Method development for quantification of bromochloramine using membrane introduction mass spectrometry

During chloramination of bromide-containing water, the main brominated-amine formed is bromochloramine (NHBrCl). To date, there is no analytical method, free of interference, allowing its accurate quantification. The major reason is that it is not possible to produce a pure NHBrCl solution. In this...

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Main Authors: Allard, Sebastien, Hu, W., Le Menn, J., Cadee, Keith, Gallard, H., Croue, Jean-Philippe
Format: Journal Article
Published: American Chemical Society 2018
Online Access:http://hdl.handle.net/20.500.11937/69925
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author Allard, Sebastien
Hu, W.
Le Menn, J.
Cadee, Keith
Gallard, H.
Croue, Jean-Philippe
author_facet Allard, Sebastien
Hu, W.
Le Menn, J.
Cadee, Keith
Gallard, H.
Croue, Jean-Philippe
author_sort Allard, Sebastien
building Curtin Institutional Repository
collection Online Access
description During chloramination of bromide-containing water, the main brominated-amine formed is bromochloramine (NHBrCl). To date, there is no analytical method, free of interference, allowing its accurate quantification. The major reason is that it is not possible to produce a pure NHBrCl solution. In this study, we report a method allowing the accurate quantification of NHBrCl with membrane introduction mass spectrometry (MIMS). Firstly, the molar absorption coefficient for NHBrCl was determined by quantifying NHBrCl as 2,4,6-tribromophenol by HPLC-UV and comparing the results with the direct UV response at 320 nm. A molar absorption coefficient of 304 M-1cm-1 was obtained. The results obtained by direct UV measurements were compared to the MIMS signal recorded at m/z 131 corresponding to the mass of the molecular ion, and used to establish a calibration curve. A limit of detection of 2.9 µM was determined. MIMS is the only method enabling the unambiguous quantification of NHBrCl, as it is based on m/z 131, while with other analytical techniques, other halamines can interfere, i.e. overlapping peaks with direct UV measurements, reaction of several halamines with colorimetric reagents or phenols. This analytical method will benefit to the scientific community by allowing for example, further mechanistic study on the contribution of NHBrCl to the formation of toxic disinfection by products.
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spelling curtin-20.500.11937-699252018-10-16T03:32:43Z Method development for quantification of bromochloramine using membrane introduction mass spectrometry Allard, Sebastien Hu, W. Le Menn, J. Cadee, Keith Gallard, H. Croue, Jean-Philippe During chloramination of bromide-containing water, the main brominated-amine formed is bromochloramine (NHBrCl). To date, there is no analytical method, free of interference, allowing its accurate quantification. The major reason is that it is not possible to produce a pure NHBrCl solution. In this study, we report a method allowing the accurate quantification of NHBrCl with membrane introduction mass spectrometry (MIMS). Firstly, the molar absorption coefficient for NHBrCl was determined by quantifying NHBrCl as 2,4,6-tribromophenol by HPLC-UV and comparing the results with the direct UV response at 320 nm. A molar absorption coefficient of 304 M-1cm-1 was obtained. The results obtained by direct UV measurements were compared to the MIMS signal recorded at m/z 131 corresponding to the mass of the molecular ion, and used to establish a calibration curve. A limit of detection of 2.9 µM was determined. MIMS is the only method enabling the unambiguous quantification of NHBrCl, as it is based on m/z 131, while with other analytical techniques, other halamines can interfere, i.e. overlapping peaks with direct UV measurements, reaction of several halamines with colorimetric reagents or phenols. This analytical method will benefit to the scientific community by allowing for example, further mechanistic study on the contribution of NHBrCl to the formation of toxic disinfection by products. 2018 Journal Article http://hdl.handle.net/20.500.11937/69925 10.1021/acs.est.8b00889 American Chemical Society restricted
spellingShingle Allard, Sebastien
Hu, W.
Le Menn, J.
Cadee, Keith
Gallard, H.
Croue, Jean-Philippe
Method development for quantification of bromochloramine using membrane introduction mass spectrometry
title Method development for quantification of bromochloramine using membrane introduction mass spectrometry
title_full Method development for quantification of bromochloramine using membrane introduction mass spectrometry
title_fullStr Method development for quantification of bromochloramine using membrane introduction mass spectrometry
title_full_unstemmed Method development for quantification of bromochloramine using membrane introduction mass spectrometry
title_short Method development for quantification of bromochloramine using membrane introduction mass spectrometry
title_sort method development for quantification of bromochloramine using membrane introduction mass spectrometry
url http://hdl.handle.net/20.500.11937/69925