A physiologically based kinetic model for elucidating the in vivo distribution of administered mesenchymal stem cells
Although mesenchymal stem cells (MSCs) present a promising tool in cell therapy for the treatment of various diseases, the in vivo distribution of administered MSCs has still been poorly understood, which hampers the precise prediction and evaluation of their therapeutic efficacy. Here, we developed...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Published: |
Nature Publishing Group
2016
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| Online Access: | http://hdl.handle.net/20.500.11937/71672 |
| _version_ | 1848762541562396672 |
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| author | Wang, H. Liang, X. Xu, Z. Crawford, D. Liu, Jian Roberts, M. |
| author_facet | Wang, H. Liang, X. Xu, Z. Crawford, D. Liu, Jian Roberts, M. |
| author_sort | Wang, H. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Although mesenchymal stem cells (MSCs) present a promising tool in cell therapy for the treatment of various diseases, the in vivo distribution of administered MSCs has still been poorly understood, which hampers the precise prediction and evaluation of their therapeutic efficacy. Here, we developed the first model to characterize the physiological kinetics of administered MSCs based on direct visualization of cell spatiotemporal disposition by intravital microscopy and assessment of cell quantity using flow cytometry. This physiologically based kinetic model was validated with multiple external datasets, indicating potential inter-route and inter-species predictive capability. Our results suggest that the targeting efficiency of MSCs is determined by the lung retention and interaction between MSCs and target organs, including cell arrest, depletion and release. By adapting specific parameters, this model can be easily applied to abnormal conditions or other types of circulating cells for designing treatment protocols and guiding future experiments. |
| first_indexed | 2025-11-14T10:49:12Z |
| format | Journal Article |
| id | curtin-20.500.11937-71672 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:49:12Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-716722019-01-22T07:06:01Z A physiologically based kinetic model for elucidating the in vivo distribution of administered mesenchymal stem cells Wang, H. Liang, X. Xu, Z. Crawford, D. Liu, Jian Roberts, M. Although mesenchymal stem cells (MSCs) present a promising tool in cell therapy for the treatment of various diseases, the in vivo distribution of administered MSCs has still been poorly understood, which hampers the precise prediction and evaluation of their therapeutic efficacy. Here, we developed the first model to characterize the physiological kinetics of administered MSCs based on direct visualization of cell spatiotemporal disposition by intravital microscopy and assessment of cell quantity using flow cytometry. This physiologically based kinetic model was validated with multiple external datasets, indicating potential inter-route and inter-species predictive capability. Our results suggest that the targeting efficiency of MSCs is determined by the lung retention and interaction between MSCs and target organs, including cell arrest, depletion and release. By adapting specific parameters, this model can be easily applied to abnormal conditions or other types of circulating cells for designing treatment protocols and guiding future experiments. 2016 Journal Article http://hdl.handle.net/20.500.11937/71672 10.1038/srep22293 http://creativecommons.org/licenses/by/4.0/ Nature Publishing Group fulltext |
| spellingShingle | Wang, H. Liang, X. Xu, Z. Crawford, D. Liu, Jian Roberts, M. A physiologically based kinetic model for elucidating the in vivo distribution of administered mesenchymal stem cells |
| title | A physiologically based kinetic model for elucidating the in vivo distribution of administered mesenchymal stem cells |
| title_full | A physiologically based kinetic model for elucidating the in vivo distribution of administered mesenchymal stem cells |
| title_fullStr | A physiologically based kinetic model for elucidating the in vivo distribution of administered mesenchymal stem cells |
| title_full_unstemmed | A physiologically based kinetic model for elucidating the in vivo distribution of administered mesenchymal stem cells |
| title_short | A physiologically based kinetic model for elucidating the in vivo distribution of administered mesenchymal stem cells |
| title_sort | physiologically based kinetic model for elucidating the in vivo distribution of administered mesenchymal stem cells |
| url | http://hdl.handle.net/20.500.11937/71672 |