Isolation and purification of protein (Conglutin-γ) from lupin seeds
This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with an intermediate pH modulation step enabled the isolation of γ-conglutin with hig...
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| Format: | Thesis |
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Curtin University
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/68387 |
| Summary: | This research focuses on the development of a scalable process for purification of ‘glucose modulating bioactive’, γ-conglutin from lupin seeds. Selective extraction at acidic pH and cation exchange chromatography with an intermediate pH modulation
step enabled the isolation of γ-conglutin with highest reported purity in minimum process steps. The effect of process parameters was optimized to obtain the maximum purity. γ-Conglutin purified by the developed process was confirmed for its identity by different analytical techniques. |
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