Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition
© 2017 Taylor & Francis. Diet profoundly influences the behavior of animals across many phyla. Despite this, most laboratories using model organisms, such as Drosophila, use multiple, different, commercial or custom-made media for rearing their animals. In addition to measuring growth, fecundi...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/68927 |
| _version_ | 1848761923973152768 |
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| author | Ormerod, K. LePine, O. Abbineni, P. Bridgeman, J. Coorssen, J. Mercier, A. Tattersall, Glenn |
| author_facet | Ormerod, K. LePine, O. Abbineni, P. Bridgeman, J. Coorssen, J. Mercier, A. Tattersall, Glenn |
| author_sort | Ormerod, K. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | © 2017 Taylor & Francis. Diet profoundly influences the behavior of animals across many phyla. Despite this, most laboratories using model organisms, such as Drosophila, use multiple, different, commercial or custom-made media for rearing their animals. In addition to measuring growth, fecundity and longevity, we used several behavioral and physiological assays to determine if and how altering food media influence wild-type (Canton S) Drosophila melanogaster, at larval, pupal, and adult stages. Comparing 2 commonly used commercial food media we observed several key developmental and morphological differences. Third-instar larvae and pupae developmental timing, body weight and size, and even lifespan significantly differed between the 2 diets, and some of these differences persisted into adulthood. Diet was also found to produce significantly different thermal preference, locomotory capacity for geotaxis, feeding rates, and lower muscle response to hormonal stimulation. There were no differences, however, in adult thermal preferences, in the number or viability of eggs laid, or in olfactory learning and memory between the diets. We characterized the composition of the 2 diets and found particularly significant differences in cholesterol and (phospho)lipids between them. Notably, diacylglycerol (DAG) concentrations vary substantially between the 2 diets, and may contribute to key phenotypic differences, including lifespan. Overall, the data confirm that 2 different diets can profoundly influence the behavior, physiology, morphology and development of wild-type Drosophila, with greater behavioral and physiologic differences occurring during the larval stages. |
| first_indexed | 2025-11-14T10:39:23Z |
| format | Journal Article |
| id | curtin-20.500.11937-68927 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:39:23Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-689272018-06-29T12:35:59Z Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition Ormerod, K. LePine, O. Abbineni, P. Bridgeman, J. Coorssen, J. Mercier, A. Tattersall, Glenn © 2017 Taylor & Francis. Diet profoundly influences the behavior of animals across many phyla. Despite this, most laboratories using model organisms, such as Drosophila, use multiple, different, commercial or custom-made media for rearing their animals. In addition to measuring growth, fecundity and longevity, we used several behavioral and physiological assays to determine if and how altering food media influence wild-type (Canton S) Drosophila melanogaster, at larval, pupal, and adult stages. Comparing 2 commonly used commercial food media we observed several key developmental and morphological differences. Third-instar larvae and pupae developmental timing, body weight and size, and even lifespan significantly differed between the 2 diets, and some of these differences persisted into adulthood. Diet was also found to produce significantly different thermal preference, locomotory capacity for geotaxis, feeding rates, and lower muscle response to hormonal stimulation. There were no differences, however, in adult thermal preferences, in the number or viability of eggs laid, or in olfactory learning and memory between the diets. We characterized the composition of the 2 diets and found particularly significant differences in cholesterol and (phospho)lipids between them. Notably, diacylglycerol (DAG) concentrations vary substantially between the 2 diets, and may contribute to key phenotypic differences, including lifespan. Overall, the data confirm that 2 different diets can profoundly influence the behavior, physiology, morphology and development of wild-type Drosophila, with greater behavioral and physiologic differences occurring during the larval stages. 2017 Journal Article http://hdl.handle.net/20.500.11937/68927 10.1080/19336934.2017.1304331 restricted |
| spellingShingle | Ormerod, K. LePine, O. Abbineni, P. Bridgeman, J. Coorssen, J. Mercier, A. Tattersall, Glenn Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition |
| title | Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition |
| title_full | Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition |
| title_fullStr | Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition |
| title_full_unstemmed | Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition |
| title_short | Drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition |
| title_sort | drosophila development, physiology, behavior, and lifespan are influenced by altered dietary composition |
| url | http://hdl.handle.net/20.500.11937/68927 |