DOPE–oleic acid–Ca2+ as DNA condensing agent
Phospholipid-based non-viral carriers composed of neutral phospholipid dioleoylphosphatidylethanolamine (DOPE) and the binary mixture DOPE-oleic acid (OA) are examined as potential DNA delivery vectors. The process of DNA condensation in the presence of Ca2+ions has been monitored through changes in...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal Article |
| Published: |
2018
|
| Online Access: | http://hdl.handle.net/20.500.11937/69628 |
| _version_ | 1848762091732729856 |
|---|---|
| author | Galliková, D. Liskayová, G. Búcsi, A. Hubcík, L. Martinez, Jorge Uhríková, D. |
| author_facet | Galliková, D. Liskayová, G. Búcsi, A. Hubcík, L. Martinez, Jorge Uhríková, D. |
| author_sort | Galliková, D. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Phospholipid-based non-viral carriers composed of neutral phospholipid dioleoylphosphatidylethanolamine (DOPE) and the binary mixture DOPE-oleic acid (OA) are examined as potential DNA delivery vectors. The process of DNA condensation in the presence of Ca2+ions has been monitored through changes in emmision intensity of fluorescent probe ethidium bromide. The decline in fluorescence intensity with increasing Ca2+concentration at two different time intervals was correlated with the binding capacity of complexes and possible release of DNA from the complex. The microstructure of DOPE-OA mixtures at different OA/DOPE molar ratios and that of DOPE-OA-DNA-Ca2+complexes were determined using synchrotron small angle X-ray diffraction (SAXD). We identified inverted hexagonal phase HIIas the dominant structure. OA affects the lattice parameter of HIIformed by DOPE. With the increasing OA/DOPE molar ratio, the lattice parameter decreases, which results in significantly lower fraction of DNA bound to the OA-enriched complexes. |
| first_indexed | 2025-11-14T10:42:03Z |
| format | Journal Article |
| id | curtin-20.500.11937-69628 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:42:03Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-696282018-09-25T02:58:28Z DOPE–oleic acid–Ca2+ as DNA condensing agent Galliková, D. Liskayová, G. Búcsi, A. Hubcík, L. Martinez, Jorge Uhríková, D. Phospholipid-based non-viral carriers composed of neutral phospholipid dioleoylphosphatidylethanolamine (DOPE) and the binary mixture DOPE-oleic acid (OA) are examined as potential DNA delivery vectors. The process of DNA condensation in the presence of Ca2+ions has been monitored through changes in emmision intensity of fluorescent probe ethidium bromide. The decline in fluorescence intensity with increasing Ca2+concentration at two different time intervals was correlated with the binding capacity of complexes and possible release of DNA from the complex. The microstructure of DOPE-OA mixtures at different OA/DOPE molar ratios and that of DOPE-OA-DNA-Ca2+complexes were determined using synchrotron small angle X-ray diffraction (SAXD). We identified inverted hexagonal phase HIIas the dominant structure. OA affects the lattice parameter of HIIformed by DOPE. With the increasing OA/DOPE molar ratio, the lattice parameter decreases, which results in significantly lower fraction of DNA bound to the OA-enriched complexes. 2018 Journal Article http://hdl.handle.net/20.500.11937/69628 10.2478/afpuc-2018-0001 http://creativecommons.org/licenses/by-nc-nd/3.0/ fulltext |
| spellingShingle | Galliková, D. Liskayová, G. Búcsi, A. Hubcík, L. Martinez, Jorge Uhríková, D. DOPE–oleic acid–Ca2+ as DNA condensing agent |
| title | DOPE–oleic acid–Ca2+ as DNA condensing agent |
| title_full | DOPE–oleic acid–Ca2+ as DNA condensing agent |
| title_fullStr | DOPE–oleic acid–Ca2+ as DNA condensing agent |
| title_full_unstemmed | DOPE–oleic acid–Ca2+ as DNA condensing agent |
| title_short | DOPE–oleic acid–Ca2+ as DNA condensing agent |
| title_sort | dope–oleic acid–ca2+ as dna condensing agent |
| url | http://hdl.handle.net/20.500.11937/69628 |