Influence of antenatal glucocorticoid on preterm lamb diaphragm
BackgroundPregnant women at a high risk of preterm delivery receive glucocorticoids to accelerate fetal lung maturation and surfactant synthesis. However, the effect of antenatal steroids on the developing diaphragm remains unclear. We hypothesized that maternal betamethasone impairs the fetal diaph...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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Wolters Kluwer Health
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/72847 |
| _version_ | 1848762856898560000 |
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| author | Mahzabin, T. Pillow, J. Pinniger, G. Bakker, A. Noble, P. White, R. Karisnan, K. Song, Yong |
| author_facet | Mahzabin, T. Pillow, J. Pinniger, G. Bakker, A. Noble, P. White, R. Karisnan, K. Song, Yong |
| author_sort | Mahzabin, T. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | BackgroundPregnant women at a high risk of preterm delivery receive glucocorticoids to accelerate fetal lung maturation and surfactant synthesis. However, the effect of antenatal steroids on the developing diaphragm remains unclear. We hypothesized that maternal betamethasone impairs the fetal diaphragm, and the magnitude of the detrimental effect increases with longer duration of exposure. We aimed to determine how different durations of fetal exposure to maternal betamethasone treatment influence the fetal diaphragm at the functional and molecular levels.MethodsDate-mated merino ewes received intramuscular injections of saline (control) or two doses of betamethasone (5.7 mg) at an interval of 24 h commencing either 2 or 14 days before delivery. Preterm lambs were killed after cesarean delivery at 121-day gestational age. In vitro contractile measurements were performed on the right hemidiaphragm, whereas molecular/cellular analyses used the left costal diaphragm.ResultsDifferent durations of fetal exposure to maternal betamethasone had no consistent effect on the protein metabolic pathway, expression of glucocorticoid receptor and its target genes, cellular oxidative status, or contractile properties of the fetal lamb diaphragm.ConclusionThese data suggest that the potential benefits of betamethasone exposure on preterm respiratory function are not compromised by impaired diaphragm function after low-dose maternal intramuscular glucocorticoid exposure. |
| first_indexed | 2025-11-14T10:54:13Z |
| format | Journal Article |
| id | curtin-20.500.11937-72847 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:54:13Z |
| publishDate | 2017 |
| publisher | Wolters Kluwer Health |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-728472018-12-13T09:35:13Z Influence of antenatal glucocorticoid on preterm lamb diaphragm Mahzabin, T. Pillow, J. Pinniger, G. Bakker, A. Noble, P. White, R. Karisnan, K. Song, Yong BackgroundPregnant women at a high risk of preterm delivery receive glucocorticoids to accelerate fetal lung maturation and surfactant synthesis. However, the effect of antenatal steroids on the developing diaphragm remains unclear. We hypothesized that maternal betamethasone impairs the fetal diaphragm, and the magnitude of the detrimental effect increases with longer duration of exposure. We aimed to determine how different durations of fetal exposure to maternal betamethasone treatment influence the fetal diaphragm at the functional and molecular levels.MethodsDate-mated merino ewes received intramuscular injections of saline (control) or two doses of betamethasone (5.7 mg) at an interval of 24 h commencing either 2 or 14 days before delivery. Preterm lambs were killed after cesarean delivery at 121-day gestational age. In vitro contractile measurements were performed on the right hemidiaphragm, whereas molecular/cellular analyses used the left costal diaphragm.ResultsDifferent durations of fetal exposure to maternal betamethasone had no consistent effect on the protein metabolic pathway, expression of glucocorticoid receptor and its target genes, cellular oxidative status, or contractile properties of the fetal lamb diaphragm.ConclusionThese data suggest that the potential benefits of betamethasone exposure on preterm respiratory function are not compromised by impaired diaphragm function after low-dose maternal intramuscular glucocorticoid exposure. 2017 Journal Article http://hdl.handle.net/20.500.11937/72847 10.1038/pr.2017.99 Wolters Kluwer Health restricted |
| spellingShingle | Mahzabin, T. Pillow, J. Pinniger, G. Bakker, A. Noble, P. White, R. Karisnan, K. Song, Yong Influence of antenatal glucocorticoid on preterm lamb diaphragm |
| title | Influence of antenatal glucocorticoid on preterm lamb diaphragm |
| title_full | Influence of antenatal glucocorticoid on preterm lamb diaphragm |
| title_fullStr | Influence of antenatal glucocorticoid on preterm lamb diaphragm |
| title_full_unstemmed | Influence of antenatal glucocorticoid on preterm lamb diaphragm |
| title_short | Influence of antenatal glucocorticoid on preterm lamb diaphragm |
| title_sort | influence of antenatal glucocorticoid on preterm lamb diaphragm |
| url | http://hdl.handle.net/20.500.11937/72847 |