Efficient production of a mature and functional gamma secretase protease
Baculoviral protein expression in insect cells has been previously used to generate large quantities of a protein of interest for subsequent use in biochemical and structural analyses. The MultiBac baculovirus protein expression system has enabled, the use of a single baculovirus to reconstitute a p...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Published: |
Nature Publishing Group
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/72085 |
| _version_ | 1848762655592939520 |
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| author | Khan, I. Krishnaswamy, S. Sabale, M. Groth, David Wijaya, L. Morici, M. Berger, I. Schaffitzel, C. Fraser, P. Martins, R. Verdile, Giuseppe |
| author_facet | Khan, I. Krishnaswamy, S. Sabale, M. Groth, David Wijaya, L. Morici, M. Berger, I. Schaffitzel, C. Fraser, P. Martins, R. Verdile, Giuseppe |
| author_sort | Khan, I. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Baculoviral protein expression in insect cells has been previously used to generate large quantities of a protein of interest for subsequent use in biochemical and structural analyses. The MultiBac baculovirus protein expression system has enabled, the use of a single baculovirus to reconstitute a protein complex of interest, resulting in a larger protein yield. Using this system, we aimed to reconstruct the gamma (γ)-secretase complex, a multiprotein enzyme complex essential for the production of amyloid-β (Aβ) protein. A MultiBac vector containing all components of the γ-secretase complex was generated and expression was observed for all components. The complex was active in processing APP and Notch derived γ-secretase substrates and proteolysis could be inhibited with γ-secretase inhibitors, confirming specificity of the recombinant γ-secretase enzyme. Finally, affinity purification was used to purify an active recombinant γ-secretase complex. In this study we demonstrated that the MultiBac protein expression system can be used to generate an active γ-secretase complex and provides a new tool to study γ-secretase enzyme and its variants. |
| first_indexed | 2025-11-14T10:51:01Z |
| format | Journal Article |
| id | curtin-20.500.11937-72085 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:51:01Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-720852019-01-15T06:38:09Z Efficient production of a mature and functional gamma secretase protease Khan, I. Krishnaswamy, S. Sabale, M. Groth, David Wijaya, L. Morici, M. Berger, I. Schaffitzel, C. Fraser, P. Martins, R. Verdile, Giuseppe Baculoviral protein expression in insect cells has been previously used to generate large quantities of a protein of interest for subsequent use in biochemical and structural analyses. The MultiBac baculovirus protein expression system has enabled, the use of a single baculovirus to reconstitute a protein complex of interest, resulting in a larger protein yield. Using this system, we aimed to reconstruct the gamma (γ)-secretase complex, a multiprotein enzyme complex essential for the production of amyloid-β (Aβ) protein. A MultiBac vector containing all components of the γ-secretase complex was generated and expression was observed for all components. The complex was active in processing APP and Notch derived γ-secretase substrates and proteolysis could be inhibited with γ-secretase inhibitors, confirming specificity of the recombinant γ-secretase enzyme. Finally, affinity purification was used to purify an active recombinant γ-secretase complex. In this study we demonstrated that the MultiBac protein expression system can be used to generate an active γ-secretase complex and provides a new tool to study γ-secretase enzyme and its variants. 2018 Journal Article http://hdl.handle.net/20.500.11937/72085 10.1038/s41598-018-30788-w http://creativecommons.org/licenses/by/4.0/ Nature Publishing Group fulltext |
| spellingShingle | Khan, I. Krishnaswamy, S. Sabale, M. Groth, David Wijaya, L. Morici, M. Berger, I. Schaffitzel, C. Fraser, P. Martins, R. Verdile, Giuseppe Efficient production of a mature and functional gamma secretase protease |
| title | Efficient production of a mature and functional gamma secretase protease |
| title_full | Efficient production of a mature and functional gamma secretase protease |
| title_fullStr | Efficient production of a mature and functional gamma secretase protease |
| title_full_unstemmed | Efficient production of a mature and functional gamma secretase protease |
| title_short | Efficient production of a mature and functional gamma secretase protease |
| title_sort | efficient production of a mature and functional gamma secretase protease |
| url | http://hdl.handle.net/20.500.11937/72085 |