An ultrasensitive electrochemical sensing platform for Hg2+ based on a density controllable metal-organic hybrid microarray
A novel electrochemical Hg2+biosensor was developed on the basis of a metal-organic hybrid microarray, in which the nicking endonuclease (NE) assisted target-triggered strand release strategy was realized via the DNA cyclic amplification technique. The metal-organic hybrid microarray was fabricated...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Published: |
Elsevier BV
2014
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| Online Access: | http://hdl.handle.net/20.500.11937/70213 |
| _version_ | 1848762245248450560 |
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| author | Shi, L. Chu, Z. Liu, Yu Jin, W. Chen, X. |
| author_facet | Shi, L. Chu, Z. Liu, Yu Jin, W. Chen, X. |
| author_sort | Shi, L. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | A novel electrochemical Hg2+biosensor was developed on the basis of a metal-organic hybrid microarray, in which the nicking endonuclease (NE) assisted target-triggered strand release strategy was realized via the DNA cyclic amplification technique. The metal-organic hybrid microarray was fabricated using the SAM of 1, 4-benzenendithiol as soft template, and the density of the microarray could be adjusted by controlling the surface coverage of 1,4-benzenendithiol molecules. In the presence of Hg2+, capture DNA (cDNA) with an indicator at one end could hybridize with the reporter DNA (rDNA) through the stable T-Hg2+-T linkage, forming the nicking recognition site. After the nicking reaction, the electrochemical indicator dissociated from the electrode surface. The released rDNA and Hg2+could be reused in the sensing system and initiate the next cycle, and more electroactive indicator dissociated from the electrode surface, resulting in a significant signal decrease. The constructed DNA biosensor could detect Hg2+in a wide linear range from 15pM to 500nM, with an ultrasensitive detection limit of 5pM (S/N=3). Furthermore, the biosensor exhibited excellent stability, good reproducibility and high selectivity towards other divalent ions. The proposed sensing system also showed a promising potential for the application in real aquatic product sample analysis.© 2013 Elsevier B.V. |
| first_indexed | 2025-11-14T10:44:30Z |
| format | Journal Article |
| id | curtin-20.500.11937-70213 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:44:30Z |
| publishDate | 2014 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-702132018-11-14T07:39:16Z An ultrasensitive electrochemical sensing platform for Hg2+ based on a density controllable metal-organic hybrid microarray Shi, L. Chu, Z. Liu, Yu Jin, W. Chen, X. A novel electrochemical Hg2+biosensor was developed on the basis of a metal-organic hybrid microarray, in which the nicking endonuclease (NE) assisted target-triggered strand release strategy was realized via the DNA cyclic amplification technique. The metal-organic hybrid microarray was fabricated using the SAM of 1, 4-benzenendithiol as soft template, and the density of the microarray could be adjusted by controlling the surface coverage of 1,4-benzenendithiol molecules. In the presence of Hg2+, capture DNA (cDNA) with an indicator at one end could hybridize with the reporter DNA (rDNA) through the stable T-Hg2+-T linkage, forming the nicking recognition site. After the nicking reaction, the electrochemical indicator dissociated from the electrode surface. The released rDNA and Hg2+could be reused in the sensing system and initiate the next cycle, and more electroactive indicator dissociated from the electrode surface, resulting in a significant signal decrease. The constructed DNA biosensor could detect Hg2+in a wide linear range from 15pM to 500nM, with an ultrasensitive detection limit of 5pM (S/N=3). Furthermore, the biosensor exhibited excellent stability, good reproducibility and high selectivity towards other divalent ions. The proposed sensing system also showed a promising potential for the application in real aquatic product sample analysis.© 2013 Elsevier B.V. 2014 Journal Article http://hdl.handle.net/20.500.11937/70213 10.1016/j.bios.2013.10.074 Elsevier BV restricted |
| spellingShingle | Shi, L. Chu, Z. Liu, Yu Jin, W. Chen, X. An ultrasensitive electrochemical sensing platform for Hg2+ based on a density controllable metal-organic hybrid microarray |
| title | An ultrasensitive electrochemical sensing platform for Hg2+ based on a density controllable metal-organic hybrid microarray |
| title_full | An ultrasensitive electrochemical sensing platform for Hg2+ based on a density controllable metal-organic hybrid microarray |
| title_fullStr | An ultrasensitive electrochemical sensing platform for Hg2+ based on a density controllable metal-organic hybrid microarray |
| title_full_unstemmed | An ultrasensitive electrochemical sensing platform for Hg2+ based on a density controllable metal-organic hybrid microarray |
| title_short | An ultrasensitive electrochemical sensing platform for Hg2+ based on a density controllable metal-organic hybrid microarray |
| title_sort | ultrasensitive electrochemical sensing platform for hg2+ based on a density controllable metal-organic hybrid microarray |
| url | http://hdl.handle.net/20.500.11937/70213 |