Cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by Bifidobacterium species: In-vitro investigations

© 2018 Elsevier Ltd This study aimed to investigate the in-vitro cytotoxicity activity against two cancer cell lines, a-amylase and a-glucosidase inhibitions, ACE-inhibition, antioxidant and proteolytic activities of whole-grain lupin, quinoa and wheat fermented by three species of Bifidobacterium s...

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Main Authors: Ayyash, M., Johnson, Stuart, Liu, S., Al-Mheiri, A., Abushelaibi, A.
Format: Journal Article
Published: Elsevier 2018
Online Access:http://hdl.handle.net/20.500.11937/68846
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author Ayyash, M.
Johnson, Stuart
Liu, S.
Al-Mheiri, A.
Abushelaibi, A.
author_facet Ayyash, M.
Johnson, Stuart
Liu, S.
Al-Mheiri, A.
Abushelaibi, A.
author_sort Ayyash, M.
building Curtin Institutional Repository
collection Online Access
description © 2018 Elsevier Ltd This study aimed to investigate the in-vitro cytotoxicity activity against two cancer cell lines, a-amylase and a-glucosidase inhibitions, ACE-inhibition, antioxidant and proteolytic activities of whole-grain lupin, quinoa and wheat fermented by three species of Bifidobacterium spp. B. animalis, B. breve and B. longum were employed in solid-state fermentation (SSF) 37 °C for 72 h. The ranges of total phenolic contents in fermented lupin (FL), quinoa (FQ) and wheat (FW) were ~17.0 to 44.0, ~11.0 to 36.0, ~5.0–29.0 mg GAE/g, respectively, relative to the unfermented control. a-Glucosidase inhibition increased significantly (p < 0.05) in FL and FQ after 24 h of fermentation. The cereals fermented by B. breve and B. longum had markedly increased ACE-inhibition activities. The magnitude of the cytotoxicity activities in FL was greater (p < 0.05) by approximately 5-fold and 3-fold against colon cancer cell (Caco-2) and breast cancer cell (MCF-7), respectively than in FQ and FW. The findings suggest that health properties of grains could be enhanced by probiotic fermentation and such enhancements depend on grain type and probiotic species. The inclusion of grains fermented by probiotics into foods could improve health benefits of these product.
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institution Curtin University Malaysia
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last_indexed 2025-11-14T10:39:05Z
publishDate 2018
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spelling curtin-20.500.11937-688462018-06-29T12:35:48Z Cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by Bifidobacterium species: In-vitro investigations Ayyash, M. Johnson, Stuart Liu, S. Al-Mheiri, A. Abushelaibi, A. © 2018 Elsevier Ltd This study aimed to investigate the in-vitro cytotoxicity activity against two cancer cell lines, a-amylase and a-glucosidase inhibitions, ACE-inhibition, antioxidant and proteolytic activities of whole-grain lupin, quinoa and wheat fermented by three species of Bifidobacterium spp. B. animalis, B. breve and B. longum were employed in solid-state fermentation (SSF) 37 °C for 72 h. The ranges of total phenolic contents in fermented lupin (FL), quinoa (FQ) and wheat (FW) were ~17.0 to 44.0, ~11.0 to 36.0, ~5.0–29.0 mg GAE/g, respectively, relative to the unfermented control. a-Glucosidase inhibition increased significantly (p < 0.05) in FL and FQ after 24 h of fermentation. The cereals fermented by B. breve and B. longum had markedly increased ACE-inhibition activities. The magnitude of the cytotoxicity activities in FL was greater (p < 0.05) by approximately 5-fold and 3-fold against colon cancer cell (Caco-2) and breast cancer cell (MCF-7), respectively than in FQ and FW. The findings suggest that health properties of grains could be enhanced by probiotic fermentation and such enhancements depend on grain type and probiotic species. The inclusion of grains fermented by probiotics into foods could improve health benefits of these product. 2018 Journal Article http://hdl.handle.net/20.500.11937/68846 10.1016/j.lwt.2018.04.099 Elsevier restricted
spellingShingle Ayyash, M.
Johnson, Stuart
Liu, S.
Al-Mheiri, A.
Abushelaibi, A.
Cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by Bifidobacterium species: In-vitro investigations
title Cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by Bifidobacterium species: In-vitro investigations
title_full Cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by Bifidobacterium species: In-vitro investigations
title_fullStr Cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by Bifidobacterium species: In-vitro investigations
title_full_unstemmed Cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by Bifidobacterium species: In-vitro investigations
title_short Cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by Bifidobacterium species: In-vitro investigations
title_sort cytotoxicity, antihypertensive, antidiabetic and antioxidant activities of solid-state fermented lupin, quinoa and wheat by bifidobacterium species: in-vitro investigations
url http://hdl.handle.net/20.500.11937/68846