Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity,...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/70884 |
| _version_ | 1848762330820640768 |
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| author | Wang, H. Liang, X. Gravot, G. Thorling, C. Crawford, D. Xu, Z. Liu, Jian Roberts, M. |
| author_facet | Wang, H. Liang, X. Gravot, G. Thorling, C. Crawford, D. Xu, Z. Liu, Jian Roberts, M. |
| author_sort | Wang, H. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases. (Figure presented.). |
| first_indexed | 2025-11-14T10:45:51Z |
| format | Journal Article |
| id | curtin-20.500.11937-70884 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:45:51Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-708842018-12-13T09:34:31Z Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy Wang, H. Liang, X. Gravot, G. Thorling, C. Crawford, D. Xu, Z. Liu, Jian Roberts, M. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases. (Figure presented.). 2017 Journal Article http://hdl.handle.net/20.500.11937/70884 10.1002/jbio.201600083 restricted |
| spellingShingle | Wang, H. Liang, X. Gravot, G. Thorling, C. Crawford, D. Xu, Z. Liu, Jian Roberts, M. Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy |
| title | Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy |
| title_full | Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy |
| title_fullStr | Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy |
| title_full_unstemmed | Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy |
| title_short | Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy |
| title_sort | visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy |
| url | http://hdl.handle.net/20.500.11937/70884 |