Genetic variation of Pyrenophora teres f. teres isolates in Western Australia and emergence of a Cyp51A fungicide resistance mutation

Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. Genome-wide, unlinked, simple sequence repeat markers were used to examine genetic variation and relationships within Pyrenophora teres f. teres, a common pathogen of barley, in Western Austral...

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Main Authors: Ellwood, Simon, Piscetek, V., Mair, W., Lawrence, J., Lopez-Ruiz, Fran, Rawlinson, C.
Format: Journal Article
Published: Wiley-Blackwell 2018
Online Access:http://hdl.handle.net/20.500.11937/71103
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author Ellwood, Simon
Piscetek, V.
Mair, W.
Lawrence, J.
Lopez-Ruiz, Fran
Rawlinson, C.
author_facet Ellwood, Simon
Piscetek, V.
Mair, W.
Lawrence, J.
Lopez-Ruiz, Fran
Rawlinson, C.
author_sort Ellwood, Simon
building Curtin Institutional Repository
collection Online Access
description Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. Genome-wide, unlinked, simple sequence repeat markers were used to examine genetic variation and relationships within Pyrenophora teres f. teres, a common pathogen of barley, in Western Australia. Despite the region's geographic isolation, the isolates showed relatively high allelic variation compared to similar studies, averaging 7.11 alleles per locus. Principal component, Bayesian clustering and distance differentiation parameters provided evidence for both regional genotypic subdivision together with juxtaposing of isolates possessing different genetic backgrounds. Genotyping of fungicide resistant Cyp51A isolates indicated a single mutation event occurred followed by recombination and long-distance regional dispersal over hundreds of kilometres. Selection of recently emergent favourable alleles such as the Cyp51A mutation and a cultivar virulence may provide an explanation, at least in part, for juxtaposed genotypes. Factors affecting genotypic composition and the movement of new genotypes are discussed in the context of grower practices and pathogen epidemiology, together with the implications for resistance breeding.
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spelling curtin-20.500.11937-711032019-02-14T03:51:03Z Genetic variation of Pyrenophora teres f. teres isolates in Western Australia and emergence of a Cyp51A fungicide resistance mutation Ellwood, Simon Piscetek, V. Mair, W. Lawrence, J. Lopez-Ruiz, Fran Rawlinson, C. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology. Genome-wide, unlinked, simple sequence repeat markers were used to examine genetic variation and relationships within Pyrenophora teres f. teres, a common pathogen of barley, in Western Australia. Despite the region's geographic isolation, the isolates showed relatively high allelic variation compared to similar studies, averaging 7.11 alleles per locus. Principal component, Bayesian clustering and distance differentiation parameters provided evidence for both regional genotypic subdivision together with juxtaposing of isolates possessing different genetic backgrounds. Genotyping of fungicide resistant Cyp51A isolates indicated a single mutation event occurred followed by recombination and long-distance regional dispersal over hundreds of kilometres. Selection of recently emergent favourable alleles such as the Cyp51A mutation and a cultivar virulence may provide an explanation, at least in part, for juxtaposed genotypes. Factors affecting genotypic composition and the movement of new genotypes are discussed in the context of grower practices and pathogen epidemiology, together with the implications for resistance breeding. 2018 Journal Article http://hdl.handle.net/20.500.11937/71103 10.1111/ppa.12924 http://creativecommons.org/licenses/by-nc/4.0/ Wiley-Blackwell fulltext
spellingShingle Ellwood, Simon
Piscetek, V.
Mair, W.
Lawrence, J.
Lopez-Ruiz, Fran
Rawlinson, C.
Genetic variation of Pyrenophora teres f. teres isolates in Western Australia and emergence of a Cyp51A fungicide resistance mutation
title Genetic variation of Pyrenophora teres f. teres isolates in Western Australia and emergence of a Cyp51A fungicide resistance mutation
title_full Genetic variation of Pyrenophora teres f. teres isolates in Western Australia and emergence of a Cyp51A fungicide resistance mutation
title_fullStr Genetic variation of Pyrenophora teres f. teres isolates in Western Australia and emergence of a Cyp51A fungicide resistance mutation
title_full_unstemmed Genetic variation of Pyrenophora teres f. teres isolates in Western Australia and emergence of a Cyp51A fungicide resistance mutation
title_short Genetic variation of Pyrenophora teres f. teres isolates in Western Australia and emergence of a Cyp51A fungicide resistance mutation
title_sort genetic variation of pyrenophora teres f. teres isolates in western australia and emergence of a cyp51a fungicide resistance mutation
url http://hdl.handle.net/20.500.11937/71103