Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum
Fusarium crown rot (FCR), an important disease of wheat and barley, is mainly caused by Fusarium graminearum, F. culmorum and F. pseudograminearum, which are also responsible for mycotoxin production. This is the first comparative investigation of their colonization on barley plants after stem base...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
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2018
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| Online Access: | http://hdl.handle.net/20.500.11937/68107 |
| _version_ | 1848761744798777344 |
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| author | Pecoraro, F. Giannini, M. Beccari, G. Covarelli, Lorenzo Filippini, G. Pisi, A. Nipoti, P. Prodi, A. |
| author_facet | Pecoraro, F. Giannini, M. Beccari, G. Covarelli, Lorenzo Filippini, G. Pisi, A. Nipoti, P. Prodi, A. |
| author_sort | Pecoraro, F. |
| building | Curtin Institutional Repository |
| collection | Online Access |
| description | Fusarium crown rot (FCR), an important disease of wheat and barley, is mainly caused by Fusarium graminearum, F. culmorum and F. pseudograminearum, which are also responsible for mycotoxin production. This is the first comparative investigation of their colonization on barley plants after stem base inoculation. At plant maturity, FCR symptoms were visually evaluated, fungal biomass was quantified by Real-Time quantitative PCR and deoxynivalenol (DON) was detected by enzyme-linked immunosorbent assay (ELISA). All the inoculated strains caused the typical FCR necrotic symptoms. Real-Time PCR analysis showed that F. graminearum and F. culmorum were present in the head tissues, while F. pseudograminearum colonized only up to the area including the second node of the stem. Conversely, DON was detected up to the head for all the three species. This study shows that, as already demonstrated in previous research for wheat, DON may be detected up to the head as a consequence of stem base infection by the three FCR agents. |
| first_indexed | 2025-11-14T10:36:33Z |
| format | Journal Article |
| id | curtin-20.500.11937-68107 |
| institution | Curtin University Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T10:36:33Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | curtin-20.500.11937-681072018-09-05T00:25:00Z Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum Pecoraro, F. Giannini, M. Beccari, G. Covarelli, Lorenzo Filippini, G. Pisi, A. Nipoti, P. Prodi, A. Fusarium crown rot (FCR), an important disease of wheat and barley, is mainly caused by Fusarium graminearum, F. culmorum and F. pseudograminearum, which are also responsible for mycotoxin production. This is the first comparative investigation of their colonization on barley plants after stem base inoculation. At plant maturity, FCR symptoms were visually evaluated, fungal biomass was quantified by Real-Time quantitative PCR and deoxynivalenol (DON) was detected by enzyme-linked immunosorbent assay (ELISA). All the inoculated strains caused the typical FCR necrotic symptoms. Real-Time PCR analysis showed that F. graminearum and F. culmorum were present in the head tissues, while F. pseudograminearum colonized only up to the area including the second node of the stem. Conversely, DON was detected up to the head for all the three species. This study shows that, as already demonstrated in previous research for wheat, DON may be detected up to the head as a consequence of stem base infection by the three FCR agents. 2018 Journal Article http://hdl.handle.net/20.500.11937/68107 10.23986/afsci.67704 http://creativecommons.org/licenses/by/3.0/ fulltext |
| spellingShingle | Pecoraro, F. Giannini, M. Beccari, G. Covarelli, Lorenzo Filippini, G. Pisi, A. Nipoti, P. Prodi, A. Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum |
| title | Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum |
| title_full | Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum |
| title_fullStr | Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum |
| title_full_unstemmed | Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum |
| title_short | Comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum |
| title_sort | comparative studies about fungal colonization and deoxynivalenol translocation in barley plants inoculated at the base with fusarium graminearum, fusarium culmorum and fusarium pseudograminearum |
| url | http://hdl.handle.net/20.500.11937/68107 |