Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria
There is growing evidence for bacteria playing a role in the pathogenesis and formation of pigmented gallstones from humans. These studies mainly involved cultivation of gallstone-associated bacteria and 16S rRNA profiling, providing an indirect link between processes involved in gallstone formation...
| Main Authors: | , , , , |
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| Format: | Journal Article |
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Nature Publishing Group
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/70253 |
| _version_ | 1848762255987965952 |
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| author | Kose, Sureyya Grice, Kliti Orsi, W. Ballal, M. Coolen, Marco |
| author_facet | Kose, Sureyya Grice, Kliti Orsi, W. Ballal, M. Coolen, Marco |
| author_sort | Kose, Sureyya |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | There is growing evidence for bacteria playing a role in the pathogenesis and formation of pigmented gallstones from humans. These studies mainly involved cultivation of gallstone-associated bacteria and 16S rRNA profiling, providing an indirect link between processes involved in gallstone formation by the bacteria in-situ. Here, we provide functional metagenomic evidence of a range of genes involved in bile stress response, biofilm formation, and anaerobic energy metabolism by Gram-negative Klebsiella in pigmented gallstones from a 76-year-old male patient. Klebsiella was also present in one cholesterol-type stone in a 30-year-old female patient who had additional cholesterol gallstones characterised by Gram-positive bacteria. Pigmented stones further revealed a predominance of genes involved in carbohydrate metabolism, whilst cholesterol stones indicated a profile dominanted by protein metabolism possibly reflecting known chemical differences between Gram-negative and Gram-positive biofilm matrices. Archaeal genes were not detected. Complementary carbon and hydrogen isotopic analyses of cholesterol within the patients' stones revealed homogeneity, suggesting a common diet or cholesterol biosynthesis pathway that has little influence on microbial composition. This pilot study provides a framework to study microbial processes that play a potential role in gallstone formation across markedly different types of stones and patient backgrounds. |
| first_indexed | 2025-11-14T10:44:40Z |
| format | Journal Article |
| id | curtin-20.500.11937-70253 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:44:40Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-702532018-09-17T04:59:38Z Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria Kose, Sureyya Grice, Kliti Orsi, W. Ballal, M. Coolen, Marco There is growing evidence for bacteria playing a role in the pathogenesis and formation of pigmented gallstones from humans. These studies mainly involved cultivation of gallstone-associated bacteria and 16S rRNA profiling, providing an indirect link between processes involved in gallstone formation by the bacteria in-situ. Here, we provide functional metagenomic evidence of a range of genes involved in bile stress response, biofilm formation, and anaerobic energy metabolism by Gram-negative Klebsiella in pigmented gallstones from a 76-year-old male patient. Klebsiella was also present in one cholesterol-type stone in a 30-year-old female patient who had additional cholesterol gallstones characterised by Gram-positive bacteria. Pigmented stones further revealed a predominance of genes involved in carbohydrate metabolism, whilst cholesterol stones indicated a profile dominanted by protein metabolism possibly reflecting known chemical differences between Gram-negative and Gram-positive biofilm matrices. Archaeal genes were not detected. Complementary carbon and hydrogen isotopic analyses of cholesterol within the patients' stones revealed homogeneity, suggesting a common diet or cholesterol biosynthesis pathway that has little influence on microbial composition. This pilot study provides a framework to study microbial processes that play a potential role in gallstone formation across markedly different types of stones and patient backgrounds. 2018 Journal Article http://hdl.handle.net/20.500.11937/70253 10.1038/s41598-018-29571-8 http://creativecommons.org/licenses/by/4.0/ Nature Publishing Group fulltext |
| spellingShingle | Kose, Sureyya Grice, Kliti Orsi, W. Ballal, M. Coolen, Marco Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria |
| title | Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria |
| title_full | Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria |
| title_fullStr | Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria |
| title_full_unstemmed | Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria |
| title_short | Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria |
| title_sort | metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria |
| url | http://hdl.handle.net/20.500.11937/70253 |