High-throughput physiological phenotyping and screening system for the characterization of plant–environment interactions
© 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd We present a simple and effective high-throughput experimental platform for simultaneous and continuous monitoring of water relations in the soil–plant–atmosphere continuum of numerous plants under dynamic environmental conditio...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Published: |
Wiley-Blackwell Publishing Ltd.
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/72641 |
| _version_ | 1848762804407894016 |
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| author | Halperin, O. Gebremedhin, Amanuel Tesfay Wallach, R. Moshelion, M. |
| author_facet | Halperin, O. Gebremedhin, Amanuel Tesfay Wallach, R. Moshelion, M. |
| author_sort | Halperin, O. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd We present a simple and effective high-throughput experimental platform for simultaneous and continuous monitoring of water relations in the soil–plant–atmosphere continuum of numerous plants under dynamic environmental conditions. This system provides a simultaneously measured, detailed physiological response profile for each plant in the array, over time periods ranging from a few minutes to the entire growing season, under normal, stress and recovery conditions and at any phenological stage. Three probes for each pot in the array and a specially designed algorithm enable detailed water-relations characterization of whole-plant transpiration, biomass gain, stomatal conductance and root flux. They also enable quantitative calculation of the whole plant water-use efficiency and relative water content at high resolution under dynamic soil and atmospheric conditions. The system has no moving parts and can fit into many growing environments. A screening of 65 introgression lines of a wild tomato species (Solanum pennellii) crossed with cultivated tomato (S. lycopersicum), using our system and conventional gas-exchange tools, confirmed the accuracy of the system as well as its diagnostic capabilities. The use of this high-throughput diagnostic screening method is discussed in light of the gaps in our understanding of the genetic regulation of whole-plant performance, particularly under abiotic stress. |
| first_indexed | 2025-11-14T10:53:23Z |
| format | Journal Article |
| id | curtin-20.500.11937-72641 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:53:23Z |
| publishDate | 2017 |
| publisher | Wiley-Blackwell Publishing Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-726412018-12-13T09:32:29Z High-throughput physiological phenotyping and screening system for the characterization of plant–environment interactions Halperin, O. Gebremedhin, Amanuel Tesfay Wallach, R. Moshelion, M. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd We present a simple and effective high-throughput experimental platform for simultaneous and continuous monitoring of water relations in the soil–plant–atmosphere continuum of numerous plants under dynamic environmental conditions. This system provides a simultaneously measured, detailed physiological response profile for each plant in the array, over time periods ranging from a few minutes to the entire growing season, under normal, stress and recovery conditions and at any phenological stage. Three probes for each pot in the array and a specially designed algorithm enable detailed water-relations characterization of whole-plant transpiration, biomass gain, stomatal conductance and root flux. They also enable quantitative calculation of the whole plant water-use efficiency and relative water content at high resolution under dynamic soil and atmospheric conditions. The system has no moving parts and can fit into many growing environments. A screening of 65 introgression lines of a wild tomato species (Solanum pennellii) crossed with cultivated tomato (S. lycopersicum), using our system and conventional gas-exchange tools, confirmed the accuracy of the system as well as its diagnostic capabilities. The use of this high-throughput diagnostic screening method is discussed in light of the gaps in our understanding of the genetic regulation of whole-plant performance, particularly under abiotic stress. 2017 Journal Article http://hdl.handle.net/20.500.11937/72641 10.1111/tpj.13425 Wiley-Blackwell Publishing Ltd. restricted |
| spellingShingle | Halperin, O. Gebremedhin, Amanuel Tesfay Wallach, R. Moshelion, M. High-throughput physiological phenotyping and screening system for the characterization of plant–environment interactions |
| title | High-throughput physiological phenotyping and screening system for the characterization of plant–environment interactions |
| title_full | High-throughput physiological phenotyping and screening system for the characterization of plant–environment interactions |
| title_fullStr | High-throughput physiological phenotyping and screening system for the characterization of plant–environment interactions |
| title_full_unstemmed | High-throughput physiological phenotyping and screening system for the characterization of plant–environment interactions |
| title_short | High-throughput physiological phenotyping and screening system for the characterization of plant–environment interactions |
| title_sort | high-throughput physiological phenotyping and screening system for the characterization of plant–environment interactions |
| url | http://hdl.handle.net/20.500.11937/72641 |