Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode
As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
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Humana Press
2018
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| Online Access: | http://hdl.handle.net/20.500.11937/69785 |
| _version_ | 1848762133926379520 |
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| author | Buzid, A. Luong, J. Reen, F. O'Gara, Fergal Glennon, J. McGlacken, G. |
| author_facet | Buzid, A. Luong, J. Reen, F. O'Gara, Fergal Glennon, J. McGlacken, G. |
| author_sort | Buzid, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8 h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane. |
| first_indexed | 2025-11-14T10:42:44Z |
| format | Journal Article |
| id | curtin-20.500.11937-69785 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:42:44Z |
| publishDate | 2018 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-697852018-09-27T05:31:03Z Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode Buzid, A. Luong, J. Reen, F. O'Gara, Fergal Glennon, J. McGlacken, G. As the leading cause of morbidity and mortality of cystic fibrosis (CF) patients, early detection of Pseudomonas aeruginosa (PA) is critical in the clinical management of this pathogen. Herein, we describe rapid and sensitive electroanalytical methods using differential pulse voltammetry (DPV) at a boron-doped diamond (BDD) electrode for the detection of PA signaling biomolecules. Monitoring the production of key signaling molecules in bacterial cultures of P. aeruginosa PA14 over 8 h is described, involving sample pretreatment by liquid-liquid and solid-phase extraction. In addition, direct electrochemical detection approach of PA signaling molecules is also reported in conjunction with hexadecyltrimethylammonium bromide (CTAB) to disrupt the bacterial membrane. 2018 Journal Article http://hdl.handle.net/20.500.11937/69785 10.1007/978-1-4939-7309-5_9 Humana Press restricted |
| spellingShingle | Buzid, A. Luong, J. Reen, F. O'Gara, Fergal Glennon, J. McGlacken, G. Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode |
| title | Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode |
| title_full | Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode |
| title_fullStr | Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode |
| title_full_unstemmed | Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode |
| title_short | Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode |
| title_sort | rapid electrochemical detection of pseudomonas aeruginosa signaling molecules by boron-doped diamond electrode |
| url | http://hdl.handle.net/20.500.11937/69785 |