Origin, evolution, and global transmission of community-acquired Staphylococcus aureus ST8
USA300 is a pandemic clonal lineage of hypervirulent, community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) with specific molecular characteristics. Despite its high clinical relevance, the evolutionary origin of USA300 remained unclear. We used comparative genomics of 224 tempor...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
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National Academy of Sciences
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/70109 |
| _version_ | 1848762217765273600 |
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| author | Strauß, L. Stegger, M. Akpaka, P. Alabi, A. Breurec, S. Coombs, Geoffrey Egyir, B. Larsen, A. Laurent, F. Monecke, S. Peters, G. Skov, R. Strommenger, B. Vandenesch, F. Schaumburg, F. Mellmann, A. |
| author_facet | Strauß, L. Stegger, M. Akpaka, P. Alabi, A. Breurec, S. Coombs, Geoffrey Egyir, B. Larsen, A. Laurent, F. Monecke, S. Peters, G. Skov, R. Strommenger, B. Vandenesch, F. Schaumburg, F. Mellmann, A. |
| author_sort | Strauß, L. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | USA300 is a pandemic clonal lineage of hypervirulent, community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) with specific molecular characteristics. Despite its high clinical relevance, the evolutionary origin of USA300 remained unclear. We used comparative genomics of 224 temporal and spatial diverse S. aureus isolates of multilocus sequence type (ST) 8 to reconstruct the molecular evolution and global dissemination of ST8, including USA300. Analyses of core SNP diversity and accessory genome variations showed that the ancestor of all ST8 S. aureus most likely emerged in Central Europe in the mid-19th century. From here, ST8 was exported to North America in the early 20th century and progressively acquired the USA300 characteristics Panton–Valentine leukocidin (PVL), SCCmec IVa, the arginine catabolic mobile element (ACME), and a specific mutation in capsular polysaccharide gene cap5E. Although the PVL-encoding phage ?Sa2USA was introduced into the ST8 background only once, various SCCmec types were introduced to ST8 at different times and places. Starting from North America, USA300 spread globally, including Africa. African USA300 isolates have aberrant spa-types (t112, t121) and form a monophyletic group within the clade of North American USA300. Large parts of ST8 methicillin-susceptible S. aureus (MSSA) isolated in Africa represent a symplesiomorphic group of ST8 (i.e., a group representing the characteristics of the ancestor), which are rarely found in other world regions. Isolates previously discussed as USA300 ancestors, including USA500 and a “historic” CA-MRSA from Western Australia, were shown to be only distantly related to recent USA300 clones. |
| first_indexed | 2025-11-14T10:44:04Z |
| format | Journal Article |
| id | curtin-20.500.11937-70109 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:44:04Z |
| publishDate | 2017 |
| publisher | National Academy of Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-701092018-09-27T06:31:56Z Origin, evolution, and global transmission of community-acquired Staphylococcus aureus ST8 Strauß, L. Stegger, M. Akpaka, P. Alabi, A. Breurec, S. Coombs, Geoffrey Egyir, B. Larsen, A. Laurent, F. Monecke, S. Peters, G. Skov, R. Strommenger, B. Vandenesch, F. Schaumburg, F. Mellmann, A. USA300 is a pandemic clonal lineage of hypervirulent, community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) with specific molecular characteristics. Despite its high clinical relevance, the evolutionary origin of USA300 remained unclear. We used comparative genomics of 224 temporal and spatial diverse S. aureus isolates of multilocus sequence type (ST) 8 to reconstruct the molecular evolution and global dissemination of ST8, including USA300. Analyses of core SNP diversity and accessory genome variations showed that the ancestor of all ST8 S. aureus most likely emerged in Central Europe in the mid-19th century. From here, ST8 was exported to North America in the early 20th century and progressively acquired the USA300 characteristics Panton–Valentine leukocidin (PVL), SCCmec IVa, the arginine catabolic mobile element (ACME), and a specific mutation in capsular polysaccharide gene cap5E. Although the PVL-encoding phage ?Sa2USA was introduced into the ST8 background only once, various SCCmec types were introduced to ST8 at different times and places. Starting from North America, USA300 spread globally, including Africa. African USA300 isolates have aberrant spa-types (t112, t121) and form a monophyletic group within the clade of North American USA300. Large parts of ST8 methicillin-susceptible S. aureus (MSSA) isolated in Africa represent a symplesiomorphic group of ST8 (i.e., a group representing the characteristics of the ancestor), which are rarely found in other world regions. Isolates previously discussed as USA300 ancestors, including USA500 and a “historic” CA-MRSA from Western Australia, were shown to be only distantly related to recent USA300 clones. 2017 Journal Article http://hdl.handle.net/20.500.11937/70109 10.1073/pnas.1702472114 National Academy of Sciences restricted |
| spellingShingle | Strauß, L. Stegger, M. Akpaka, P. Alabi, A. Breurec, S. Coombs, Geoffrey Egyir, B. Larsen, A. Laurent, F. Monecke, S. Peters, G. Skov, R. Strommenger, B. Vandenesch, F. Schaumburg, F. Mellmann, A. Origin, evolution, and global transmission of community-acquired Staphylococcus aureus ST8 |
| title | Origin, evolution, and global transmission of community-acquired Staphylococcus aureus ST8 |
| title_full | Origin, evolution, and global transmission of community-acquired Staphylococcus aureus ST8 |
| title_fullStr | Origin, evolution, and global transmission of community-acquired Staphylococcus aureus ST8 |
| title_full_unstemmed | Origin, evolution, and global transmission of community-acquired Staphylococcus aureus ST8 |
| title_short | Origin, evolution, and global transmission of community-acquired Staphylococcus aureus ST8 |
| title_sort | origin, evolution, and global transmission of community-acquired staphylococcus aureus st8 |
| url | http://hdl.handle.net/20.500.11937/70109 |