Quantification of cell-substratum interactions by atomic force microscopy
Microorganisms adhere to surfaces and, subsequently, form biofilms. This process is of major interest in biotechnology, environmental sciences and medicine. It is crucial to understand the mechanisms of interactions between substratum and cells or biofilms. By combining force mapping-based atomic fo...
| Main Authors: | , , |
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| Format: | Journal Article |
| Published: |
Elsevier BV
2017
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| Online Access: | http://hdl.handle.net/20.500.11937/68139 |
| _version_ | 1848761753387663360 |
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| author | Li, Q. Becker, Thomas Sand, W. |
| author_facet | Li, Q. Becker, Thomas Sand, W. |
| author_sort | Li, Q. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Microorganisms adhere to surfaces and, subsequently, form biofilms. This process is of major interest in biotechnology, environmental sciences and medicine. It is crucial to understand the mechanisms of interactions between substratum and cells or biofilms. By combining force mapping-based atomic force microscopy (AFM) with pyrite-modified cantilevers we quantified the adhesion forces between undenatured planktonic or biofilm cells of Sulfobacillus thermosulfidooxidans and the substratum pyrite with values of 2.6 ± 0.3 nN and 77.3 ± 7.1 pN, respectively. This was achieved under natural conditions without any artefact resulting from the use of denaturing chemicals such as glutaraldehyde. This new technique is unique for quantifying the real interaction forces between cells or biofilms and their substrata. |
| first_indexed | 2025-11-14T10:36:41Z |
| format | Journal Article |
| id | curtin-20.500.11937-68139 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:36:41Z |
| publishDate | 2017 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-681392018-08-14T05:53:58Z Quantification of cell-substratum interactions by atomic force microscopy Li, Q. Becker, Thomas Sand, W. Microorganisms adhere to surfaces and, subsequently, form biofilms. This process is of major interest in biotechnology, environmental sciences and medicine. It is crucial to understand the mechanisms of interactions between substratum and cells or biofilms. By combining force mapping-based atomic force microscopy (AFM) with pyrite-modified cantilevers we quantified the adhesion forces between undenatured planktonic or biofilm cells of Sulfobacillus thermosulfidooxidans and the substratum pyrite with values of 2.6 ± 0.3 nN and 77.3 ± 7.1 pN, respectively. This was achieved under natural conditions without any artefact resulting from the use of denaturing chemicals such as glutaraldehyde. This new technique is unique for quantifying the real interaction forces between cells or biofilms and their substrata. 2017 Journal Article http://hdl.handle.net/20.500.11937/68139 10.1016/j.colsurfb.2017.08.023 Elsevier BV restricted |
| spellingShingle | Li, Q. Becker, Thomas Sand, W. Quantification of cell-substratum interactions by atomic force microscopy |
| title | Quantification of cell-substratum interactions by atomic force microscopy |
| title_full | Quantification of cell-substratum interactions by atomic force microscopy |
| title_fullStr | Quantification of cell-substratum interactions by atomic force microscopy |
| title_full_unstemmed | Quantification of cell-substratum interactions by atomic force microscopy |
| title_short | Quantification of cell-substratum interactions by atomic force microscopy |
| title_sort | quantification of cell-substratum interactions by atomic force microscopy |
| url | http://hdl.handle.net/20.500.11937/68139 |