Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions.

The cellular and molecular microenvironment of epithelial stem/progenitor cells is critical for their long-term self-renewal. We demonstrate that mesenchymal stem cell-like dermal microvascular pericytes are a critical element of the skin's microenvironment influencing human skin regeneration u...

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Main Authors: Zhuang, L., Lawlor, K., Schlueter, H., Pieterse, Z., Yu, Y., Kaur, Pritinder
Format: Journal Article
Published: 2018
Online Access:http://hdl.handle.net/20.500.11937/71843
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author Zhuang, L.
Lawlor, K.
Schlueter, H.
Pieterse, Z.
Yu, Y.
Kaur, Pritinder
author_facet Zhuang, L.
Lawlor, K.
Schlueter, H.
Pieterse, Z.
Yu, Y.
Kaur, Pritinder
author_sort Zhuang, L.
building Curtin Institutional Repository
collection Online Access
description The cellular and molecular microenvironment of epithelial stem/progenitor cells is critical for their long-term self-renewal. We demonstrate that mesenchymal stem cell-like dermal microvascular pericytes are a critical element of the skin's microenvironment influencing human skin regeneration using organotypic models. Specifically, pericytes were capable of promoting homeostatic skin tissue renewal by conferring more planar cell divisions generating two basal cells within the proliferative compartment of the human epidermis, while ensuring complete maturation of the tissue both spatially and temporally. Moreover, we provide evidence supporting the notion that BMP-2, a secreted protein preferentially expressed by pericytes in human skin, confers cell polarity and planar divisions on epidermal cells in organotypic cultures. Our data suggest that human skin regeneration is regulated by highly conserved mechanisms at play in other rapidly renewing tissues such as the bone marrow and in lower organisms such as Drosophila. Our work also provides the means to significantly improve ex vivo skin tissue regeneration for autologous transplantation.
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spelling curtin-20.500.11937-718432021-01-08T07:54:28Z Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions. Zhuang, L. Lawlor, K. Schlueter, H. Pieterse, Z. Yu, Y. Kaur, Pritinder The cellular and molecular microenvironment of epithelial stem/progenitor cells is critical for their long-term self-renewal. We demonstrate that mesenchymal stem cell-like dermal microvascular pericytes are a critical element of the skin's microenvironment influencing human skin regeneration using organotypic models. Specifically, pericytes were capable of promoting homeostatic skin tissue renewal by conferring more planar cell divisions generating two basal cells within the proliferative compartment of the human epidermis, while ensuring complete maturation of the tissue both spatially and temporally. Moreover, we provide evidence supporting the notion that BMP-2, a secreted protein preferentially expressed by pericytes in human skin, confers cell polarity and planar divisions on epidermal cells in organotypic cultures. Our data suggest that human skin regeneration is regulated by highly conserved mechanisms at play in other rapidly renewing tissues such as the bone marrow and in lower organisms such as Drosophila. Our work also provides the means to significantly improve ex vivo skin tissue regeneration for autologous transplantation. 2018 Journal Article http://hdl.handle.net/20.500.11937/71843 10.26508/lsa.201700009 http://creativecommons.org/licenses/by/4.0/ fulltext
spellingShingle Zhuang, L.
Lawlor, K.
Schlueter, H.
Pieterse, Z.
Yu, Y.
Kaur, Pritinder
Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions.
title Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions.
title_full Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions.
title_fullStr Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions.
title_full_unstemmed Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions.
title_short Pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions.
title_sort pericytes promote skin regeneration by inducing epidermal cell polarity and planar cell divisions.
url http://hdl.handle.net/20.500.11937/71843