Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis
Methicillin-resistant coagulase-negative staphylococci (MRCoNS) have recently emerged as a significant cause of bovine mastitis worldwide. Here we describe the isolation of MRCoNS from cases of bovine mastitis from a single dairy farm in Australia. Fourteen CoNS isolates were identified as MRCoNS on...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
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Elsevier
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/69843 |
| _version_ | 1848762148433428480 |
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| author | Khazandi, M. Al-Farha, A. Coombs, Geoffrey O'Dea, M. Pang, S. Trott, D. Aviles, R. Hemmatzadeh, F. Venter, H. Ogunniyi, A. Hoare, A. Abraham, S. Petrovski, K. |
| author_facet | Khazandi, M. Al-Farha, A. Coombs, Geoffrey O'Dea, M. Pang, S. Trott, D. Aviles, R. Hemmatzadeh, F. Venter, H. Ogunniyi, A. Hoare, A. Abraham, S. Petrovski, K. |
| author_sort | Khazandi, M. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Methicillin-resistant coagulase-negative staphylococci (MRCoNS) have recently emerged as a significant cause of bovine mastitis worldwide. Here we describe the isolation of MRCoNS from cases of bovine mastitis from a single dairy farm in Australia. Fourteen CoNS isolates were identified as MRCoNS on the basis of having an oxacillin MIC of =0.5 µg/mL. The isolates were speciated as S. chromogenes (n = 1) S. fleurettii (n = 1), S. haemolyticus (n = 2), S. sciuri (n = 5), S. simulans (n = 1) S. succinus (n = 2) and S. xylosus (n = 2). Five of the isolates (S. fleuretti, S. haemolyticus S. sciuri and two S. succinus) were mecA-positive. We also detected a previously described S. sciuri mecA homolog in four oxacillin-resistant S. sciuri isolates. The remainder of the putative MRCoNS did not contain any mecA-related resistance determinants in their genomes. Comparative genomic analysis of three previously published S. sciuri isolates, from humans, a squirrel and a cereal crop (rice), and a representative isolate from our study demonstrated clustering and a high degree of genetic homogeneity (>95%), suggesting S. sciuri has low host specificity. In conclusion, CoNS, in particular S. sciuri, may act as a reservoir for SCCmec elements that can easily be spread between different host species by direct cross-infection. |
| first_indexed | 2025-11-14T10:42:58Z |
| format | Journal Article |
| id | curtin-20.500.11937-69843 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:42:58Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-698432018-09-27T06:26:13Z Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis Khazandi, M. Al-Farha, A. Coombs, Geoffrey O'Dea, M. Pang, S. Trott, D. Aviles, R. Hemmatzadeh, F. Venter, H. Ogunniyi, A. Hoare, A. Abraham, S. Petrovski, K. Methicillin-resistant coagulase-negative staphylococci (MRCoNS) have recently emerged as a significant cause of bovine mastitis worldwide. Here we describe the isolation of MRCoNS from cases of bovine mastitis from a single dairy farm in Australia. Fourteen CoNS isolates were identified as MRCoNS on the basis of having an oxacillin MIC of =0.5 µg/mL. The isolates were speciated as S. chromogenes (n = 1) S. fleurettii (n = 1), S. haemolyticus (n = 2), S. sciuri (n = 5), S. simulans (n = 1) S. succinus (n = 2) and S. xylosus (n = 2). Five of the isolates (S. fleuretti, S. haemolyticus S. sciuri and two S. succinus) were mecA-positive. We also detected a previously described S. sciuri mecA homolog in four oxacillin-resistant S. sciuri isolates. The remainder of the putative MRCoNS did not contain any mecA-related resistance determinants in their genomes. Comparative genomic analysis of three previously published S. sciuri isolates, from humans, a squirrel and a cereal crop (rice), and a representative isolate from our study demonstrated clustering and a high degree of genetic homogeneity (>95%), suggesting S. sciuri has low host specificity. In conclusion, CoNS, in particular S. sciuri, may act as a reservoir for SCCmec elements that can easily be spread between different host species by direct cross-infection. 2018 Journal Article http://hdl.handle.net/20.500.11937/69843 10.1016/j.vetmic.2018.04.004 Elsevier restricted |
| spellingShingle | Khazandi, M. Al-Farha, A. Coombs, Geoffrey O'Dea, M. Pang, S. Trott, D. Aviles, R. Hemmatzadeh, F. Venter, H. Ogunniyi, A. Hoare, A. Abraham, S. Petrovski, K. Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis |
| title | Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis |
| title_full | Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis |
| title_fullStr | Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis |
| title_full_unstemmed | Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis |
| title_short | Genomic characterization of coagulase-negative staphylococci including methicillin-resistant Staphylococcus sciuri causing bovine mastitis |
| title_sort | genomic characterization of coagulase-negative staphylococci including methicillin-resistant staphylococcus sciuri causing bovine mastitis |
| url | http://hdl.handle.net/20.500.11937/69843 |