Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells
Lupin seed proteins have been reported to exhibit hypoglycaemic effects in animals and humans following oral administration, however little is known about its mechanism of action. This study investigated the signalling pathway(s) responsible for the insulinotropic effect of the hydrolysate obtained...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier Ireland Ltd
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/71848 |
| _version_ | 1848762588810182656 |
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| author | Tapadia, M. Carlessi, Rodrigo Johnson, Stuart Utikar, Ranjeet Newsholme, Philip |
| author_facet | Tapadia, M. Carlessi, Rodrigo Johnson, Stuart Utikar, Ranjeet Newsholme, Philip |
| author_sort | Tapadia, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Lupin seed proteins have been reported to exhibit hypoglycaemic effects in animals and humans following oral administration, however little is known about its mechanism of action. This study investigated the signalling pathway(s) responsible for the insulinotropic effect of the hydrolysate obtained from lupin (Lupinus angustifolius L.) seed extracts utilizing BRIN-BD11 ß-cells. The extract was treated with digestive enzymes to give a hydrolysate rich in biomolecules =7 kDa. Cells exhibited hydrolysate induced dose-dependent stimulation of insulin secretion and enhanced intracellular Ca2+ and glucose metabolism. The stimulatory effect of the hydrolysate was potentiated by depolarizing concentrations of KCl and was blocked by inhibitors of the ATP sensitive K+ channel, Gaq protein, phospholipase C (PLC) and protein kinase C (PKC). These findings reveal a novel mechanism for lupin hydrolysate stimulated insulin secretion via Gaq mediated signal transduction (Gaq/PLC/PKC) in the ß-cells. Thus, lupin hydrolysates may have potential for nutraceutical treatment in type 2 diabetes. |
| first_indexed | 2025-11-14T10:49:58Z |
| format | Journal Article |
| id | curtin-20.500.11937-71848 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:49:58Z |
| publishDate | 2018 |
| publisher | Elsevier Ireland Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-718482019-02-12T03:11:45Z Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells Tapadia, M. Carlessi, Rodrigo Johnson, Stuart Utikar, Ranjeet Newsholme, Philip Lupin seed proteins have been reported to exhibit hypoglycaemic effects in animals and humans following oral administration, however little is known about its mechanism of action. This study investigated the signalling pathway(s) responsible for the insulinotropic effect of the hydrolysate obtained from lupin (Lupinus angustifolius L.) seed extracts utilizing BRIN-BD11 ß-cells. The extract was treated with digestive enzymes to give a hydrolysate rich in biomolecules =7 kDa. Cells exhibited hydrolysate induced dose-dependent stimulation of insulin secretion and enhanced intracellular Ca2+ and glucose metabolism. The stimulatory effect of the hydrolysate was potentiated by depolarizing concentrations of KCl and was blocked by inhibitors of the ATP sensitive K+ channel, Gaq protein, phospholipase C (PLC) and protein kinase C (PKC). These findings reveal a novel mechanism for lupin hydrolysate stimulated insulin secretion via Gaq mediated signal transduction (Gaq/PLC/PKC) in the ß-cells. Thus, lupin hydrolysates may have potential for nutraceutical treatment in type 2 diabetes. 2018 Journal Article http://hdl.handle.net/20.500.11937/71848 10.1016/j.mce.2018.10.015 Elsevier Ireland Ltd restricted |
| spellingShingle | Tapadia, M. Carlessi, Rodrigo Johnson, Stuart Utikar, Ranjeet Newsholme, Philip Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells |
| title | Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells |
| title_full | Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells |
| title_fullStr | Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells |
| title_full_unstemmed | Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells |
| title_short | Lupin seed hydrolysate promotes G-protein-coupled receptor, intracellular Ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from BRIN-BD11 pancreatic beta cells |
| title_sort | lupin seed hydrolysate promotes g-protein-coupled receptor, intracellular ca2+ and enhanced glycolytic metabolism-mediated insulin secretion from brin-bd11 pancreatic beta cells |
| url | http://hdl.handle.net/20.500.11937/71848 |