Case study: Applying rapid flow cytometry analysis to CAPD effluent

© 2018, Multimed Inc.. All rights reserved. Peritoneal dialysis (PD) peritonitis cases require rapid clinical interventions to ensure the best possible patient outcomes. Culture-dependent microbiology tools are slow and cannot provide clinicians with evidence to guide antimicrobial prescription prac...

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Main Authors: Mulroney, K., Hall, J., McGuire, A., Inglis, T., Chakera, Aron
Format: Journal Article
Published: 2018
Online Access:http://hdl.handle.net/20.500.11937/72333
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author Mulroney, K.
Hall, J.
McGuire, A.
Inglis, T.
Chakera, Aron
author_facet Mulroney, K.
Hall, J.
McGuire, A.
Inglis, T.
Chakera, Aron
author_sort Mulroney, K.
building Curtin Institutional Repository
collection Online Access
description © 2018, Multimed Inc.. All rights reserved. Peritoneal dialysis (PD) peritonitis cases require rapid clinical interventions to ensure the best possible patient outcomes. Culture-dependent microbiology tools are slow and cannot provide clinicians with evidence to guide antimicrobial prescription practices in an appropriate time frame. Genotypic methods have met with limited success for analyzing continuous ambulatory PD effluent, with most centers still relying on culture-dependent microbiology. We present a case study in which we apply flow cytometry techniques to antibiotic-compromised effluent. We demonstrate, with supporting evidence, direct enumeration of bacterial and human immune cells, with results reported within 2 hours of receiving the clinical specimen.
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institution Curtin University Malaysia
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last_indexed 2025-11-14T10:52:05Z
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spelling curtin-20.500.11937-723332018-12-13T09:32:08Z Case study: Applying rapid flow cytometry analysis to CAPD effluent Mulroney, K. Hall, J. McGuire, A. Inglis, T. Chakera, Aron © 2018, Multimed Inc.. All rights reserved. Peritoneal dialysis (PD) peritonitis cases require rapid clinical interventions to ensure the best possible patient outcomes. Culture-dependent microbiology tools are slow and cannot provide clinicians with evidence to guide antimicrobial prescription practices in an appropriate time frame. Genotypic methods have met with limited success for analyzing continuous ambulatory PD effluent, with most centers still relying on culture-dependent microbiology. We present a case study in which we apply flow cytometry techniques to antibiotic-compromised effluent. We demonstrate, with supporting evidence, direct enumeration of bacterial and human immune cells, with results reported within 2 hours of receiving the clinical specimen. 2018 Journal Article http://hdl.handle.net/20.500.11937/72333 10.3747/pdi.2017.00231 restricted
spellingShingle Mulroney, K.
Hall, J.
McGuire, A.
Inglis, T.
Chakera, Aron
Case study: Applying rapid flow cytometry analysis to CAPD effluent
title Case study: Applying rapid flow cytometry analysis to CAPD effluent
title_full Case study: Applying rapid flow cytometry analysis to CAPD effluent
title_fullStr Case study: Applying rapid flow cytometry analysis to CAPD effluent
title_full_unstemmed Case study: Applying rapid flow cytometry analysis to CAPD effluent
title_short Case study: Applying rapid flow cytometry analysis to CAPD effluent
title_sort case study: applying rapid flow cytometry analysis to capd effluent
url http://hdl.handle.net/20.500.11937/72333