Isolation and purification of protein (Conglutin-γ) from lupin seeds
This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with an intermediate pH modulation step enabled the isolation of γ-conglutin with hig...
| Main Author: | |
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| Format: | Thesis |
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Curtin University
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/68387 |
| _version_ | 1848761795598090240 |
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| author | Mane, Sharmilee |
| author_facet | Mane, Sharmilee |
| author_sort | Mane, Sharmilee |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with an intermediate pH modulation
step enabled the isolation of γ-conglutin with highest reported purity in minimum process steps. The effect of process parameters was optimized to obtain the maximum purity. γ-Conglutin purified by the developed process was confirmed for its identity by different analytical techniques. |
| first_indexed | 2025-11-14T10:37:21Z |
| format | Thesis |
| id | curtin-20.500.11937-68387 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:37:21Z |
| publishDate | 2017 |
| publisher | Curtin University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-683872023-06-13T04:12:52Z Isolation and purification of protein (Conglutin-γ) from lupin seeds Mane, Sharmilee This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with an intermediate pH modulation step enabled the isolation of γ-conglutin with highest reported purity in minimum process steps. The effect of process parameters was optimized to obtain the maximum purity. γ-Conglutin purified by the developed process was confirmed for its identity by different analytical techniques. 2017 Thesis http://hdl.handle.net/20.500.11937/68387 Curtin University fulltext |
| spellingShingle | Mane, Sharmilee Isolation and purification of protein (Conglutin-γ) from lupin seeds |
| title | Isolation and purification of protein (Conglutin-γ) from lupin seeds |
| title_full | Isolation and purification of protein (Conglutin-γ) from lupin seeds |
| title_fullStr | Isolation and purification of protein (Conglutin-γ) from lupin seeds |
| title_full_unstemmed | Isolation and purification of protein (Conglutin-γ) from lupin seeds |
| title_short | Isolation and purification of protein (Conglutin-γ) from lupin seeds |
| title_sort | isolation and purification of protein (conglutin-γ) from lupin seeds |
| url | http://hdl.handle.net/20.500.11937/68387 |