Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex
© 2018 The Author(s). Mitochondrial morphology is important for the function of this critical organelle and, accordingly, altered mitochondrial structure is exhibited in many pathologies. Imaging of mitochondria can therefore provide important information about disease presence and progression. Howe...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
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Nature Publishing Group
2018
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| Online Access: | http://purl.org/au-research/grants/arc/FT130100033 http://hdl.handle.net/20.500.11937/69167 |
| _version_ | 1848761985633615872 |
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| author | Sorvina, A. Bader, C. Darby, J. Lock, M. Soo, J. Johnson, I. Caporale, Chiara Voelcker, N. Stagni, S. Massi, Massimiliano Morrison, J. Plush, S. Brooks, D. |
| author_facet | Sorvina, A. Bader, C. Darby, J. Lock, M. Soo, J. Johnson, I. Caporale, Chiara Voelcker, N. Stagni, S. Massi, Massimiliano Morrison, J. Plush, S. Brooks, D. |
| author_sort | Sorvina, A. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2018 The Author(s). Mitochondrial morphology is important for the function of this critical organelle and, accordingly, altered mitochondrial structure is exhibited in many pathologies. Imaging of mitochondria can therefore provide important information about disease presence and progression. However, mitochondrial imaging is currently limited by the availability of agents that have the capacity to image mitochondrial morphology in both live and fixed samples. This can be particularly problematic in clinical studies or large, multi-centre cohort studies, where tissue archiving by fixation is often more practical. We previously reported the synthesis of an iridium coordination complex [Ir(ppy)2(MeTzPyPhCN)]+; where ppy is a cyclometalated 2-phenylpyridine and TzPyPhCN is the 5-(5-(4-cyanophen-1-yl)pyrid-2-yl)tetrazolate ligand; and showed that this complex (herein referred to as IraZolve-Mito) has a high specificity for mitochondria in live cells. Here we demonstrate that IraZolve-Mito can also effectively stain mitochondria in both live and fixed tissue samples. The staining protocol proposed is versatile, providing a universal procedure for cell biologists and pathologists to visualise mitochondria. |
| first_indexed | 2025-11-14T10:40:22Z |
| format | Journal Article |
| id | curtin-20.500.11937-69167 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:40:22Z |
| publishDate | 2018 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-691672022-11-23T05:57:34Z Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex Sorvina, A. Bader, C. Darby, J. Lock, M. Soo, J. Johnson, I. Caporale, Chiara Voelcker, N. Stagni, S. Massi, Massimiliano Morrison, J. Plush, S. Brooks, D. © 2018 The Author(s). Mitochondrial morphology is important for the function of this critical organelle and, accordingly, altered mitochondrial structure is exhibited in many pathologies. Imaging of mitochondria can therefore provide important information about disease presence and progression. However, mitochondrial imaging is currently limited by the availability of agents that have the capacity to image mitochondrial morphology in both live and fixed samples. This can be particularly problematic in clinical studies or large, multi-centre cohort studies, where tissue archiving by fixation is often more practical. We previously reported the synthesis of an iridium coordination complex [Ir(ppy)2(MeTzPyPhCN)]+; where ppy is a cyclometalated 2-phenylpyridine and TzPyPhCN is the 5-(5-(4-cyanophen-1-yl)pyrid-2-yl)tetrazolate ligand; and showed that this complex (herein referred to as IraZolve-Mito) has a high specificity for mitochondria in live cells. Here we demonstrate that IraZolve-Mito can also effectively stain mitochondria in both live and fixed tissue samples. The staining protocol proposed is versatile, providing a universal procedure for cell biologists and pathologists to visualise mitochondria. 2018 Journal Article http://hdl.handle.net/20.500.11937/69167 10.1038/s41598-018-24672-w http://purl.org/au-research/grants/arc/FT130100033 http://creativecommons.org/licenses/by/4.0/ Nature Publishing Group fulltext |
| spellingShingle | Sorvina, A. Bader, C. Darby, J. Lock, M. Soo, J. Johnson, I. Caporale, Chiara Voelcker, N. Stagni, S. Massi, Massimiliano Morrison, J. Plush, S. Brooks, D. Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex |
| title | Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex |
| title_full | Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex |
| title_fullStr | Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex |
| title_full_unstemmed | Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex |
| title_short | Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex |
| title_sort | mitochondrial imaging in live or fixed tissues using a luminescent iridium complex |
| url | http://purl.org/au-research/grants/arc/FT130100033 http://hdl.handle.net/20.500.11937/69167 |