Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression
© 2014, South Pacific Underwater Medicine Society. All rights reserved. Introduction: Splenic platelets have been recognized to have a greater prothrombotic potential than others platelets. We studied whether platelets released by splenic contraction could influence the severity and outcome of decom...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Published: |
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/72223 |
| _version_ | 1848762692989353984 |
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| author | Lambrechts, K. Pontier, J. Mazur, A. Buzzacott, Peter Goanvec, C. Wang, Q. Theron, M. Belhomme, M. Guerrero, F. |
| author_facet | Lambrechts, K. Pontier, J. Mazur, A. Buzzacott, Peter Goanvec, C. Wang, Q. Theron, M. Belhomme, M. Guerrero, F. |
| author_sort | Lambrechts, K. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2014, South Pacific Underwater Medicine Society. All rights reserved. Introduction: Splenic platelets have been recognized to have a greater prothrombotic potential than others platelets. We studied whether platelets released by splenic contraction could influence the severity and outcome of decompression sickness (DCS) and bubble-induced platelet activation. Methods: Sixteen, male Sprague-Dawley rats were randomly assigned to either a control or a splenectomized group. Both groups were compressed to 1,000 kPa (90 metres’ sea water) for 45 min while breathing air before staged decompression (5 min at 200 kPa, 5 min at 160 kPa and 10 min at 130 kPa). The onset time of DCS symptoms and of death were recorded during a 60-min observation period post dive. Parameters measured were platelet factor 4 (PF4) for platelet activation, thiobarbituric acid reactive substances (TBARS) for oxidative stress status and Von Willebrand factor (VWf) for endothelial activation. Results: There were no differences between the groups in DCS outcome or in PF4, TBARS and VWf concentrations. Conclusion: These results do not support that the spleen and its exchangeable platelet pool is involved in DCS pathogenesis in a rat model, invalidating the hypothesis that increased decompression-induced platelet aggregation could be influenced by splenic contraction and then play a role in DCS outcome. |
| first_indexed | 2025-11-14T10:51:37Z |
| format | Journal Article |
| id | curtin-20.500.11937-72223 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:51:37Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-722232018-12-13T09:12:43Z Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression Lambrechts, K. Pontier, J. Mazur, A. Buzzacott, Peter Goanvec, C. Wang, Q. Theron, M. Belhomme, M. Guerrero, F. © 2014, South Pacific Underwater Medicine Society. All rights reserved. Introduction: Splenic platelets have been recognized to have a greater prothrombotic potential than others platelets. We studied whether platelets released by splenic contraction could influence the severity and outcome of decompression sickness (DCS) and bubble-induced platelet activation. Methods: Sixteen, male Sprague-Dawley rats were randomly assigned to either a control or a splenectomized group. Both groups were compressed to 1,000 kPa (90 metres’ sea water) for 45 min while breathing air before staged decompression (5 min at 200 kPa, 5 min at 160 kPa and 10 min at 130 kPa). The onset time of DCS symptoms and of death were recorded during a 60-min observation period post dive. Parameters measured were platelet factor 4 (PF4) for platelet activation, thiobarbituric acid reactive substances (TBARS) for oxidative stress status and Von Willebrand factor (VWf) for endothelial activation. Results: There were no differences between the groups in DCS outcome or in PF4, TBARS and VWf concentrations. Conclusion: These results do not support that the spleen and its exchangeable platelet pool is involved in DCS pathogenesis in a rat model, invalidating the hypothesis that increased decompression-induced platelet aggregation could be influenced by splenic contraction and then play a role in DCS outcome. 2014 Journal Article http://hdl.handle.net/20.500.11937/72223 restricted |
| spellingShingle | Lambrechts, K. Pontier, J. Mazur, A. Buzzacott, Peter Goanvec, C. Wang, Q. Theron, M. Belhomme, M. Guerrero, F. Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression |
| title | Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression |
| title_full | Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression |
| title_fullStr | Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression |
| title_full_unstemmed | Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression |
| title_short | Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression |
| title_sort | effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression |
| url | http://hdl.handle.net/20.500.11937/72223 |