Heat-dried sclerotia of Sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect Brassica napus
The phytopathogenic fungus Sclerotinia sclerotiorum forms dormant structures (termed sclerotia) that germinate myceliogenically under certain environmental conditions. During myceliogenic germination, sclerotia produce hyphae, which can infect leaves or stems of host plants directly from the ground;...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Published: |
CSIRO Publishing
2018
|
| Online Access: | http://hdl.handle.net/20.500.11937/71997 |
| _version_ | 1848762631606763520 |
|---|---|
| author | Lane, D. Kamphuis, Lars Derbyshire, Mark Denton-Giles, Matthew |
| author_facet | Lane, D. Kamphuis, Lars Derbyshire, Mark Denton-Giles, Matthew |
| author_sort | Lane, D. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | The phytopathogenic fungus Sclerotinia sclerotiorum forms dormant structures (termed sclerotia) that germinate myceliogenically under certain environmental conditions. During myceliogenic germination, sclerotia produce hyphae, which can infect leaves or stems of host plants directly from the ground; this is termed basal infection. This study determined which abiotic conditions were most important for promoting myceliogenic germination of sclerotia in vitro. A high sclerotium hydration level and low incubation temperature (158C) improved mycelial growth in the presence of a nutrient source. Sclerotia incubated without a nutrient source on moist sand, vigorously myceliogenically germinated most frequently (63%) when they had been previously imbibed and then conditioned at -20°C. By far the most consistent amount of vigorous myceliogenic germination (>75%) was produced when sclerotia were heat-dried before being submerged in water. The hyphae of these sclerotia were shown to infect and proliferate on leaves of intact Brassica napus plants. This research provides a better understanding of the abiotic conditions that are likely to increase the risk of basal infection by S. sclerotiorum. |
| first_indexed | 2025-11-14T10:50:38Z |
| format | Journal Article |
| id | curtin-20.500.11937-71997 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:50:38Z |
| publishDate | 2018 |
| publisher | CSIRO Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-719972019-02-15T01:13:40Z Heat-dried sclerotia of Sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect Brassica napus Lane, D. Kamphuis, Lars Derbyshire, Mark Denton-Giles, Matthew The phytopathogenic fungus Sclerotinia sclerotiorum forms dormant structures (termed sclerotia) that germinate myceliogenically under certain environmental conditions. During myceliogenic germination, sclerotia produce hyphae, which can infect leaves or stems of host plants directly from the ground; this is termed basal infection. This study determined which abiotic conditions were most important for promoting myceliogenic germination of sclerotia in vitro. A high sclerotium hydration level and low incubation temperature (158C) improved mycelial growth in the presence of a nutrient source. Sclerotia incubated without a nutrient source on moist sand, vigorously myceliogenically germinated most frequently (63%) when they had been previously imbibed and then conditioned at -20°C. By far the most consistent amount of vigorous myceliogenic germination (>75%) was produced when sclerotia were heat-dried before being submerged in water. The hyphae of these sclerotia were shown to infect and proliferate on leaves of intact Brassica napus plants. This research provides a better understanding of the abiotic conditions that are likely to increase the risk of basal infection by S. sclerotiorum. 2018 Journal Article http://hdl.handle.net/20.500.11937/71997 10.1071/CP18109 http://creativecommons.org/licenses/by-nc-nd/4.0/ CSIRO Publishing fulltext |
| spellingShingle | Lane, D. Kamphuis, Lars Derbyshire, Mark Denton-Giles, Matthew Heat-dried sclerotia of Sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect Brassica napus |
| title | Heat-dried sclerotia of Sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect Brassica napus |
| title_full | Heat-dried sclerotia of Sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect Brassica napus |
| title_fullStr | Heat-dried sclerotia of Sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect Brassica napus |
| title_full_unstemmed | Heat-dried sclerotia of Sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect Brassica napus |
| title_short | Heat-dried sclerotia of Sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect Brassica napus |
| title_sort | heat-dried sclerotia of sclerotinia sclerotiorum myceliogenically germinate in water and are able to infect brassica napus |
| url | http://hdl.handle.net/20.500.11937/71997 |