In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like Characteristics

QR Code

In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like Characteristics

The long-term expansion of keratinocytes under serum- and feeder free conditions generally results in diminished proliferation and an increased commitment to terminal differentiation. Here we present a serum and xenogeneic feeder free culture system that retains the self-renewal capacity of primary...

Full description

Bibliographic Details
Main Authors:	Wong, C., Kinnear, B., Sobota, R., Ramalingam, R., Legrand, C., Dye, Danielle, Raghunath, M., Lane, B., Coombe, D.
Format:	Journal Article
Published:	2018
Online Access:	http://hdl.handle.net/20.500.11937/71059

Similar Items

Dermal fibroblast extracellular matrix regulates keratinocyte behaviour
by: Wong, Chee Wai
Published: (2017)

Effect of dermal fibroblast conditioned medium on keratinocytes irrespective of age group
by: Nurul ‘Izzah Abdul Ghani,, et al.
Published: (2023)

Identification and Characterization of Intraoral and Dermal Fibroblasts Revisited
by: Mohd Nor, Nurul Hafizah, et al.
Published: (2017)

Interaction between skeletal muscle cells and extracellular matrix proteins using a serum free culture system
by: Dye, Danielle, et al.
Published: (2019)

The mRNA Expression Of Inducible Nitric Oxide Synthase In Keloid Using Tocotrienol-Rich Fraction In Primary Human Epidermal Keratinocytes And Primary Human Dermal Fibroblast Cultures
by: Yusuf, Siti Mahirah
Published: (2012)

Characterization of keratinocytes, fibroblasts and melanocytes isolated from human skin using gene markers
by: Wong, Pau-Ring, et al.
Published: (2022)

Silk fibroin scaffolds with muscle-like elasticity support in vitro differentiation of human skeletal muscle cells
by: Chaturvedi, V., et al.
Published: (2016)

Wound healing potential of Spirulina platensis extracts on human dermal fibroblast cells
by: Pauzi, Nur Aimi Syarina, et al.
Published: (2015)

In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
by: Abdull Rasad, Mohammad Syaiful Bahari, et al.
Published: (2010)

Exploring the potential of dermal fibroblast conditioned medium on skin wound healing and anti-ageing
by: Shiplu Roy Chowdhury,, et al.
Published: (2019)

Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast
by: Tithteeya Rattanachot,, et al.
Published: (2025)

Potential of stingless bee honey in modulating skin ageing of human dermal fibroblast cells
by: Malik, Nurfairuz Abdul
Published: (2021)

The basement membrane formation post implantation with bilayered fibrin-fibroblast/fibrin-keratinocytes skin equivalent during wound healing process.
by: Othman, Fauziah, et al.
Published: (2009)

Development of bovine cartilage extracellular matrix as a potential scaffold for chondrogenic induction of human dermal fibroblasts / Ali Moradi
by: Moradi, Ali
Published: (2015)

KGM hydrogels inhibit the contraction of tissue engineered skin and stimulate the proliferation of fibroblasts in the dermal area
by: Shahbuddin, Munira, et al.
Published: (2018)

Interactions between skeletal muscle myoblasts and their extracellular matrix revealed by a serum free culture system
by: Chaturvedi, Vishal, et al.
Published: (2015)

Human keratinocytes and fibroblast gene expressions were maintained even when trypsinized with recombinant trypsin: Towards clinical application
by: Khairul AK,, et al.
Published: (2011)

Keratinocyte growth factor 1, fibroblast growth factor 2 and 10 in the healing tympanic membrane following perforation in rats
by: Santa Maria, P., et al.
Published: (2011)

Maggots and wound healing: the effects of Lucilia sericata larval secretions upon interactions between human dermal fibroblasts and extracellular matrix proteins
by: Horobin, Adele Jayne
Published: (2005)

Expired platelet concentrate as a source of human platelet lysate for xenogeneic-free culture of human dermal fibroblasts
by: Muhammad Najib Fathi Hassan,, et al.
Published: (2021)

Cytotoxicity, proliferation and migration rate assessments of human dermal fibroblast adult cells using Zingiber zerumbet extract
by: Mazlyzam Abdul Latif,, et al.
Published: (2019)

Characterisation and cytotoxicity assessment of UV absorbers-intercalated zinc/aluminium-layered double hydroxides on dermal fibroblast cells
by: Megat Nabil Mohsin, Sumaiyah, et al.
Published: (2014)

Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts
by: Gothai, Sivapragasam, et al.
Published: (2017)

Feature, Structure and Classification of Adhesion Molecules: An Overview
by: Coombe, Deirdre, et al.
Published: (2010)

The Role of Immunoglobulin Superfamily Cell Adhesion Molecules in Cancer Metastasis
by: Wong, C., et al.
Published: (2012)

Biochemical evaluations of Zingiberaceae sp. and transcriptomics profiling of UV-irradiated normal human adult dermal fibroblast cells for anti-aging
by: Adekoya, Alafiatayo Akinola
Published: (2017)

Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts
by: Khaizurin Tajul Arifin,, et al.
Published: (2022)

Healing effect of Vicenin-2 (VCN-2) on Human Dermal Fibroblast (HDF) and development VCN-2 hydrocolloid film based on alginate as potential wound dressing
by: Tan, Woan Sean, et al.
Published: (2020)

Reflectance confocal microscopy for diagnosising keratinocyte skin cancers in adults
by: Dinnes, Jacqueline, et al.
Published: (2018)

Melanoma biomolecules: Independently identified but functionally intertwined
by: Dye, Danielle, et al.
Published: (2013)

Toxicity of zinc oxide nanoparticles on human skin dermal cells
by: Maheswaran, H., et al.
Published: (2021)

In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes
by: Gan, Yee-Lin, et al.
Published: (2024)

Basic fibroblast growth factor enhances the expansion and secretory profile of human placenta-derived mesenchymal stem cells
by: Vellasamy, Shalini, et al.
Published: (2016)

Antioxidative and photoprotective effects of pleurotus flabellatus (pink oyster mushroom) polysaccharides against UVA radiation-induced cytotoxicity in human dermal fibroblast (HS-27) cell line
by: Abdul Rahman, Norintan Suhaida, et al.
Published: (2021)

Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines
by: Santhanam, Ramesh Kumar, et al.
Published: (2016)

Bioactive scaffolds in skeletal muscle regeneration and tissue engineering
by: Chaturvedi, Vishal, et al.
Published: (2011)

Extracellular matrix glycoproteins of skin fibroblasts in tuberous sclerosis
by: Uysal, Hamdi
Published: (1995)

Visual examination and dermoscopy, alone or in combination, for the diagnosis of keratinocyte skin cancers in adults
by: Dinnes, Jacqueline, et al.
Published: (2018)

Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue Regeneration
by: Mohammadzadehmoghadam, Soheila, et al.
Published: (2022)

ECM crosslinking enhances fibroblast growth and protects against matrix proteolysis in lung fibrosis
by: Philp, Christopher J., et al.
Published: (2017)