Manipulation of the gut microbiota using resistant starch is associated with protection against colitis-associated colorectal cancer in rats
© The Author 2016. Published by Oxford University Press. All rights reserved. This study evaluated whether dietary resistant starch (RS) and green tea extract (GTE), which have anti-inflammatory and anticancer properties, protect against colitis-associated colorectal cancer (CAC) using a rat model,...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
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Oxford University Press
2015
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| Online Access: | http://hdl.handle.net/20.500.11937/71266 |
| _version_ | 1848762434436726784 |
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| author | Hu, Y. Le Leu, R. Christophersen, Claus Somashekar, R. Conlon, M. Meng, X. Winter, J. Woodman, R. McKinnon, R. Young, G. |
| author_facet | Hu, Y. Le Leu, R. Christophersen, Claus Somashekar, R. Conlon, M. Meng, X. Winter, J. Woodman, R. McKinnon, R. Young, G. |
| author_sort | Hu, Y. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © The Author 2016. Published by Oxford University Press. All rights reserved. This study evaluated whether dietary resistant starch (RS) and green tea extract (GTE), which have anti-inflammatory and anticancer properties, protect against colitis-associated colorectal cancer (CAC) using a rat model, also investigated potential mechanisms of action of these agents including their effects on the gut microbiota. Rats were fed a control diet or diets containing 10% RS, 0.5% GTE or a combination of the two (RS + GTE). CAC was initiated with 2 weekly azoxymethane (AOM) injections (10 mg/kg) followed by 2% dextran sodium sulphate in drinking water for 7 days after 2 weeks on diets. Rats were killed 20 weeks after the first AOM. Colon tissues and tumours were examined for histopathology by H&E, gene/protein expression by PCR and immunohistochemistry and digesta for analyses of fermentation products and microbiota populations. RS and RS + GTE (but not GTE) diets significantly (P < 0.05) decreased tumour multiplicity and adenocarcinoma formation, relative to the control diet. Effects of RS + GTE were not different from RS alone. RS diet caused significant shifts in microbial composition/diversity, with increases in Parabacteroides, Barnesiella, Ruminococcus, Marvinbryantia and Bifidobacterium as primary contributors to the shift. RS-containing diets increased short chain fatty acids (SCFA) and expression of the SCFA receptor GPR43 mRNA, and reduced inflammation (COX-2, NF-kB, TNF-a and IL-1ß mRNA) and cell proliferation P < 0.05. GTE had no effect. This is the first study that demonstrates chemopreventive effects of RS (but not GTE) in a rodent CAC model, suggesting RS might have benefit to patients with ulcerative colitis who are at an increased risk of developing CRC. |
| first_indexed | 2025-11-14T10:47:30Z |
| format | Journal Article |
| id | curtin-20.500.11937-71266 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:47:30Z |
| publishDate | 2015 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-712662018-12-13T09:34:20Z Manipulation of the gut microbiota using resistant starch is associated with protection against colitis-associated colorectal cancer in rats Hu, Y. Le Leu, R. Christophersen, Claus Somashekar, R. Conlon, M. Meng, X. Winter, J. Woodman, R. McKinnon, R. Young, G. © The Author 2016. Published by Oxford University Press. All rights reserved. This study evaluated whether dietary resistant starch (RS) and green tea extract (GTE), which have anti-inflammatory and anticancer properties, protect against colitis-associated colorectal cancer (CAC) using a rat model, also investigated potential mechanisms of action of these agents including their effects on the gut microbiota. Rats were fed a control diet or diets containing 10% RS, 0.5% GTE or a combination of the two (RS + GTE). CAC was initiated with 2 weekly azoxymethane (AOM) injections (10 mg/kg) followed by 2% dextran sodium sulphate in drinking water for 7 days after 2 weeks on diets. Rats were killed 20 weeks after the first AOM. Colon tissues and tumours were examined for histopathology by H&E, gene/protein expression by PCR and immunohistochemistry and digesta for analyses of fermentation products and microbiota populations. RS and RS + GTE (but not GTE) diets significantly (P < 0.05) decreased tumour multiplicity and adenocarcinoma formation, relative to the control diet. Effects of RS + GTE were not different from RS alone. RS diet caused significant shifts in microbial composition/diversity, with increases in Parabacteroides, Barnesiella, Ruminococcus, Marvinbryantia and Bifidobacterium as primary contributors to the shift. RS-containing diets increased short chain fatty acids (SCFA) and expression of the SCFA receptor GPR43 mRNA, and reduced inflammation (COX-2, NF-kB, TNF-a and IL-1ß mRNA) and cell proliferation P < 0.05. GTE had no effect. This is the first study that demonstrates chemopreventive effects of RS (but not GTE) in a rodent CAC model, suggesting RS might have benefit to patients with ulcerative colitis who are at an increased risk of developing CRC. 2015 Journal Article http://hdl.handle.net/20.500.11937/71266 10.1093/carcin/bgw019 Oxford University Press restricted |
| spellingShingle | Hu, Y. Le Leu, R. Christophersen, Claus Somashekar, R. Conlon, M. Meng, X. Winter, J. Woodman, R. McKinnon, R. Young, G. Manipulation of the gut microbiota using resistant starch is associated with protection against colitis-associated colorectal cancer in rats |
| title | Manipulation of the gut microbiota using resistant starch is associated with protection against colitis-associated colorectal cancer in rats |
| title_full | Manipulation of the gut microbiota using resistant starch is associated with protection against colitis-associated colorectal cancer in rats |
| title_fullStr | Manipulation of the gut microbiota using resistant starch is associated with protection against colitis-associated colorectal cancer in rats |
| title_full_unstemmed | Manipulation of the gut microbiota using resistant starch is associated with protection against colitis-associated colorectal cancer in rats |
| title_short | Manipulation of the gut microbiota using resistant starch is associated with protection against colitis-associated colorectal cancer in rats |
| title_sort | manipulation of the gut microbiota using resistant starch is associated with protection against colitis-associated colorectal cancer in rats |
| url | http://hdl.handle.net/20.500.11937/71266 |