Short-And long-Term tracking of anionic ultrasmall nanoparticles in kidney
© 2016 American Chemical Society. While biodistribution of nanoparticles (NPs) has been widely studied at the organ level, relatively little is known about their disposition in organs at the cellular level, especially after long-Term exposure. The kidney is regarded as the key organ for the clearanc...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
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American Chemical Society
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/72553 |
| _version_ | 1848762780417523712 |
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| author | Liang, X. Wang, H. Zhu, Y. Zhang, R. Cogger, V. Liu, Jian Xu, Z. Grice, J. Roberts, M. |
| author_facet | Liang, X. Wang, H. Zhu, Y. Zhang, R. Cogger, V. Liu, Jian Xu, Z. Grice, J. Roberts, M. |
| author_sort | Liang, X. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 American Chemical Society. While biodistribution of nanoparticles (NPs) has been widely studied at the organ level, relatively little is known about their disposition in organs at the cellular level, especially after long-Term exposure. The kidney is regarded as the key organ for the clearance of ultrasmall NPs (<5.5 nm). However, recent studies indicate that NPs in this size range could accumulate in the kidney for extended times without urinary excretion. Using negatively charged quantum dots (QDs) (3.7 nm) as a model system, we examined the suborgan disposition of anionic ultrasmall NPs in the kidney at the cellular level after intravenous injection by multiphoton microscopy coupled with fluorescence lifetime imaging. Most of the NPs were initially distributed in the peritubular capillaries or glomerular arterioles after injection, whereas they passed through the fenestrated glomerular endothelium and were gradually taken up by mesangial cells up to 30 days after injection. Only trace amounts of anionic QDs could be detected in the urine, which could be attributed to the barrier of the anionic glomerular basement membrane preventing filtration of anionic QDs. In contrast, cationic QDs of similar size (5.67 nm) were found to be readily excreted into urine. This study thus highlights the importance of surface charge in determining renal clearance of ultrasmall NPs. It provides a framework for characterizing and predicting the subcellular disposition in organs and long-Term targeting of other NPs, with a physiologically based kinetic model being subsequently developed to describe the suborgan kinetics of anionic ultrasmall NPs. |
| first_indexed | 2025-11-14T10:53:00Z |
| format | Journal Article |
| id | curtin-20.500.11937-72553 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:53:00Z |
| publishDate | 2016 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-725532018-12-13T09:34:10Z Short-And long-Term tracking of anionic ultrasmall nanoparticles in kidney Liang, X. Wang, H. Zhu, Y. Zhang, R. Cogger, V. Liu, Jian Xu, Z. Grice, J. Roberts, M. © 2016 American Chemical Society. While biodistribution of nanoparticles (NPs) has been widely studied at the organ level, relatively little is known about their disposition in organs at the cellular level, especially after long-Term exposure. The kidney is regarded as the key organ for the clearance of ultrasmall NPs (<5.5 nm). However, recent studies indicate that NPs in this size range could accumulate in the kidney for extended times without urinary excretion. Using negatively charged quantum dots (QDs) (3.7 nm) as a model system, we examined the suborgan disposition of anionic ultrasmall NPs in the kidney at the cellular level after intravenous injection by multiphoton microscopy coupled with fluorescence lifetime imaging. Most of the NPs were initially distributed in the peritubular capillaries or glomerular arterioles after injection, whereas they passed through the fenestrated glomerular endothelium and were gradually taken up by mesangial cells up to 30 days after injection. Only trace amounts of anionic QDs could be detected in the urine, which could be attributed to the barrier of the anionic glomerular basement membrane preventing filtration of anionic QDs. In contrast, cationic QDs of similar size (5.67 nm) were found to be readily excreted into urine. This study thus highlights the importance of surface charge in determining renal clearance of ultrasmall NPs. It provides a framework for characterizing and predicting the subcellular disposition in organs and long-Term targeting of other NPs, with a physiologically based kinetic model being subsequently developed to describe the suborgan kinetics of anionic ultrasmall NPs. 2016 Journal Article http://hdl.handle.net/20.500.11937/72553 10.1021/acsnano.5b05066 American Chemical Society restricted |
| spellingShingle | Liang, X. Wang, H. Zhu, Y. Zhang, R. Cogger, V. Liu, Jian Xu, Z. Grice, J. Roberts, M. Short-And long-Term tracking of anionic ultrasmall nanoparticles in kidney |
| title | Short-And long-Term tracking of anionic ultrasmall nanoparticles in kidney |
| title_full | Short-And long-Term tracking of anionic ultrasmall nanoparticles in kidney |
| title_fullStr | Short-And long-Term tracking of anionic ultrasmall nanoparticles in kidney |
| title_full_unstemmed | Short-And long-Term tracking of anionic ultrasmall nanoparticles in kidney |
| title_short | Short-And long-Term tracking of anionic ultrasmall nanoparticles in kidney |
| title_sort | short-and long-term tracking of anionic ultrasmall nanoparticles in kidney |
| url | http://hdl.handle.net/20.500.11937/72553 |