e.g., phosphatidylethanolamine 18:1/16:0 and 18:1/18:1) and cholesteryl esters, detected by LC-ESI-MS/MS, allowed statistical separation of all four prostate cell lines. Lipid mapping by FTIR revealed that variations in these lipid classes could also be detected at a single cell level, however further investigation into this approach would be needed to generate large enough data sets for quantitation. Visualisation by fluorescence microscopy showed striking variations that could be observed in lipid staining patterns between cell lines allowing visual separation of cell lines. In particular, polar lipid staining by a fluorescent marker was observed to increase significantly in prostate cancer lines cells, when compared to PNT1a cells, which was consistent with lipid quantitation by LC-ESI-MS/MS and FTIR spectroscopy. Thus, multiple technologies can be employed to either quantify or visualise changes in lipid composition, and moreover specific lipid profiles could be used to detect and phenotype prostate cancer cells. 2018 Journal Article http://hdl.handle.net/20.500.11937/73025 10.18632/oncotarget.26222 http://purl.org/au-research/grants/arc/FT130100033 http://creativecommons.org/licenses/by/3.0/ Impact Journals LLC fulltext |
| spellingShingle | Sorvina, A. Bader, C. Caporale, C. Carter, E. Johnson, I. Parkinson-Lawrence, E. Simpson, Peter Wright, P. Stagni, S. Lay, P. Massi, Massimiliano Brooks, D. Plush, S. Lipid profiles of prostate cancer cells |
| title | Lipid profiles of prostate cancer cells |
| title_full | Lipid profiles of prostate cancer cells |
| title_fullStr | Lipid profiles of prostate cancer cells |
| title_full_unstemmed | Lipid profiles of prostate cancer cells |
| title_short | Lipid profiles of prostate cancer cells |
| title_sort | lipid profiles of prostate cancer cells |
| url | http://purl.org/au-research/grants/arc/FT130100033 http://hdl.handle.net/20.500.11937/73025 |