Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization
Inorganic nanostructures and their assemblies play important roles in immobilizing biomolecules. Herein, we developed a facile and green methodology to assemble natural halloysite nanotubes (1D building blocks) into nest-like porous microspheres (3D architecture). We further modified the microsphere...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
American Chemical Society
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/71716 |
| _version_ | 1848762553684983808 |
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| author | Chao, C. Zhang, B. Zhai, R. Xiang, X. Liu, Jian Chen, R. |
| author_facet | Chao, C. Zhang, B. Zhai, R. Xiang, X. Liu, Jian Chen, R. |
| author_sort | Chao, C. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Inorganic nanostructures and their assemblies play important roles in immobilizing biomolecules. Herein, we developed a facile and green methodology to assemble natural halloysite nanotubes (1D building blocks) into nest-like porous microspheres (3D architecture). We further modified the microspheres with dopamine to form a biomimetic entity. The interconnected and hierarchical pores within the microspheres provide larger pore volume to entrap biomolecules, and the abundant functional groups on the pore surface bond covalently with enzyme to enhance the immobilization ability. The porous microspheres showed excellent loading capacity for laccase immobilization as high as 311.2 mg/g, around 30 times higher than the individual halloysite nanotubes (11.3 mg/g). The specific activity above 80% was retained for the immobilized laccase compared to the free laccase. In addition, the immobilized enzyme exhibited remarkable thermal and recycle use stability. The biomimetic microspheres are expected to be biologically safe and chemically stable microcapsules for immobilizing a variety of biomolecules because of their natural and biofriendly characteristics. © 2013 American Chemical Society. |
| first_indexed | 2025-11-14T10:49:24Z |
| format | Journal Article |
| id | curtin-20.500.11937-71716 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:49:24Z |
| publishDate | 2014 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-717162018-12-13T09:34:01Z Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization Chao, C. Zhang, B. Zhai, R. Xiang, X. Liu, Jian Chen, R. Inorganic nanostructures and their assemblies play important roles in immobilizing biomolecules. Herein, we developed a facile and green methodology to assemble natural halloysite nanotubes (1D building blocks) into nest-like porous microspheres (3D architecture). We further modified the microspheres with dopamine to form a biomimetic entity. The interconnected and hierarchical pores within the microspheres provide larger pore volume to entrap biomolecules, and the abundant functional groups on the pore surface bond covalently with enzyme to enhance the immobilization ability. The porous microspheres showed excellent loading capacity for laccase immobilization as high as 311.2 mg/g, around 30 times higher than the individual halloysite nanotubes (11.3 mg/g). The specific activity above 80% was retained for the immobilized laccase compared to the free laccase. In addition, the immobilized enzyme exhibited remarkable thermal and recycle use stability. The biomimetic microspheres are expected to be biologically safe and chemically stable microcapsules for immobilizing a variety of biomolecules because of their natural and biofriendly characteristics. © 2013 American Chemical Society. 2014 Journal Article http://hdl.handle.net/20.500.11937/71716 10.1021/sc400199v American Chemical Society restricted |
| spellingShingle | Chao, C. Zhang, B. Zhai, R. Xiang, X. Liu, Jian Chen, R. Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization |
| title | Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization |
| title_full | Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization |
| title_fullStr | Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization |
| title_full_unstemmed | Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization |
| title_short | Natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization |
| title_sort | natural nanotube-based biomimetic porous microspheres for significantly enhanced biomolecule immobilization |
| url | http://hdl.handle.net/20.500.11937/71716 |