Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury
Oxidative stress reflects an imbalance between reactive oxygen species (ROS) and antioxidants, which has been reported as an early unifying event in the development and progression of various diseases and as a direct and mechanistic indicator of treatment response. However, highly reactive and short...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
Nature Publishing Group
2017
|
| Online Access: | http://hdl.handle.net/20.500.11937/71790 |
| _version_ | 1848762573147602944 |
|---|---|
| author | Wang, H. Zhang, R. Bridle, K. Jayachandran, A. Thomas, J. Zhang, W. Yuan, J. Xu, Z. Crawford, D. Liang, X. Liu, Jian Roberts, M. |
| author_facet | Wang, H. Zhang, R. Bridle, K. Jayachandran, A. Thomas, J. Zhang, W. Yuan, J. Xu, Z. Crawford, D. Liang, X. Liu, Jian Roberts, M. |
| author_sort | Wang, H. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Oxidative stress reflects an imbalance between reactive oxygen species (ROS) and antioxidants, which has been reported as an early unifying event in the development and progression of various diseases and as a direct and mechanistic indicator of treatment response. However, highly reactive and short-lived nature of ROS and antioxidant limited conventional detection agents, which are influenced by many interfering factors. Here, we present a two-photon sensing platform for in vivo dual imaging of oxidative stress at the single cell-level resolution. This sensing platform consists of three probes, which combine the turn-on fluorescent transition-metal complex with different specific responsive groups for glutathione (GSH), hydrogen peroxide (H2O2) and hypochlorous acid (HOCl). By combining fluorescence intensity imaging and fluorescence lifetime imaging, these probes totally remove any possibility of crosstalk from in vivo environmental or instrumental factors, and enable accurate localization and measurement of the changes in ROS and GSH within the liver. This precedes changes in conventional biochemical and histological assessments in two distinct experimental murine models of liver injury. The ability to monitor real-time cellular oxidative stress with dual-modality imaging has significant implications for high-accurate, spatially configured and quantitative assessment of metabolic status and drug response. |
| first_indexed | 2025-11-14T10:49:43Z |
| format | Journal Article |
| id | curtin-20.500.11937-71790 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:49:43Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-717902019-01-22T06:57:28Z Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury Wang, H. Zhang, R. Bridle, K. Jayachandran, A. Thomas, J. Zhang, W. Yuan, J. Xu, Z. Crawford, D. Liang, X. Liu, Jian Roberts, M. Oxidative stress reflects an imbalance between reactive oxygen species (ROS) and antioxidants, which has been reported as an early unifying event in the development and progression of various diseases and as a direct and mechanistic indicator of treatment response. However, highly reactive and short-lived nature of ROS and antioxidant limited conventional detection agents, which are influenced by many interfering factors. Here, we present a two-photon sensing platform for in vivo dual imaging of oxidative stress at the single cell-level resolution. This sensing platform consists of three probes, which combine the turn-on fluorescent transition-metal complex with different specific responsive groups for glutathione (GSH), hydrogen peroxide (H2O2) and hypochlorous acid (HOCl). By combining fluorescence intensity imaging and fluorescence lifetime imaging, these probes totally remove any possibility of crosstalk from in vivo environmental or instrumental factors, and enable accurate localization and measurement of the changes in ROS and GSH within the liver. This precedes changes in conventional biochemical and histological assessments in two distinct experimental murine models of liver injury. The ability to monitor real-time cellular oxidative stress with dual-modality imaging has significant implications for high-accurate, spatially configured and quantitative assessment of metabolic status and drug response. 2017 Journal Article http://hdl.handle.net/20.500.11937/71790 10.1038/srep45374 http://creativecommons.org/licenses/by/4.0/ Nature Publishing Group fulltext |
| spellingShingle | Wang, H. Zhang, R. Bridle, K. Jayachandran, A. Thomas, J. Zhang, W. Yuan, J. Xu, Z. Crawford, D. Liang, X. Liu, Jian Roberts, M. Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury |
| title | Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury |
| title_full | Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury |
| title_fullStr | Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury |
| title_full_unstemmed | Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury |
| title_short | Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury |
| title_sort | two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury |
| url | http://hdl.handle.net/20.500.11937/71790 |